Xylose isomerase - Hordeum vulgare (Barley)

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Reviewed, UniProtKB/Swiss-Prot Q40082 (XYLA_HORVU)

Last modified June 16, 2009. Version 54. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names

Recommended name:
Xylose isomerase
EC=5.3.1.5

Gene names

Name:

XYLA

Organism

Hordeum vulgare (Barley)

Taxonomic identifier

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length	479 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	D-xylose = D-xylulose.
Cofactor	Binds 2 manganese ions per subunit.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the xylose isomerase family.
biophysicochemical properties	pH dependence: Optimum pH is 7.0-9.0. Temperature dependence: Optimum temperature is 60 degrees Celsius.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Pentose shunt Xylose metabolism
Ligand	Magnesium Manganese Metal-binding
Molecular function	Isomerase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	D-xylose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pentose-phosphate shunt Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW xylose isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

479

Xylose isomerase

PRO_0000195825

Sites

Active site

1

By similarity

Metal binding

1

Manganese 1 By similarity

Metal binding

1

Manganese 1 By similarity

Metal binding

1

Manganese 2 By similarity

Metal binding

1

Manganese 2 By similarity

Metal binding

1

Manganese 1 By similarity

Metal binding

1

Manganese 2 By similarity

Metal binding

1

Manganese 2 By similarity

Metal binding

1

Manganese 1 By similarity

Experimental info

Sequence conflict

26

AGKGD…PSGKQ → GSSSFTAQCKYISAPMIVPY DLLLPA in CAA64544. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q40082-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 57E6033AFD77A33F
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479

53,614

        10         20         30         40         50         60 
MKGGELLVLL LASSLCLSAA VAAQETCPAD IGAKCTDAAS DDWEGEFFPG IDKINYEGPT 

        70         80         90        100        110        120 
SKKPLSYKWY NAEEVILGKK MKDWFRFSVA FWHTFRGTGG DPFGAPTKNW PWEDGTNSLA 

       130        140        150        160        170        180 
MAKRRMKAHF EFMEKLGVER WCFHDRDIAP DGKTLAETNA NLDEIVELAK QLQSETNIKP 

       190        200        210        220        230        240 
LWGTAQLFMH PRYMHGAATS PEVKVYAYAA AQVKKALEVT HYLGGENYVF WGGREGYQTL 

       250        260        270        280        290        300 
LNTDMKRELE HLANFLQAAV NHKKKIGFNG TLLIEPKPQE PTKHQYDWDV ATTFSFLQKF 

       310        320        330        340        350        360 
GLTGEFKINV ECNHATLSGH SCHHELETAR INDILGNIDA NTGDPQVGWD TDEFLTDISE 

       370        380        390        400        410        420 
ATLIMSSVVK NDGLAPGGFN FYAKLRREST DVEDLFIAHI SGMDTMARGR RNVVKLIEDG 

       430        440        450        460        470 
SLDELVRKRY QSFDTEIGAM IEAGKGDFET LEKKALEWGE PTVPSGKQEL AEMLFQSAL

References

[1]

"Protein purification, and cloning and characterization of the cDNA and gene for xylose isomerase of barley."
Kristo P.A., Saarelainen R., Fagerstroem R., Aho S., Korhola M.
Eur. J. Biochem. 237:240-246(1996) [PubMed: 8620879] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION.
Strain: cv. Himalaya.

Cross-references

Sequence databases
	X95257 mRNA. Translation: CAA64545.1. X95256 Genomic DNA. Translation: CAA64544.1.
PIR	S65466.
3D structure databases
HSSP	HSSP built from PDB template 1A0E based on UniProtKB P45687.
ModBase	Search...
Organism-specific databases
Gramene	Q40082.
Enzyme and pathway databases
BRENDA	5.3.1.5. 283.
Family and domain databases
InterPro	IPR013022. Xyl_isomerase-like_TIM-brl. IPR012307. Xyl_isomerase-typ_TIM-brl. IPR013452. Xylose_isom_bac. IPR001998. Xylose_isomerase. IPR018115. Xylose_isomerase_AS. [Graphical view]
Gene3D	G3DSA:3.20.20.150. Xyl_isomerase-like_TIM-brl. 1 hit.
Pfam	PF01261. AP_endonuc_2. 1 hit. [Graphical view]
PRINTS	PR00688. XYLOSISMRASE.
TIGRFAMs	TIGR02630. xylose_isom_A. 1 hit.
PROSITE	PS51415. XYLOSE_ISOMERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XYLA_HORVU

Accession

Primary (citable) accession number: Q40082
Secondary accession number(s): Q40081

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents