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Protein

Alpha-amylase

Gene
N/A
Organism
Hordeum vulgare (Barley)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.UniRule annotation

Cofactori

Ca2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei203NucleophileUniRule annotation1
Active sitei228Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylaseUniRule annotation (EC:3.2.1.1UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolaseUniRule annotation
OrganismiHordeum vulgare (Barley)Imported
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladePooideaeTriticodaeTriticeaeHordeinaeHordeum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23UniRule annotationAdd BLAST23
ChainiPRO_501060538224 – 437Alpha-amylaseUniRule annotationAdd BLAST414

Structurei

3D structure databases

ProteinModelPortaliQ40018.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 366AamyInterPro annotationAdd BLAST342
Domaini367 – 427Alpha-amyl_C2InterPro annotationAdd BLAST61

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.UniRule annotation

Keywords - Domaini

SignalUniRule annotation

Phylogenomic databases

eggNOGiKOG0471. Eukaryota.
COG0366. LUCA.

Family and domain databases

InterProiView protein in InterPro
IPR012850. A-amylase_bs_C.
IPR013775. A-amylase_pln.
IPR006047. Glyco_hydro_13_cat_dom.
IPR017853. Glycoside_hydrolase_SF.
PfamiView protein in Pfam
PF07821. Alpha-amyl_C2. 1 hit.
PF00128. Alpha-amylase. 1 hit.
PIRSFiPIRSF001028. Alph-amls_plant. 1 hit.
SMARTiView protein in SMART
SM00642. Aamy. 1 hit.
SM00810. Alpha-amyl_C2. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Q40018-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKKSHICCF SLLLLLFAGL ASGHQVLFQG FNWESWKQSG GWYNMMLGKV
60 70 80 90 100
DDIAAAGVTH VWLPPPSYSV AGEGYMPGRL YDIDASKYGN AAELKSLIGA
110 120 130 140 150
LHGNGVQAIA DIVINHLCAD YKDSRGIYCI YEGGTSDSRL DWGPHMICRD
160 170 180 190 200
DTKYSDGTAN LDTRADYADA PDIDHLNHRV QSELKEWLLW LKSDLGFDAW
210 220 230 240 250
PLDFARGYSA EMAKVYIDGT SPSLAVAEIW DGMVPGGDGK PNYDQDAHRQ
260 270 280 290 300
NLVNWVDKVG GRASAGMVFD FTTKGILNVA VEGELWRLIG PASKAPGVMG
310 320 330 340 350
WWPAKAVTFI DNHDTGSNGS KWPVPSDKIM QGYAYILTHS GIPCIFYDHF
360 370 380 390 400
FNLGFKDEIA ALVAIRKRNG ITATSALKIL MHDGDAYVAE IDSKVVVKIG
410 420 430
SRYELGAVIP ARFVTSANGK DYAVWEKIAA GPTLQRS
Length:437
Mass (Da):47,811
Last modified:November 1, 1996 - v1
Checksum:i68866B0A1A650656
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17127 Genomic DNA. Translation: AAA32928.1.
PIRiS07040.

Similar proteinsi

Entry informationi

Entry nameiQ40018_HORVU
AccessioniPrimary (citable) accession number: Q40018
Entry historyiIntegrated into UniProtKB/TrEMBL: November 1, 1996
Last sequence update: November 1, 1996
Last modified: September 27, 2017
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)