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Q3ZY20

- SYI_DEHMC

UniProt

Q3ZY20 - SYI_DEHMC

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Dehalococcoides mccartyi (strain CBDB1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (27 Sep 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei631 – 6311ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciDSP255470:GJXW-878-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:cbdbA1012
    OrganismiDehalococcoides mccartyi (strain CBDB1)
    Taxonomic identifieri255470 [NCBI]
    Taxonomic lineageiBacteriaChloroflexiDehalococcoidiaDehalococcoidalesDehalococcoidaceaeDehalococcoides
    ProteomesiUP000000433: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10141014Isoleucine--tRNA ligasePRO_0000098541Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi255470.cbdb_A1012.

    Structurei

    3D structure databases

    ProteinModelPortaliQ3ZY20.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi48 – 5811"HIGH" regionAdd
    BLAST
    Motifi628 – 6325"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    KOiK01870.
    OMAiKWIISEI.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q3ZY20-1 [UniParc]FASTAAdd to Basket

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    MFKAVNPRQN FPQMEEEILK IWQDKGIFKK SIENRRDGKR FTLYEGPPTA     50
    NGRPGIHHVL SRVFKDVIPR YKVMKGYYAP RIGGWDTHGL PVELEVEKEL 100
    GFTSKNDIEK YGIAEFNTRC RSSVFKYVSE WNKLTERIAY WVDLDNAYIT 150
    MDNKYIESGW WALKQMWDKG LVYQGHRVTP HCPRCGTSLS SHEVAQGYKD 200
    NTEDPSVFVK FEINHESLAQ TGLGQKWVNP SDKPLYLLAW TTTPWTLPAN 250
    TALAVSATDE YAILDMADYY MVLAKPRLSS LKLTENPIVG ECLGSDLKGL 300
    TYKPLFDPRE FGISVKNMQD NSEIDALEPL SYPVITTSYV SMDDGTGIVH 350
    TAPAYGELDY ESGVKYGLKF VHHVDLQGRI TGNYPFAGKF VKEADKDISR 400
    NLKERGLMFR NERMHHTYPF CWRCDSPLIY YAKQSWYIRT TAVRDELIKG 450
    NQQINWYPEH IKDGRFGDWL ENNIDWAFSR ERYWGTPVPI WRCEKCGQTE 500
    CVGGIDELKA KPNFKGMQEK LDIHRPYADE WTYDCAKCGG NMKRVTEVMD 550
    CWYDSGAMPV AQYHYPFEPE SRTIASDGRF PADYICEAVD QTRGWFYSLH 600
    AISTLIFGRP CYQNVICLGH ILDERGEKMS KSKNNVIQPA AVLDKYGADA 650
    VRWYFYTAAP PGNARRFSEK LVGEVTRQFL LMLWNIYSFF VTYANIDNFT 700
    PSEKYLAGEV PELDRWILSE LNQLVLDVDK GLDNYDPTQA GRRIEDFVGY 750
    LSNWYVRRSR RRFWKSENDA DKLSAYQALY TCLVTLSKLL APFTPFVAEE 800
    LYQNLVLLVD QSALESVHLT DFPVADKALI DEQLDNEIRL VMKVSSMGRS 850
    ARSKAALKVR QPLAEVRVVL SSPAERTGLM RLAEQVLEEL NVKALVAEEP 900
    GTAIPQENYV ASTEGAYTVA VYTGLSPELL AEGSAREIVH RLQTMRKSAE 950
    FEIADYINTH YQADEYLESV IRTHAEYIKK ETLSNQIVNG NAPEGAYTES 1000
    LDIDGHPLSL WVVR 1014
    Length:1,014
    Mass (Da):116,166
    Last modified:September 27, 2005 - v1
    Checksum:iFAC5A5AA9D9830A7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ965256 Genomic DNA. Translation: CAI83123.1.
    RefSeqiYP_308039.1. NC_007356.1.

    Genome annotation databases

    EnsemblBacteriaiCAI83123; CAI83123; cbdbA1012.
    GeneIDi3623543.
    KEGGideh:cbdb_A1012.
    PATRICi21612063. VBIDehSp125902_0851.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ965256 Genomic DNA. Translation: CAI83123.1 .
    RefSeqi YP_308039.1. NC_007356.1.

    3D structure databases

    ProteinModelPortali Q3ZY20.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 255470.cbdb_A1012.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAI83123 ; CAI83123 ; cbdbA1012 .
    GeneIDi 3623543.
    KEGGi deh:cbdb_A1012.
    PATRICi 21612063. VBIDehSp125902_0851.

    Phylogenomic databases

    eggNOGi COG0060.
    KOi K01870.
    OMAi KWIISEI.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci DSP255470:GJXW-878-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the chlorinated compound-respiring bacterium Dehalococcoides species strain CBDB1."
      Kube M., Beck A., Zinder S.H., Kuhl H., Reinhardt R., Adrian L.
      Nat. Biotechnol. 23:1269-1273(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CBDB1.

    Entry informationi

    Entry nameiSYI_DEHMC
    AccessioniPrimary (citable) accession number: Q3ZY20
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: September 27, 2005
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3