Q3ZXC2 (DXS_DEHSC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose-5-phosphate synthase
EC=2.2.1.7

Alternative name(s):
1-deoxyxylulose-5-phosphate synthase
Short name=DXP synthase
Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	cbdbA720

Organism

Dehalococcoides sp. (strain CBDB1) [Complete proteome] [HAMAP]

Taxonomic identifier

255470 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Dehalococcoidetes › Dehalococcoides

Protein attributes

Sequence length	647 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 647	647	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_0000256409

Sequences

	Sequence		Length	Mass (Da)
	Q3ZXC2 [UniParc]. Last modified September 27, 2005. Version 1. Checksum: F4C1160B614CABC7	FASTA	647	70,076
	10 20 30 40 50 60 MNTGLEISIL HNLEMSKLLD TINSPSDLKK LSLDELRELA VQIREELVNR VTLNGGHLAS 70 80 90 100 110 120 SLGVVELTIA LHRVFESPKD KIIWDVGHQS YAHKLLTGRR EQFATLRQHG GLSGFTCRDE 130 140 150 160 170 180 SPHDPFGAGH ASTSISAGLG MAVARDLAKE DYSVISVIGD GAISGGMSFE AINNAGHLHT 190 200 210 220 230 240 KFIVILNDNG MAISPSTGAL SKFLNNVRFD PRFEFAKRNA KQTITNMPFG KAVWAFTKSI 250 260 270 280 290 300 KRKFEKSMLP GSLWEELGFI YLGPVDGHNI RELEAALKRA KDFESKPVLI HMITKKGKGY 310 320 330 340 350 360 DDAEADAVKY HGISPKSGGL KSSHGLSYSQ VFGQTLHKIM SQNPQVVAIT AAMTDGCGLG 370 380 390 400 410 420 EIAAAFPDRV FDVGICEQHA VTFAAGMATQ GYIPVVVIYS TFLQRGFDQI IHDVCLQKLP 430 440 450 460 470 480 VVFAIDRGGI VGDDGKTHQG IFDLSFMSLI PDMVVSAPSD ENDLQHLIYT AVNSGKPFAL 490 500 510 520 530 540 RYPRGFGEGA EIESSLHNIP IGQNEILVNG SDVAILATGK SVAFAKDALE ILTESGIKPT 550 560 570 580 590 600 LVNNRYISPL DSELVLKIAQ SHKYLVTVEE NVISGGLGSR INTLLAEAGL VNKIKIANIG 610 620 630 640 IPDKFVEHGN QSLLRAKYGL DGKGIAQRVL SLVGNPNEMK HPQIICP

References

[1]

"Genome sequence of the chlorinated compound-respiring bacterium Dehalococcoides species strain CBDB1." Kube M., Beck A., Zinder S.H., Kuhl H., Reinhardt R., Adrian L. Nat. Biotechnol. 23:1269-1273(2005) [PubMed: 16116419] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CBDB1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ965256 Genomic DNA. Translation: CAI82881.1.
RefSeq	YP_307797.1. NC_007356.1.
3D structure databases
ProteinModelPortal	Q3ZXC2.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3ZXC2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3623188.
GenomeReviews	Gene locus cbdbA720 in contig AJ965256_GR.
KEGG	deh:cbdb_A720.
NMPDR	fig|255470.3.peg.1455.
PATRIC	21611541. VBIDehSp125902_0609.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1154.
HOGENOM	HBG571647.
OMA	DPILYHG.
PhylomeDB	Q3ZXC2.
ProtClustDB	PRK05444.
Enzyme and pathway databases
BioCyc	DSP255470:CBDBA720-MONOMER.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR001017. DH_E1. IPR005477. Dxylulose-5-P_synthase. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K01662.
Pfam	PF00676. E1_dh. 1 hit. PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00204. Dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_DEHSC

Accession

Primary (citable) accession number: Q3ZXC2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	September 27, 2005
Last modified:	January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program