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Protein

Archaemetzincin-1

Gene

AMZ1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Zinc metalloprotease. Exhibits aminopeptidase activity against neurogranin in vitro. Does not hydrolyze angiotensin-2.1 Publication

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit. One is catalytic, whereas the other seems to have a structural role.By similarity

Enzyme regulationi

Inhibited by the general metalloprotease inhibitors o-phenanthroline and batimastat. Also significantly inhibited by amastatin, which is an inhibitor of aminopeptidases. Not inhibited by 4-(2-aminoethyl)-benzenesulfonyl fluoride, E-64, and TIMPS (tissue inhibitors of metalloproteinases), which are inhibitors of serine, cysteine, and matrix metalloproteases, respectively.1 Publication

pH dependencei

Optimum pH is 8.0.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi261 – 2611Zinc 1; via pros nitrogen; catalyticBy similarity
Active sitei262 – 2621Proton acceptorPROSITE-ProRule annotation
Metal bindingi265 – 2651Zinc 1; via pros nitrogen; catalyticBy similarity
Metal bindingi272 – 2721Zinc 2By similarity
Metal bindingi277 – 2771Zinc 2By similarity
Metal bindingi296 – 2961Zinc 2By similarity
Metal bindingi299 – 2991Zinc 2By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM54.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Archaemetzincin-1 (EC:3.4.-.-)
Alternative name(s):
Archeobacterial metalloproteinase-like protein 1
Gene namesi
Name:AMZ1
Synonyms:KIAA1950
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:22231. AMZ1.

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162376413.

Polymorphism and mutation databases

BioMutaiAMZ1.
DMDMi85540611.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 498498Archaemetzincin-1PRO_0000159614Add
BLAST

Proteomic databases

PaxDbiQ400G9.
PRIDEiQ400G9.

PTM databases

iPTMnetiQ400G9.
PhosphoSiteiQ400G9.

Expressioni

Tissue specificityi

Predominantly expressed in heart and liver. Also expressed at lower level in kidney, pancreas and testis. Expressed in fetal tissues such as kidney, liver, lung and brain.1 Publication

Gene expression databases

BgeeiQ400G9.
CleanExiHS_AMZ1.
ExpressionAtlasiQ400G9. baseline and differential.
GenevisibleiQ400G9. HS.

Organism-specific databases

HPAiHPA020233.

Interactioni

Protein-protein interaction databases

BioGridi127579. 12 interactions.
STRINGi9606.ENSP00000308149.

Structurei

3D structure databases

ProteinModelPortaliQ400G9.
SMRiQ400G9. Positions 256-309.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M54 family.Curated

Phylogenomic databases

eggNOGiENOG410IVMN. Eukaryota.
COG1913. LUCA.
GeneTreeiENSGT00530000063996.
HOGENOMiHOG000220860.
HOVERGENiHBG079854.
InParanoidiQ400G9.
KOiK06974.
OMAiARKHIYL.
OrthoDBiEOG7WX08V.
PhylomeDBiQ400G9.
TreeFamiTF328603.

Family and domain databases

PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q400G9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLQCRPAQEF SFGPRALKDA LVSTDAALQQ LYVSAFSPAE RLFLAEAYNP
60 70 80 90 100
QRTLFCTLLI RTGFDWLLSR PEAPEDFQTF HASLQHRKPR LARKHIYLQP
110 120 130 140 150
IDLSEEPVGS SLLHQLCSCT EAFFLGLRVK CLPSVAAASI RCSSRPSRDS
160 170 180 190 200
DRLQLHTDGI LSFLKNNKPG DALCVLGLTL SDLYPHEAWS FTFSKFLPGH
210 220 230 240 250
EVGVCSFARF SGEFPKSGPS APDLALVEAA ADGPEAPLQD RGWALCFSAL
260 270 280 290 300
GMVQCCKVTC HELCHLLGLG NCRWLRCLMQ GALSLDEALR RPLDLCPICL
310 320 330 340 350
RKLQHVLGFR LIERYQRLYT WTQAVVGTWP SQEAGEPSVW EDTPPASADS
360 370 380 390 400
GMCCESDSEP GTSVSEPLTP DAGSHTFASG PEEGLSYLAA SEAPLPPGGP
410 420 430 440 450
AEAIKEHERW LAMCIQALQR EVAEEDLVQV DRAVDALDRW EMFTGQLPAT
460 470 480 490
RQDPPSSRDS VGLRKVLGDK FSSLRRKLSA RKLARAESAP RPWDGEES
Length:498
Mass (Da):54,924
Last modified:September 27, 2005 - v1
Checksum:i64FB132F82CB0E0C
GO
Isoform 2 (identifier: Q400G9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     201-297: EVGVCSFARF...ALRRPLDLCP → GHVPRALPPS...ACQRRLGHVL
     298-498: Missing.

