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Protein

Malate dehydrogenase

Gene

mdh

Organism
Dehalococcoides mccartyi (strain CBDB1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei32 – 321NADUniRule annotation
Binding sitei81 – 811SubstrateUniRule annotation
Binding sitei87 – 871SubstrateUniRule annotation
Binding sitei94 – 941NADUniRule annotation
Binding sitei119 – 1191SubstrateUniRule annotation
Binding sitei150 – 1501SubstrateUniRule annotation
Active sitei174 – 1741Proton acceptorUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi8 – 136NADUniRule annotation
Nucleotide bindingi117 – 1193NADUniRule annotation

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: InterPro
  2. malate metabolic process Source: InterPro
  3. tricarboxylic acid cycle Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciDSP255470:GJXW-345-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
Gene namesi
Name:mdhUniRule annotation
Ordered Locus Names:cbdbA409
OrganismiDehalococcoides mccartyi (strain CBDB1)
Taxonomic identifieri255470 [NCBI]
Taxonomic lineageiBacteriaChloroflexiDehalococcoidiaDehalococcoidalesDehalococcoidaceaeDehalococcoides
ProteomesiUP000000433: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 307307Malate dehydrogenasePRO_0000241946Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi255470.cbdb_A409.

Structurei

3D structure databases

ProteinModelPortaliQ3ZZJ7.
SMRiQ3ZZJ7. Positions 3-305.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 3 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0039.
KOiK00024.
OMAiDGQENAR.
OrthoDBiEOG6091FG.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00487. Malate_dehydrog_3.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01763. MalateDH_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3ZZJ7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPKISVIGAG NVGATLAQRL IEKDFADVVM LDVVEGIPQG KALDISQSAS
60 70 80 90 100
VLGFRHTITG SNDYAQTAGS EIVVITAGIA RKPGMTREEL LAINQKIMTD
110 120 130 140 150
VVSNCLKYSP EATLVVVSNP VDTMTYLAWK LSGLPRKRVV GLSGVLDGGR
160 170 180 190 200
LATFVARELG VNPSAVSPCV MGEHGGSMVV MSRFTLVNGK PLSELVSPEK
210 220 230 240 250
ADELAKRAVN GGAEIVAFLK TGSAFYAPSA SVAAMVEAIF LGSGKVMNCA
260 270 280 290 300
AVLDGEYGLR NIVLGVPVKL GKGGIKEIIT LPLDGQENAR LQASAEMVKV

QIASLSL
Length:307
Mass (Da):32,023
Last modified:September 27, 2005 - v1
Checksum:iCA4EDE5D03EC51BD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ965256 Genomic DNA. Translation: CAI82616.1.
RefSeqiYP_307532.1. NC_007356.1.

Genome annotation databases

EnsemblBacteriaiCAI82616; CAI82616; cbdbA409.
GeneIDi3623745.
KEGGideh:cbdb_A409.
PATRICi21610989. VBIDehSp125902_0340.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ965256 Genomic DNA. Translation: CAI82616.1.
RefSeqiYP_307532.1. NC_007356.1.

3D structure databases

ProteinModelPortaliQ3ZZJ7.
SMRiQ3ZZJ7. Positions 3-305.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi255470.cbdb_A409.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAI82616; CAI82616; cbdbA409.
GeneIDi3623745.
KEGGideh:cbdb_A409.
PATRICi21610989. VBIDehSp125902_0340.

Phylogenomic databases

eggNOGiCOG0039.
KOiK00024.
OMAiDGQENAR.
OrthoDBiEOG6091FG.

Enzyme and pathway databases

BioCyciDSP255470:GJXW-345-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00487. Malate_dehydrog_3.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01763. MalateDH_bact. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the chlorinated compound-respiring bacterium Dehalococcoides species strain CBDB1."
    Kube M., Beck A., Zinder S.H., Kuhl H., Reinhardt R., Adrian L.
    Nat. Biotechnol. 23:1269-1273(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CBDB1.

Entry informationi

Entry nameiMDH_DEHMC
AccessioniPrimary (citable) accession number: Q3ZZJ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: September 27, 2005
Last modified: February 4, 2015
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.