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Protein

Alpha-amylase

Gene
N/A
Organism
Hordeum vulgare (Barley)
Status
Unreviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.UniRule annotation

Cofactori

Ca2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei203NucleophileUniRule annotation1
Active sitei228Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidaseUniRule annotation, Hydrolase
Biological processCarbohydrate metabolismUniRule annotation

Protein family/group databases

CAZyiGH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylaseUniRule annotation (EC:3.2.1.1UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolaseUniRule annotation
OrganismiHordeum vulgare (Barley)Imported
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladePooideaeTriticodaeTriticeaeHordeinaeHordeum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24UniRule annotationAdd BLAST24
ChainiPRO_501060528325 – 427Alpha-amylaseUniRule annotationAdd BLAST403

Expressioni

Gene expression databases

ExpressionAtlasiQ40015 baseline

Structurei

3D structure databases

ProteinModelPortaliQ40015
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 365AamyInterPro annotationAdd BLAST341
Domaini366 – 426Alpha-amyl_C2InterPro annotationAdd BLAST61

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.UniRule annotation

Keywords - Domaini

SignalUniRule annotation

Family and domain databases

Gene3Di2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR012850 A-amylase_bs_C
IPR013775 A-amylase_pln
IPR006046 Alpha_amylase
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF07821 Alpha-amyl_C2, 1 hit
PF00128 Alpha-amylase, 1 hit
PIRSFiPIRSF001028 Alph-amls_plant, 1 hit
PRINTSiPR00110 ALPHAAMYLASE
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SM00810 Alpha-amyl_C2, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit

Sequencei

Sequence statusi: Complete.

Q40015-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANKHLSLSL FLVLLGLSAS LASGQVLFQG FNSESWKHNG GWYNFLMGKV
60 70 80 90 100
DDIAAAGITH VWLPPASQSV AEQGYMPGRL YDLDASKYGN KAQLKSLIGA
110 120 130 140 150
LHGKGVKAIA DIVINHRTAE HKDGRGIYCI FEGDTPDARL DWGPHMICRD
160 170 180 190 200
DRPYADGTGN PDTGADFGAA PDIDHLNLRV QKELVEWLNW LKADHRLDGW
210 220 230 240 250
RFDFAKGYSA DVAKIYIDRS EPSFAVAEIW TSLAYGGDGK PNLNQDQHRQ
260 270 280 290 300
ELVNWVDKVG GKGPATTFDF TTKGILNVAV EGELWRLRGT DGKAPGMIGW
310 320 330 340 350
WPAKAVTFVD NHDTGSTQHM WPFPSDRVMQ GYAYILTHPG TPCIFYDHFF
360 370 380 390 400
DWGLKEEIDR LVSVRTRHGI HNESKLQIIE ADADLYLAEI DGKVIVKLGP
410 420
RYDVGNLIPG GFKVAAHGND YAVWQKI
Length:427
Mass (Da):47,403
Last modified:November 1, 1996 - v1
Checksum:iD21BA12EAE5F3534
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17126 mRNA Translation: AAA32925.1
UniGeneiHv.66

Similar proteinsi

Entry informationi

Entry nameiQ40015_HORVU
AccessioniPrimary (citable) accession number: Q40015
Entry historyiIntegrated into UniProtKB/TrEMBL: November 1, 1996
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

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