ID   FTHS_DEHMC              Reviewed;         597 AA.
AC   Q3ZX40;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   27-SEP-2005, sequence version 1.
DT   27-MAR-2024, entry version 95.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543};
GN   OrderedLocusNames=cbdbA660;
OS   Dehalococcoides mccartyi (strain CBDB1).
OC   Bacteria; Chloroflexota; Dehalococcoidia; Dehalococcoidales;
OC   Dehalococcoidaceae; Dehalococcoides.
OX   NCBI_TaxID=255470;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CBDB1;
RX   PubMed=16116419; DOI=10.1038/nbt1131;
RA   Kube M., Beck A., Zinder S.H., Kuhl H., Reinhardt R., Adrian L.;
RT   "Genome sequence of the chlorinated compound-respiring bacterium
RT   Dehalococcoides species strain CBDB1.";
RL   Nat. Biotechnol. 23:1269-1273(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6R)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:195366, ChEBI:CHEBI:456216;
CC         EC=6.3.4.3; Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR   EMBL; AJ965256; CAI82827.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q3ZX40; -.
DR   SMR; Q3ZX40; -.
DR   KEGG; deh:cbdbA660; -.
DR   HOGENOM; CLU_003601_3_3_0; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000000433; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.30.1510.10; Domain 2, N(10)-formyltetrahydrofolate synthetase; 1.
DR   Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
DR   PROSITE; PS00722; FTHFS_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism.
FT   CHAIN           1..597
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_0000199345"
FT   BINDING         84..91
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   597 AA;  64493 MW;  775FDB2876B52CB5 CRC64;
     MAVNDREFHL KELDPVLMKD WEIAEKAEDF LKPVKVLAME LGLTEDEIIP HGKYIAKVDF
     AGVLARLKDR PNGKYIDVTA ITPTPLGEGK STTTMGLVQG LGKLGKKVTG AIRQPSSGPT
     FNIKGSAAGG GLSQCLPLSP FSLGLTGDID AVTNSHNLAM VALQARLQHE ANNTDEFLSS
     RHLTRLDIDP SRVEMKWAMD FCAQSLREII MGIGGKTDGY RMPSGFGISV SSEVMAILSV
     FTSLSDLRER MGKIIVAYRK NDEPVTTADL EVDGAMTALL LRAVNPNLLQ TIEGQPVFVH
     AGPFANIAIG QSSIVADRLA LKLADYHVTE SGFGADIGFE KFWNIKCRLS GLKPDCAVIV
     VTARALKMHG GGPKVTPGAP LDPAYTTPNT KLVEKGCQNM LAHIQTVKTA GINPVVCINH
     FAADTHEEID IIRRTAEQAE ARVAVSHHWA KGGDGATELA EAVTDACNEP NNFHFLYPEN
     MPLKKRIETI ARKVYGANGV SYTPIAMEKL ARIESMGDTQ FMPTCMVKTH LSLSHDPALK
     GRPSGFTLPI RDILTYMGAG LVVPVAGDIK LMPGTSSDPN FKRIDVDTQT GKVKGLF
//