Reviewed, UniProtKB/Swiss-Prot Q400C8 (AMZ2_MOUSE)

Names and origin

Protein names

Recommended name:
    Archaemetzincin-2
    EC=3.-.-.-
Alternative name(s):
    Archeobacterial metalloproteinase-like protein 2

Gene names

Name:

Amz2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Zinc metalloprotease. Exhibits activity against angiotensin-3 in vitro. Does not hydrolyze neurogranin nor angiotensin-2 By similarity.
Enzyme regulation	Inhibited by the general metalloprotease inhibitors o-phenanthroline and batimastat. Also significantly inhibited by amastatin, which is an inhibitor of aminopeptidases. Not inhibited by 4-(2-aminoethyl)-benzenesulfonyl fluoride, E-64, and TIMPS (tissue inhibitors of metalloproteinases), which are inhibitors of serine, cysteine, and matrix metalloproteases, respectively By similarity.
Sequence similarities	Belongs to the peptidase archaemetzincin family.
Sequence caution	The sequence BAB23148.1 differs from that shown. Reason: Frameshift at positions 47 and 147.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q400C8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q400C8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     310-321: ALVKWIDDESCN → MNEERLQQQLQI
     322-359: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 359	359	Archaemetzincin-2		PRO_0000159619
Sites
	Active site	255	1	By similarity
	Metal binding	254	1	Zinc; catalytic By similarity
	Metal binding	258	1	Zinc; catalytic By similarity
	Metal binding	265	1	Zinc; catalytic Potential
Natural variations
	Alternative sequence	310 – 321	12	ALVKW…DESCN → MNEERLQQQLQI in isoform 2.		VSP_016984
	Alternative sequence	322 – 359	38	Missing in isoform 2.		VSP_016985

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified September 27, 2005. Version 1. Checksum: 909A226405DBDF4B	FASTA	359	41,341
	10 20 30 40 50 60 MQVLRHSEHT LKTALLSKNP VLVSQYEKLD AGEQRLMNEA FQPRSNLFEP ITVHSQSDWI 70 80 90 100 110 120 SSHPEAPQDF EQFFSDRYRK APCPKKHIIY IQSIGSLGNT RVISEEYIKW LKGYCEAFFY 130 140 150 160 170 180 GLKVKFLEPV SVSETKCSFR VNEHTQNLQI HTGHILAFLK KNKPEDAFCI VGITMIDLYP 190 200 210 220 230 240 RDSWNFVFGQ ASLSSGVGIF SFARYGKDFY TSKYEGNVTS LQLTSPTDYS IFDNYYIPEI 250 260 270 280 290 300 TSVLLLRSCK TLTHEIGHIL GLRHCQWLAC LMQGSNHLEE SDRRPLNVCP ICLRKLQSAI 310 320 330 340 350 GFNIVERYRA LVKWIDDESC NESGATPKSS SEHAHLPKPV EAFKDWREWL MRCIALLEK
	Isoform 2. Checksum: 4AF1AF1B9B24AE7B	FASTA	321	37,117
	10 20 30 40 50 60 MQVLRHSEHT LKTALLSKNP VLVSQYEKLD AGEQRLMNEA FQPRSNLFEP ITVHSQSDWI 70 80 90 100 110 120 SSHPEAPQDF EQFFSDRYRK APCPKKHIIY IQSIGSLGNT RVISEEYIKW LKGYCEAFFY 130 140 150 160 170 180 GLKVKFLEPV SVSETKCSFR VNEHTQNLQI HTGHILAFLK KNKPEDAFCI VGITMIDLYP 190 200 210 220 230 240 RDSWNFVFGQ ASLSSGVGIF SFARYGKDFY TSKYEGNVTS LQLTSPTDYS IFDNYYIPEI 250 260 270 280 290 300 TSVLLLRSCK TLTHEIGHIL GLRHCQWLAC LMQGSNHLEE SDRRPLNVCP ICLRKLQSAI 310 320 GFNIVERYRM NEERLQQQLQ I

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of human archaemetzincin-1 and - 2, two novel members of a family of metalloproteases widely distributed in Archaea." Diaz-Perales A., Quesada V., Peinado J.R., Ugalde A.P., Alvarez J., Suarez M.F., Gomis-Rueth X., Lopez-Otin C. J. Biol. Chem. 280:30367-30375(2005) [PubMed: 15972818] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: C57BL/6.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6J. Tissue: Testis.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: 129. Tissue: Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ879914 mRNA. Translation: CAI53759.1. AK004062 mRNA. Translation: BAB23148.1. Frameshift. AK075748 mRNA. Translation: BAC35928.1. Different initiation. AK077195 mRNA. Translation: BAC36674.1. Different initiation. BC025087 mRNA. Translation: AAH25087.1. Different initiation.
IPI	IPI00336976. IPI00625349.
UniGene	Mm.265874 Mm.291901
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q400C8.
Genome annotation databases
Ensembl	ENSMUSG00000020610. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:104837. Amz2.
Phylogenomic databases
HOGENOM	Q400C8.
HOVERGEN	Q400C8.
Gene expression databases
ArrayExpress	Q400C8.
Bgee	Q400C8.
CleanEx	MM_AMZ2.
GermOnline	ENSMUSG00000020610. Mus musculus.
Family and domain databases
InterPro	IPR012962. Pept_M54_archaemetzncn. IPR006025. Pept_M_Zn_BS. [Graphical view]
Pfam	PF07998. Peptidase_M54. 1 hit. [Graphical view]
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

AMZ2_MOUSE

Accession

Primary (citable) accession number: Q400C8
Secondary accession number(s): Q8BVM5, Q8K3B9, Q9D121

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	September 27, 2005
Last modified:	June 16, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)