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Protein

Archaemetzincin-2

Gene

Amz2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Zinc metalloprotease. Exhibits activity against angiotensin-3 in vitro. Does not hydrolyze neurogranin nor angiotensin-2 (By similarity).By similarity

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit. One is catalytic, whereas the other seems to have a structural role.By similarity

Enzyme regulationi

Inhibited by the general metalloprotease inhibitors o-phenanthroline and batimastat. Also significantly inhibited by amastatin, which is an inhibitor of aminopeptidases. Not inhibited by 4-(2-aminoethyl)-benzenesulfonyl fluoride, E-64, and TIMPS (tissue inhibitors of metalloproteinases), which are inhibitors of serine, cysteine, and matrix metalloproteases, respectively (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi254 – 2541Zinc 1; via pros nitrogen; catalyticBy similarity
Active sitei255 – 2551Proton acceptorPROSITE-ProRule annotation
Metal bindingi258 – 2581Zinc 1; via pros nitrogen; catalyticBy similarity
Metal bindingi264 – 2641Zinc 1; via pros nitrogen; catalyticBy similarity
Metal bindingi265 – 2651Zinc 2By similarity
Metal bindingi270 – 2701Zinc 2By similarity
Metal bindingi289 – 2891Zinc 2By similarity
Metal bindingi292 – 2921Zinc 2By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM54.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Archaemetzincin-2 (EC:3.4.-.-)
Alternative name(s):
Archeobacterial metalloproteinase-like protein 2
Gene namesi
Name:Amz2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:104837. Amz2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 359359Archaemetzincin-2PRO_0000159619Add
BLAST

Proteomic databases

EPDiQ400C8.
MaxQBiQ400C8.
PaxDbiQ400C8.
PRIDEiQ400C8.

PTM databases

iPTMnetiQ400C8.
PhosphoSiteiQ400C8.

Expressioni

Tissue specificityi

Predominantly expressed in testis.1 Publication

Developmental stagei

Expression in testis begins at about postnatal day 10 (P10), with adult level of expression reached at P20.1 Publication

Gene expression databases

BgeeiENSMUSG00000020610.
CleanExiMM_AMZ2.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000090157.

Structurei

3D structure databases

ProteinModelPortaliQ400C8.
SMRiQ400C8. Positions 170-297.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M54 family.Curated

Phylogenomic databases

eggNOGiENOG410IVMN. Eukaryota.
COG1913. LUCA.
HOGENOMiHOG000293350.
HOVERGENiHBG080165.
InParanoidiQ400C8.
KOiK06974.

Family and domain databases

CDDicd11375. Peptidase_M54. 1 hit.
Gene3Di3.40.390.10. 1 hit.
InterProiIPR024079. MetalloPept_cat_dom.
IPR012962. Pept_M54_archaemetzincn.
[Graphical view]
PfamiPF07998. Peptidase_M54. 1 hit.
[Graphical view]
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q400C8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQVLRHSEHT LKTALLSKNP VLVSQYEKLD AGEQRLMNEA FQPRSNLFEP
60 70 80 90 100
ITVHSQSDWI SSHPEAPQDF EQFFSDRYRK APCPKKHIIY IQSIGSLGNT
110 120 130 140 150
RVISEEYIKW LKGYCEAFFY GLKVKFLEPV SVSETKCSFR VNEHTQNLQI
160 170 180 190 200
HTGHILAFLK KNKPEDAFCI VGITMIDLYP RDSWNFVFGQ ASLSSGVGIF
210 220 230 240 250
SFARYGKDFY TSKYEGNVTS LQLTSPTDYS IFDNYYIPEI TSVLLLRSCK
260 270 280 290 300
TLTHEIGHIL GLRHCQWLAC LMQGSNHLEE SDRRPLNVCP ICLRKLQSAI
310 320 330 340 350
GFNIVERYRA LVKWIDDESC NESGATPKSS SEHAHLPKPV EAFKDWREWL

MRCIALLEK
Length:359
Mass (Da):41,341
Last modified:September 27, 2005 - v1
Checksum:i909A226405DBDF4B
GO
Isoform 2 (identifier: Q400C8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     310-321: ALVKWIDDESCN → MNEERLQQQLQI
     322-359: Missing.

Note: No experimental confirmation available.
Show »
Length:321
Mass (Da):37,117
Checksum:i4AF1AF1B9B24AE7B
GO

Sequence cautioni

The sequence AAH25087 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAB23148 differs from that shown. Reason: Frameshift at positions 47 and 147. Curated
The sequence BAC35928 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAC36674 differs from that shown. Reason: Erroneous initiation. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei310 – 32112ALVKW…DESCN → MNEERLQQQLQI in isoform 2. 1 PublicationVSP_016984Add
BLAST
Alternative sequencei322 – 35938Missing in isoform 2. 1 PublicationVSP_016985Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ879914 mRNA. Translation: CAI53759.1.
AK004062 mRNA. Translation: BAB23148.1. Frameshift.
AK075748 mRNA. Translation: BAC35928.1. Different initiation.
AK077195 mRNA. Translation: BAC36674.1. Different initiation.
BC025087 mRNA. Translation: AAH25087.1. Different initiation.
RefSeqiNP_001239122.1. NM_001252193.1.
NP_079551.3. NM_025275.4.
UniGeneiMm.211850.

Genome annotation databases

GeneIDi13929.
KEGGimmu:13929.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ879914 mRNA. Translation: CAI53759.1.
AK004062 mRNA. Translation: BAB23148.1. Frameshift.
AK075748 mRNA. Translation: BAC35928.1. Different initiation.
AK077195 mRNA. Translation: BAC36674.1. Different initiation.
BC025087 mRNA. Translation: AAH25087.1. Different initiation.
RefSeqiNP_001239122.1. NM_001252193.1.
NP_079551.3. NM_025275.4.
UniGeneiMm.211850.

3D structure databases

ProteinModelPortaliQ400C8.
SMRiQ400C8. Positions 170-297.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000090157.

Protein family/group databases

MEROPSiM54.002.

PTM databases

iPTMnetiQ400C8.
PhosphoSiteiQ400C8.

Proteomic databases

EPDiQ400C8.
MaxQBiQ400C8.
PaxDbiQ400C8.
PRIDEiQ400C8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi13929.
KEGGimmu:13929.

Organism-specific databases

CTDi51321.
MGIiMGI:104837. Amz2.

Phylogenomic databases

eggNOGiENOG410IVMN. Eukaryota.
COG1913. LUCA.
HOGENOMiHOG000293350.
HOVERGENiHBG080165.
InParanoidiQ400C8.
KOiK06974.

Miscellaneous databases

PROiQ400C8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020610.
CleanExiMM_AMZ2.

Family and domain databases

CDDicd11375. Peptidase_M54. 1 hit.
Gene3Di3.40.390.10. 1 hit.
InterProiIPR024079. MetalloPept_cat_dom.
IPR012962. Pept_M54_archaemetzincn.
[Graphical view]
PfamiPF07998. Peptidase_M54. 1 hit.
[Graphical view]
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAMZ2_MOUSE
AccessioniPrimary (citable) accession number: Q400C8
Secondary accession number(s): Q8BVM5, Q8K3B9, Q9D121
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: September 27, 2005
Last modified: September 7, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.