Q3ZYH8 (RNPH_DEHSC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease PH
Short name=RNase PH
EC=2.7.7.56

Alternative name(s):
tRNA nucleotidyltransferase

Gene names

Name:	rph
Ordered Locus Names:	cbdbA1225

Organism

Dehalococcoides sp. (strain CBDB1) [Complete proteome] [HAMAP]

Taxonomic identifier

255470 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Dehalococcoidetes › Dehalococcoides

Protein attributes

Sequence length	241 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity. HAMAP MF_00564
Catalytic activity	tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP MF_00564
Sequence similarities	Belongs to the RNase PH family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	tRNA binding Inferred from electronic annotation. Source: InterPro tRNA nucleotidyltransferase activity Inferred from electronic annotation. Source: EC tRNA-specific ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 241	241	Ribonuclease PH HAMAP MF_00564		PRO_1000061132

Sequences

	Sequence		Length	Mass (Da)
	Q3ZYH8 [UniParc]. Last modified September 27, 2005. Version 1. Checksum: E7EDE4931AA9EDEC	FASTA	241	26,556
	10 20 30 40 50 60 MQRGDGRNFN QLRPITITPG FQSFAEGSVL IEQGKTRVIC SVSMEDRVPQ FLRNSGTGWV 70 80 90 100 110 120 TAEYSMLPRS TVTRTQRDSS AGKISGRSQE IQRLIGRSLR SCVDMAALGE RSFIVDCDVI 130 140 150 160 170 180 QADAGTRTAS ITGSYIALYL AFKKMVDMGI LSKMPFTSQV AAVSVSIFKG NIVLDPCYDE 190 200 210 220 230 240 DFQAEVDFNL VMNDRGEFVE IQGTAEGKAF SRDTLDQVLK LGEAGIRQLF DIQKGITQPQ L

References

[1]

"Genome sequence of the chlorinated compound-respiring bacterium Dehalococcoides species strain CBDB1." Kube M., Beck A., Zinder S.H., Kuhl H., Reinhardt R., Adrian L. Nat. Biotechnol. 23:1269-1273(2005) [PubMed: 16116419] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CBDB1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ965256 Genomic DNA. Translation: CAI83296.1.
RefSeq	YP_308212.1. NC_007356.1.
3D structure databases
HSSP	HSSP built from PDB template 2BA1 based on UniProtKB O29756.
ProteinModelPortal	Q3ZYH8.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3ZYH8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3623385.
GenomeReviews	Gene locus cbdbA1225 in contig AJ965256_GR.
KEGG	deh:cbdb_A1225.
NMPDR	fig|255470.3.peg.1003.
PATRIC	21612417. VBIDehSp125902_1026.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0689.
HOGENOM	HBG737187.
OMA	MLPRATG.
PhylomeDB	Q3ZYH8.
ProtClustDB	PRK00173.
Enzyme and pathway databases
BioCyc	DSP255470:CBDBA1225-MONOMER.
Family and domain databases
HAMAP	MF_00564. RNase_PH. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR020568. Ribosomal_S5_D2-typ_fold. IPR002381. RNase_PH_bac-type. IPR018336. RNase_PH_CS. [Graphical view]
KO	K00989.
Pfam	PF01138. RNase_PH. 1 hit. PF03725. RNase_PH_C. 1 hit. [Graphical view]
SUPFAM	SSF55666. 3_ExoRNase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR01966. RNasePH. 1 hit.
PROSITE	PS01277. RIBONUCLEASE_PH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPH_DEHSC

Accession

Primary (citable) accession number: Q3ZYH8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	September 27, 2005
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program