Q3ZX75 (Q3ZX75_DEHSC) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518

Gene names

Name:	topA EMBL CAI82839.1
Ordered Locus Names:	cbdbA674

Organism

Dehalococcoides sp. (strain CBDB1) [Complete proteome] [HAMAP]

Taxonomic identifier

255470 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Dehalococcoidetes › Dehalococcoides

Protein attributes

Sequence length	703 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 RuleBase RU000518 Nucleotide-binding
Molecular function	Isomerase Topoisomerase RuleBase RU000518 SAAS SAAS005733
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q3ZX75 [UniParc]. Last modified September 27, 2005. Version 1. Checksum: 744369722197C7A1	FASTA	703	79,820
	10 20 30 40 50 60 MKEKLVIVES PAKARTISKM LGKDFNIMAT MGHIRDLPKS TLGVDVENNF TPKYVSLKAK 70 80 90 100 110 120 AKVIKELKEA VKNSKAIYLA TDPDREGEAI AWHISEVTKA NLSNPKRVVF HEITKEAIDK 130 140 150 160 170 180 AFKNTRELDM DLVNAQQARR VLDRLVGYKL SPLLWRKVQR GLSAGRVQSV SLKIIVDRER 190 200 210 220 230 240 EIEKFVSTEY WNIEALLSKK VKSAAFKATL AGYVTKGKLE IHNESEADQI KKELEVSDYQ 250 260 270 280 290 300 VLKIKRKSNL RQSPAPFITS TLQQEAWRKL RFSAKQTMVI AQQLYEGLNV EGEGEVGLIT 310 320 330 340 350 360 YMRTDSTNVA RTAVAETRDY ITEKYGPAYL PKQARVFSSK VKGAQEAHEA IRPTRINRTP 370 380 390 400 410 420 DFIKKSLSAD QFKLYQLIWQ RMIASQMSPA VFDNTTIDIE AKHQPSKTRY LLRTTSSVNT 430 440 450 460 470 480 FPGFTIVYIE GKDEDEEKEQ SSLPPMEEGE ELKLKSLDAT QHFTQPPPRY TEATLIKALE 490 500 510 520 530 540 QNGIGRPSTY APTISTIQER EYITKLKGSL KPSELGMLVN DLLVEHFPDI IGIDFTAAME 550 560 570 580 590 600 AELDKIATEK LDWVPIIRRF YEPFIKEIEA ASEKIEKIKP PEEELDELCP ECNAKLVIKR 610 620 630 640 650 660 GRFGKFIACS RYSKEDDGCR YTRSYQIKTG AKCPDCNSDI VEKYSKKGKI FYGCAGYPNC 670 680 690 700 KFVSFYKPLT KPCPECGALM TKSGQNWAKC TKCGKRKKLE DES

References

[1]	"Genome sequence of the chlorinated compound-respiring bacterium Dehalococcoides species strain CBDB1." Kube M., Beck A., Zinder S.H., Kuhl H., Reinhardt R., Adrian L. Nat. Biotechnol. 23:1269-1273(2005) [PubMed: 16116419] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ965256 Genomic DNA. Translation: CAI82839.1.
RefSeq	YP_307755.1. NC_007356.1.
3D structure databases
ProteinModelPortal	Q3ZX75.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3ZX75.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3624027.
GenomeReviews	Gene locus cbdbA674 in contig AJ965256_GR.
KEGG	deh:cbdb_A674.
NMPDR	fig|255470.3.peg.1360.
PATRIC	21611449. VBIDehSp125902_0569.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0550.
HOGENOM	HBG512935.
OMA	EEYWSID.
PhylomeDB	Q3ZX75.
ProtClustDB	PRK05582.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 2 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q3ZX75_DEHSC

Accession

Primary (citable) accession number: Q3ZX75

Entry history

Integrated into UniProtKB/TrEMBL:	September 27, 2005
Last sequence update:	September 27, 2005
Last modified:	December 14, 2011
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)