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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Dehalococcoides mccartyi (strain CBDB1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei631 – 6311ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciDSP255470:GJXW-878-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:cbdbA1012
OrganismiDehalococcoides mccartyi (strain CBDB1)
Taxonomic identifieri255470 [NCBI]
Taxonomic lineageiBacteriaChloroflexiDehalococcoidiaDehalococcoidalesDehalococcoidaceaeDehalococcoides

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10141014Isoleucine--tRNA ligasePRO_0000098541Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi255470.cbdb_A1012.

Structurei

3D structure databases

ProteinModelPortaliQ3ZY20.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi48 – 5811"HIGH" regionAdd
BLAST
Motifi628 – 6325"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiIPLPIWR.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3ZY20-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKAVNPRQN FPQMEEEILK IWQDKGIFKK SIENRRDGKR FTLYEGPPTA
60 70 80 90 100
NGRPGIHHVL SRVFKDVIPR YKVMKGYYAP RIGGWDTHGL PVELEVEKEL
110 120 130 140 150
GFTSKNDIEK YGIAEFNTRC RSSVFKYVSE WNKLTERIAY WVDLDNAYIT
160 170 180 190 200
MDNKYIESGW WALKQMWDKG LVYQGHRVTP HCPRCGTSLS SHEVAQGYKD
210 220 230 240 250
NTEDPSVFVK FEINHESLAQ TGLGQKWVNP SDKPLYLLAW TTTPWTLPAN
260 270 280 290 300
TALAVSATDE YAILDMADYY MVLAKPRLSS LKLTENPIVG ECLGSDLKGL
310 320 330 340 350
TYKPLFDPRE FGISVKNMQD NSEIDALEPL SYPVITTSYV SMDDGTGIVH
360 370 380 390 400
TAPAYGELDY ESGVKYGLKF VHHVDLQGRI TGNYPFAGKF VKEADKDISR
410 420 430 440 450
NLKERGLMFR NERMHHTYPF CWRCDSPLIY YAKQSWYIRT TAVRDELIKG
460 470 480 490 500
NQQINWYPEH IKDGRFGDWL ENNIDWAFSR ERYWGTPVPI WRCEKCGQTE
510 520 530 540 550
CVGGIDELKA KPNFKGMQEK LDIHRPYADE WTYDCAKCGG NMKRVTEVMD
560 570 580 590 600
CWYDSGAMPV AQYHYPFEPE SRTIASDGRF PADYICEAVD QTRGWFYSLH
610 620 630 640 650
AISTLIFGRP CYQNVICLGH ILDERGEKMS KSKNNVIQPA AVLDKYGADA
660 670 680 690 700
VRWYFYTAAP PGNARRFSEK LVGEVTRQFL LMLWNIYSFF VTYANIDNFT
710 720 730 740 750
PSEKYLAGEV PELDRWILSE LNQLVLDVDK GLDNYDPTQA GRRIEDFVGY
760 770 780 790 800
LSNWYVRRSR RRFWKSENDA DKLSAYQALY TCLVTLSKLL APFTPFVAEE
810 820 830 840 850
LYQNLVLLVD QSALESVHLT DFPVADKALI DEQLDNEIRL VMKVSSMGRS
860 870 880 890 900
ARSKAALKVR QPLAEVRVVL SSPAERTGLM RLAEQVLEEL NVKALVAEEP
910 920 930 940 950
GTAIPQENYV ASTEGAYTVA VYTGLSPELL AEGSAREIVH RLQTMRKSAE
960 970 980 990 1000
FEIADYINTH YQADEYLESV IRTHAEYIKK ETLSNQIVNG NAPEGAYTES
1010
LDIDGHPLSL WVVR
Length:1,014
Mass (Da):116,166
Last modified:September 27, 2005 - v1
Checksum:iFAC5A5AA9D9830A7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ965256 Genomic DNA. Translation: CAI83123.1.
RefSeqiWP_011309474.1. NC_007356.1.
YP_308039.1. NC_007356.1.

Genome annotation databases

EnsemblBacteriaiCAI83123; CAI83123; cbdbA1012.
KEGGideh:cbdb_A1012.
PATRICi21612063. VBIDehSp125902_0851.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ965256 Genomic DNA. Translation: CAI83123.1.
RefSeqiWP_011309474.1. NC_007356.1.
YP_308039.1. NC_007356.1.

3D structure databases

ProteinModelPortaliQ3ZY20.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi255470.cbdb_A1012.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAI83123; CAI83123; cbdbA1012.
KEGGideh:cbdb_A1012.
PATRICi21612063. VBIDehSp125902_0851.

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiIPLPIWR.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciDSP255470:GJXW-878-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the chlorinated compound-respiring bacterium Dehalococcoides species strain CBDB1."
    Kube M., Beck A., Zinder S.H., Kuhl H., Reinhardt R., Adrian L.
    Nat. Biotechnol. 23:1269-1273(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CBDB1.

Entry informationi

Entry nameiSYI_DEHMC
AccessioniPrimary (citable) accession number: Q3ZY20
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: September 27, 2005
Last modified: June 24, 2015
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.