Q400K2 (MHPD1_PSEPU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-keto-4-pentenoate hydratase 1
EC=4.2.1.80

Alternative name(s):
2-hydroxypentadienoic acid hydratase 1

Gene names

Name:	mhpD1
Synonyms:	orcD

Organism

Pseudomonas putida (Arthrobacter siderocapsulatus)

Taxonomic identifier

303 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas putida group

Protein attributes

Sequence length	265 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of 2-hydroxypentadienoic acid (enolic form of 2-oxopent-4-enoate) to 4-hydroxy-2-ketopentanoic acid By similarity. HAMAP-Rule MF_01655
Catalytic activity	4-hydroxy-2-oxopentanoate = 2-oxopent-4-enoate + H2O. HAMAP-Rule MF_01655
Cofactor	Divalent metal ions By similarity.
Pathway	Aromatic compound metabolism; 3-phenylpropanoate degradation. HAMAP-Rule MF_01655
Sequence similarities	Belongs to the hydratase/decarboxylase family. MhpD subfamily.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Molecular function	Lyase
Gene Ontology (GO)
Biological_process	3-phenylpropionate catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	2-oxopent-4-enoate hydratase activity Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 265	265	2-keto-4-pentenoate hydratase 1 HAMAP-Rule MF_01655		PRO_0000337797

Sequences

	Sequence		Length	Mass (Da)
	Q400K2 [UniParc]. Last modified September 27, 2005. Version 1. Checksum: D27BA5A9A9CA8466	FASTA	265	28,291
	10 20 30 40 50 60 MTISQETLER LAADLRRAEQ QGEAIPPLRD AIGAENGEAA YAIQRLNVAH ALAAGRRLVG 70 80 90 100 110 120 RKVGLTNPKV QAQLGVDQPD FGCLFADMAY GDNETVPFDR VLQPKIEREI ALVLERDLPA 130 140 150 160 170 180 RTPTWPNVAN RHRLGACRPL EIVGTVSPTW NIRFADTVAD NASSGLFVLG GPARRLDGLD 190 200 210 220 230 240 LRGAAMRMTR NDELVSSGSG AECLGHPLNA AVWLARTLAR LGDPLKAGDI VLTGALGPMV 250 260 AVNAGDRFDA EIDGLGRVGV TFSQS

References

[1]

"Cloning of genes involved in meta-cleavage pathways of Pseudomonas putida orc: nucleotide sequences of genes, characterization of two dioxygenases and identification of the orcinol pathway." Straganz G.D., Glieder A., Steiner W. Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ORC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF534914 Genomic DNA. Translation: AAQ10536.1.
3D structure databases
ProteinModelPortal	Q400K2.
SMR	Q400K2. Positions 3-263.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00714.
Family and domain databases
Gene3D	3.90.850.10. 1 hit.
HAMAP	MF_01655. MhpD.
InterPro	IPR002529. Fumarylacetoacetase_C. IPR011234. Fumarylacetoacetase_C-rel. IPR023793. Keto_pentenoate-hydratase. [Graphical view]
Pfam	PF01557. FAA_hydrolase. 1 hit. [Graphical view]
SUPFAM	SSF56529. Fumarylacetoacetase_C-rel. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MHPD1_PSEPU

Accession

Primary (citable) accession number: Q400K2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	September 27, 2005
Last modified:	April 3, 2013
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program