Q3ZYP4 (SYE_DEHSC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	cbdbA1316

Organism

Dehalococcoides sp. (strain CBDB1) [Complete proteome] [HAMAP]

Taxonomic identifier

255470 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Dehalococcoidetes › Dehalococcoides

Protein attributes

Sequence length	487 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 487	487	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_0000237358
Regions
	Motif	11 – 21	11	"HIGH" region HAMAP MF_00022_B
	Motif	245 – 249	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Metal binding	108	1	Zinc By similarity
	Metal binding	110	1	Zinc By similarity
	Metal binding	135	1	Zinc By similarity
	Metal binding	137	1	Zinc By similarity
	Binding site	248	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3ZYP4 [UniParc]. Last modified September 27, 2005. Version 1. Checksum: 3D04551A2A9DA0D4	FASTA	487	55,527
	10 20 30 40 50 60 MTNEVRVRYA PSPTGYPHLG NIRTAMFNWL FARHNGGKFI VRIEDTDRER YVEGAVESIL 70 80 90 100 110 120 ESLNWLGLDW DEGPDKGGDY GPYYQSERLP LYRKAAEKLV AEGKAYYCHC SSEKLDKMRE 130 140 150 160 170 180 DQIARKEPPG YDRCCRDMGL GQKEGAVIRF KIPLDGQTAF TDLIRGEVTF DNAKQDDFVI 190 200 210 220 230 240 LKSDGFPTYH LASVVDDHAM QISHVLRAEE WLPSTPKHLM LYKALGYTPP LYAHLPMILG 250 260 270 280 290 300 PDRSKLSKRH GATSTIEYKQ AGYLPETMVN FLSLLGWAYD DKTELFSREQ LIEYFCLEKV 310 320 330 340 350 360 SKTAAIFNYE KLDWMNGMYI RTLSAQDLAC RAMPFLEKDV RIAASGHLNL DYTVKVMPLI 370 380 390 400 410 420 QERAKKLNEL AELCWFIYSD DISYDPALLI DKKLTKETSL SALKAANARL EALPNFDAAS 430 440 450 460 470 480 MEEHIRPLAA ELELKPGQLF GMLRTASTGQ QVAPPLFQTM EVLGRQRCLW RIAMAIARLS EMPFQRS

References

[1]

"Genome sequence of the chlorinated compound-respiring bacterium Dehalococcoides species strain CBDB1." Kube M., Beck A., Zinder S.H., Kuhl H., Reinhardt R., Adrian L. Nat. Biotechnol. 23:1269-1273(2005) [PubMed: 16116419] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CBDB1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ965256 Genomic DNA. Translation: CAI83372.1.
RefSeq	YP_308288.1. NC_007356.1.
3D structure databases
ProteinModelPortal	Q3ZYP4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3ZYP4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3623605.
GenomeReviews	Gene locus cbdbA1316 in contig AJ965256_GR.
KEGG	deh:cbdb_A1316.
NMPDR	fig|255470.3.peg.1204.
PATRIC	21612575. VBIDehSp125902_1104.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	MAHIPLI.
PhylomeDB	Q3ZYP4.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	DSP255470:CBDBA1316-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_DEHSC

Accession

Primary (citable) accession number: Q3ZYP4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	September 27, 2005
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program