Reviewed, UniProtKB/Swiss-Prot Q400G9 (AMZ1_HUMAN)

Names and origin

Protein names

Recommended name:
    Archaemetzincin-1
    EC=3.-.-.-
Alternative name(s):
    Archeobacterial metalloproteinase-like protein 1

Gene names

Name:	AMZ1
Synonyms:	KIAA1950

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	498 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Zinc metalloprotease. Exhibits aminopeptidase activity against neurogranin in vitro. Does not hydrolyze angiotensin-2. Ref.1
Enzyme regulation	Inhibited by the general metalloprotease inhibitors o-phenanthroline and batimastat. Also significantly inhibited by amastatin, which is an inhibitor of aminopeptidases. Not inhibited by 4-(2-aminoethyl)-benzenesulfonyl fluoride, E-64, and TIMPS (tissue inhibitors of metalloproteinases), which are inhibitors of serine, cysteine, and matrix metalloproteases, respectively. Ref.1
Tissue specificity	Predominantly expressed in heart and liver. Also expressed at lower level in kidney, pancreas and testis. Expressed in fetal tissues such as kidney, liver, lung and brain. Ref.1
Sequence similarities	Belongs to the peptidase M54 family.
Biophysicochemical properties	pH dependence: Optimum pH is 8.0.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 498	498	Archaemetzincin-1		PRO_0000159614
Sites
	Active site	262	1	By similarity
	Metal binding	261	1	Zinc; catalytic By similarity
	Metal binding	265	1	Zinc; catalytic By similarity
	Metal binding	272	1	Zinc; catalytic Potential
Natural variations
	Natural variant	491	1	R → H: dbSNP rs7776970.		VAR_024849

Sequences

	Sequence		Length	Mass (Da)
	Q400G9-1 [UniParc]. Last modified September 27, 2005. Version 1. Checksum: 64FB132F82CB0E0C	FASTA	498	54,924
	10 20 30 40 50 60 MLQCRPAQEF SFGPRALKDA LVSTDAALQQ LYVSAFSPAE RLFLAEAYNP QRTLFCTLLI 70 80 90 100 110 120 RTGFDWLLSR PEAPEDFQTF HASLQHRKPR LARKHIYLQP IDLSEEPVGS SLLHQLCSCT 130 140 150 160 170 180 EAFFLGLRVK CLPSVAAASI RCSSRPSRDS DRLQLHTDGI LSFLKNNKPG DALCVLGLTL 190 200 210 220 230 240 SDLYPHEAWS FTFSKFLPGH EVGVCSFARF SGEFPKSGPS APDLALVEAA ADGPEAPLQD 250 260 270 280 290 300 RGWALCFSAL GMVQCCKVTC HELCHLLGLG NCRWLRCLMQ GALSLDEALR RPLDLCPICL 310 320 330 340 350 360 RKLQHVLGFR LIERYQRLYT WTQAVVGTWP SQEAGEPSVW EDTPPASADS GMCCESDSEP 370 380 390 400 410 420 GTSVSEPLTP DAGSHTFASG PEEGLSYLAA SEAPLPPGGP AEAIKEHERW LAMCIQALQR 430 440 450 460 470 480 EVAEEDLVQV DRAVDALDRW EMFTGQLPAT RQDPPSSRDS VGLRKVLGDK FSSLRRKLSA 490 RKLARAESAP RPWDGEES

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of human archaemetzincin-1 and - 2, two novel members of a family of metalloproteases widely distributed in Archaea." Diaz-Perales A., Quesada V., Peinado J.R., Ugalde A.P., Alvarez J., Suarez M.F., Gomis-Rueth X., Lopez-Otin C. J. Biol. Chem. 280:30367-30375(2005) [PubMed: 15972818] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, TISSUE SPECIFICITY.
[2]	"Prediction of the coding sequences of unidentified human genes. XXII. The complete sequences of 50 new cDNA clones which code for large proteins." Nagase T., Kikuno R., Ohara O. DNA Res. 8:319-327(2001) [PubMed: 11853319] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ635357 mRNA. Translation: CAG25749.1. AB075830 mRNA. Translation: BAB85536.1. Different initiation.
IPI	IPI00291851.
RefSeq	NP_597720.1.
UniGene	Hs.42221
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	M54.950.
Genome annotation databases
Ensembl	ENST00000312371; ENSP00000308149; ENSG00000174945; Homo sapiens. [Genome view]
GeneID	155185.
KEGG	hsa:155185.
UCSC	uc003smr.1. human.
Organism-specific databases
CTD	155185.
GeneCards	GC07P002685.
H-InvDB	HIX0006437.
HGNC	HGNC:22231. AMZ1.
HPA	HPA020233.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q400G9.
HOVERGEN	Q400G9.
OMA	LQPIDLS
OrthoDB	EOG91VNNF
Enzyme and pathway databases
BioCyc	CATTLE:ENSBTAG00000026194-MON.
Gene expression databases
ArrayExpress	Q400G9.
Bgee	Q400G9.
CleanEx	HS_AMZ1.
Genevestigator	Q400G9.
GermOnline	ENSG00000174945. Homo sapiens.
Family and domain databases
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	87389.

Entry information

Entry name

AMZ1_HUMAN

Accession

Primary (citable) accession number: Q400G9
Secondary accession number(s): Q8TF51

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	September 27, 2005
Last modified:	November 24, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.