Reviewed, UniProtKB/Swiss-Prot Q3ZZG9 (SYV_DEHSC)

Names and origin

Protein names

Recommended name:
    Valyl-tRNA synthetase
    EC=6.1.1.9
Alternative name(s):
    Valine--tRNA ligase
      Short name=ValRS

Gene names

Name:	valS
Ordered Locus Names:	cbdbA384

Organism

Dehalococcoides sp. (strain CBDB1) [Complete proteome] [HAMAP]

Taxonomic identifier

255470 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Dehalococcoidetes › Dehalococcoides

Protein attributes

Sequence length	880 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a "posttransfer" editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner By similarity.
Catalytic activity	ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val). HAMAP MF_02004
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site By similarity. The C-terminal coiled-coil domain is crucial for aminoacylation activity By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	valyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP valine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 880	880	Valyl-tRNA synthetase HAMAP MF_02004		PRO_0000224472
Regions
	Coiled coil	815 – 854	40	Potential
	Motif	51 – 61	11	"HIGH" region HAMAP MF_02004
	Motif	529 – 533	5	"KMSKS" region HAMAP MF_02004
Sites
	Binding site	532	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3ZZG9-1 [UniParc]. Last modified September 27, 2005. Version 1. Checksum: E0A6F24298F69053	FASTA	880	100,189
	10 20 30 40 50 60 MAQCSDLPEM AKAYEAAEVE KKWYQYWMEK SYFKPNPNSD KKPFVIIMPP PNVTGELHLG 70 80 90 100 110 120 HALTATLEDI MIRWHRMQGE PTLWLPGVDH AGIAAQVVVE RELAKQGKTR QQLGRELFLE 130 140 150 160 170 180 KMWEWVNPCR EKIRHQHMRL GASCDWDRET FTLDAGPVKA VREIFTNLYE KGLIYKGERI 190 200 210 220 230 240 INWCPRCGTA VSDLEVDHKD LAGHIWHLRY PLEDGSGFVT VATTRPETMQ GDTAVAIHPD 250 260 270 280 290 300 DTRYAGMVGK NVVLPIMNRR IPVIADEAVD MAFGTGAVKV TPAHDPNDFE MGLRHNLPMI 310 320 330 340 350 360 TIQNRDTTMN ENAGPCSGMT AKACREYVVS EMKSLGLLLR IEDYIHSVGH CQRCSAVIEP 370 380 390 400 410 420 MVSKQWFVKM EPLAKPALEA VNSGRIQILP ERFNKVYQNW MENIRDWCIS RQLWWGHRIP 430 440 450 460 470 480 VWYCPCGEMI VAKVDPTVCP KCGGTELEQD PDVLDTWFSS GLWPHSTLGW PDQTEDLKRF 490 500 510 520 530 540 YPGTVMETAY DIIFFWVARM IVMGMEDMNE VPFRTVYLHG LIRDDKGEKM SKTKGNVIDP 550 560 570 580 590 600 LKVIDQYGTD ALRFAVTFGT SPGNDSKLGQ TKLEAARNFA NKLWNASRFV IMNLGEAKEL 610 620 630 640 650 660 TPEAELPLED RWIISRMNRV TADVTRLMEE FQFGEAQRVL QDFIWGEFCD WYIELAKVRL 670 680 690 700 710 720 RDEASVSPRP VLVRVLSSIL RLLHPYMPFI TEELWSYLRP YLPESLRETD IIVAPYPAAD 730 740 750 760 770 780 KTCFDEQAES VMGSLVEIVR SLRNLRAEHN VEISRYIQAN IYAGDMASVL GNYLGAVETL 790 800 810 820 830 840 SRARPVNILP GHYSGASTAT EVVLVLTGIE VVVPMSTMVD LEVEAKRVKA EISELEIQIE 850 860 870 880 RLSTRLSDEQ FLAKAPQAVV DKERIKLEGY IEKVSRLKSA

References

[1]	"Genome sequence of the chlorinated compound-respiring bacterium Dehalococcoides species strain CBDB1." Kube M., Beck A., Zinder S.H., Kuhl H., Reinhardt R., Adrian L. Nat. Biotechnol. 23:1269-1273(2005) [PubMed: 16116419] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ965256 Genomic DNA. Translation: CAI82595.1.
RefSeq	YP_307511.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3623827.
GenomeReviews	Gene locus cbdbA384 in contig AJ965256_GR.
KEGG	deh:cbdb_A384.
NMPDR	fig|255470.3.peg.768.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3ZZG9.
OMA	Q3ZZG9. TDQWYVS.
Enzyme and pathway databases
BioCyc	DSP255470:CBDBA384-MON.
Family and domain databases
HAMAP	MF_02004. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR014729. Rossmann-like_a/b/a_fold. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR002303. Val-tRNA_synth_Ia. IPR019754. Val-tRNA_synth_Ia_N. IPR019499. Val-tRNA_synth_Ia_tRNA-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11946:SF5. tRNA-synt_val. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. PF10458. Val_tRNA-synt_C. 1 hit. [Graphical view]
PRINTS	PR00986. TRNASYNTHVAL.
TIGRFAMs	TIGR00422. valS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYV_DEHSC

Accession

Primary (citable) accession number: Q3ZZG9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	September 27, 2005
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)