Note: No experimental confirmation available.
Show »
Length:297
Mass (Da):31,860
Checksum:iADA2FF44EF027AEF
GO

Sequence cautioni

The sequence BAB85536.1 differs from that shown. Reason: Erroneous initiation. Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti491 – 4911R → H.
Corresponds to variant rs7776970 [ dbSNP | Ensembl ].
VAR_024849

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei201 – 29797EVGVC…LDLCP → GHVPRALPPSGPGELPLAPL PHAGCAQPGRGPAAAPGPLS HLPEEAAACPGFQAHREVPE TLHLDSGGGGDVAQPGGGGA VSVGGHPACQRRLGHVL in isoform 2. 1 PublicationVSP_055187Add
BLAST
Alternative sequencei298 – 498201Missing in isoform 2. 1 PublicationVSP_055188Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ635357 mRNA. Translation: CAG25749.1.
AB075830 mRNA. Translation: BAB85536.1. Different initiation.
AK092107 mRNA. Translation: BAG52482.1.
AC006028 Genomic DNA. No translation available.
CCDSiCCDS34589.1. [Q400G9-1]
CCDS64582.1. [Q400G9-2]
RefSeqiNP_001271284.1. NM_001284355.2. [Q400G9-2]
NP_597720.1. NM_133463.3. [Q400G9-1]
XP_011513452.1. XM_011515150.1. [Q400G9-1]
UniGeneiHs.42221.

Genome annotation databases

EnsembliENST00000312371; ENSP00000308149; ENSG00000174945. [Q400G9-1]
ENST00000407112; ENSP00000386020; ENSG00000174945. [Q400G9-2]
GeneIDi155185.
KEGGihsa:155185.
UCSCiuc003smr.3. human. [Q400G9-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ635357 mRNA. Translation: CAG25749.1.
AB075830 mRNA. Translation: BAB85536.1. Different initiation.
AK092107 mRNA. Translation: BAG52482.1.
AC006028 Genomic DNA. No translation available.
CCDSiCCDS34589.1. [Q400G9-1]
CCDS64582.1. [Q400G9-2]
RefSeqiNP_001271284.1. NM_001284355.2. [Q400G9-2]
NP_597720.1. NM_133463.3. [Q400G9-1]
XP_011513452.1. XM_011515150.1. [Q400G9-1]
UniGeneiHs.42221.

3D structure databases

ProteinModelPortaliQ400G9.
SMRiQ400G9. Positions 256-309.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi127579. 12 interactions.
STRINGi9606.ENSP00000308149.

Protein family/group databases

MEROPSiM54.003.

PTM databases

iPTMnetiQ400G9.
PhosphoSiteiQ400G9.

Polymorphism and mutation databases

BioMutaiAMZ1.
DMDMi85540611.

Proteomic databases

PaxDbiQ400G9.
PRIDEiQ400G9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000312371; ENSP00000308149; ENSG00000174945. [Q400G9-1]
ENST00000407112; ENSP00000386020; ENSG00000174945. [Q400G9-2]
GeneIDi155185.
KEGGihsa:155185.
UCSCiuc003smr.3. human. [Q400G9-1]

Organism-specific databases

CTDi155185.
GeneCardsiAMZ1.
HGNCiHGNC:22231. AMZ1.
HPAiHPA020233.
MIMi615168. gene.
neXtProtiNX_Q400G9.
PharmGKBiPA162376413.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IVMN. Eukaryota.
COG1913. LUCA.
GeneTreeiENSGT00530000063996.
HOGENOMiHOG000220860.
HOVERGENiHBG079854.
InParanoidiQ400G9.
KOiK06974.
OMAiARKHIYL.
OrthoDBiEOG7WX08V.
PhylomeDBiQ400G9.
TreeFamiTF328603.

Miscellaneous databases

GenomeRNAii155185.
PROiQ400G9.
SOURCEiSearch...

Gene expression databases

BgeeiQ400G9.
CleanExiHS_AMZ1.
ExpressionAtlasiQ400G9. baseline and differential.
GenevisibleiQ400G9. HS.

Family and domain databases

PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of human archaemetzincin-1 and - 2, two novel members of a family of metalloproteases widely distributed in Archaea."
    Diaz-Perales A., Quesada V., Peinado J.R., Ugalde A.P., Alvarez J., Suarez M.F., Gomis-Rueth X., Lopez-Otin C.
    J. Biol. Chem. 280:30367-30375(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, TISSUE SPECIFICITY.
  2. "Prediction of the coding sequences of unidentified human genes. XXII. The complete sequences of 50 new cDNA clones which code for large proteins."
    Nagase T., Kikuno R., Ohara O.
    DNA Res. 8:319-327(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  4. "The DNA sequence of human chromosome 7."
    Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L.
    , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
    Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiAMZ1_HUMAN
AccessioniPrimary (citable) accession number: Q400G9
Secondary accession number(s): B3KRS0, Q8TF51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: September 27, 2005
Last modified: June 8, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Peptidase families
    Classification of peptidase families and list of entries
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.