Reviewed, UniProtKB/Swiss-Prot Q3ZYG4 (ARGJ_DEHSC)

Names and origin

Protein names

Recommended name:
    Arginine biosynthesis bifunctional protein argJ
Cleaved into the following 2 chains:
    1- Recommended name:
            Arginine biosynthesis bifunctional protein argJ alpha chain
    2- Recommended name:
            Arginine biosynthesis bifunctional protein argJ beta chain
Including the following 2 domains:
    1- Recommended name:
            Glutamate N-acetyltransferase
              EC=2.3.1.35
        Alternative name(s):
            Ornithine acetyltransferase
              Short name=OATase
            Ornithine transacetylase
    2- Recommended name:
            Amino-acid acetyltransferase
              EC=2.3.1.1
        Alternative name(s):
            N-acetylglutamate synthase
              Short name=AGS

Gene names

Name:	argJ
Ordered Locus Names:	cbdbA1179

Organism

Dehalococcoides sp. (strain CBDB1) [Complete proteome] [HAMAP]

Taxonomic identifier

255470 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Dehalococcoidetes › Dehalococcoides

Protein attributes

Sequence length	405 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of acetylglutamate from glutamate and acetyl-CoA, and of ornithine by transacetylation between acetylornithine and glutamate By similarity. HAMAP MF_01106
Catalytic activity	N(2)-acetyl-L-ornithine + L-glutamate = L-ornithine + N-acetyl-L-glutamate. HAMAP MF_01106 Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate. HAMAP MF_01106
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic): step 1/1. HAMAP MF_01106 Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4. HAMAP MF_01106
Subunit structure	Heterotetramer of two alpha and two beta chains By similarity. HAMAP MF_01106
Subcellular location	Cytoplasm Probable HAMAP MF_01106.
Miscellaneous	Some bacteria possess a monofunctional argJ, i.e., capable of catalyzing only the fifth step of the arginine biosynthetic pathway. HAMAP MF_01106
Sequence similarities	Belongs to the argJ family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetyl-CoA:L-glutamate N-acetyltransferase activity Inferred from electronic annotation. Source: HAMAP glutamate N-acetyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 191	191	Arginine biosynthesis bifunctional protein argJ alpha chain By similarity		PRO_0000227226
	Chain	192 – 405	214	Arginine biosynthesis bifunctional protein argJ beta chain By similarity		PRO_0000227227
Sites
	Site	191 – 192	2	Cleavage; by autolysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3ZYG4-1 [UniParc]. Last modified September 27, 2005. Version 1. Checksum: 9E5A0F59BDB4380C	FASTA	405	43,153
	10 20 30 40 50 60 MKSSIDILPD GSITTPKGFK AGAIYAGIKK KSKNNLDLAI LYSDTPCIAA GIFTTNKFRA 70 80 90 100 110 120 APVYISEHNL GFTDNRAIVV NSGCANAGTG EAGMADAIEM VKATAESLNM QPKDVLIAST 130 140 150 160 170 180 GVIGHRLPID KIKENVRLIG LSQRNGHEFA RAIMTTDTRS KEIAVQVNIE GFQFYIAGAA 190 200 210 220 230 240 KGAGMIHPNM ATMLGFITTD ASVSKEFLQF ALKEAADVSF NMITVDGDTS TNDSLFMLSN 250 260 270 280 290 300 GQAENPTFAG NTEYSLVFQQ ALTIVCQNLA KSIARDGEGA KRLIEIQVEG AANLEDARLI 310 320 330 340 350 360 ARVIAGSPLV KTAVHGADPN WGRILAAAGR AGADFDIDMV DLYLGESPVL LKGARTGVDE 370 380 390 400 KELSSWLRQV EVIIKLNLNL GQGKAAAWGC DLSAEYVKIN ADYTT

References

[1]	"Genome sequence of the chlorinated compound-respiring bacterium Dehalococcoides species strain CBDB1." Kube M., Beck A., Zinder S.H., Kuhl H., Reinhardt R., Adrian L. Nat. Biotechnol. 23:1269-1273(2005) [PubMed: 16116419] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ965256 Genomic DNA. Translation: CAI83263.1.
RefSeq	YP_308179.1.
3D structure databases
SMR	Q3ZYG4. Positions 7-191, 15-402, 192-403.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3ZYG4.
Protein family/group databases
MEROPS	T05.001.
Genome annotation databases
GeneID	3623274.
GenomeReviews	Gene locus cbdbA1179 in contig AJ965256_GR.
KEGG	deh:cbdb_A1179.
NMPDR	fig|255470.3.peg.909.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1364.
HOGENOM	HBG284202.
OMA	QNRFCAA.
PhylomeDB	Q3ZYG4.
Enzyme and pathway databases
BioCyc	DSP255470:CBDBA1179-MONOMER.
Family and domain databases
HAMAP	MF_01106. ArgJ. [Tree]
InterPro	IPR002813. Arg_biosynth_ArgJ. IPR016117. Pept_S58_DmpA/Arg_biosyn_ArgJ. [Graphical view]
PANTHER	PTHR23100. ArgJ. 1 hit.
Pfam	PF01960. ArgJ. 1 hit. [Graphical view]
TIGRFAMs	TIGR00120. ArgJ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARGJ_DEHSC

Accession

Primary (citable) accession number: Q3ZYG4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	September 27, 2005
Last modified:	February 9, 2010
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)