Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Formate--tetrahydrofolate ligase

Gene

fhs

Organism
Dehalococcoides mccartyi (strain CBDB1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.UniRule annotation

Pathway:itetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi84 – 918ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciDSP255470:GJXW-557-MONOMER.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate--tetrahydrofolate ligaseUniRule annotation (EC:6.3.4.3UniRule annotation)
Alternative name(s):
Formyltetrahydrofolate synthetaseUniRule annotation
Short name:
FHSUniRule annotation
Short name:
FTHFSUniRule annotation
Gene namesi
Name:fhsUniRule annotation
Ordered Locus Names:cbdbA660
OrganismiDehalococcoides mccartyi (strain CBDB1)
Taxonomic identifieri255470 [NCBI]
Taxonomic lineageiBacteriaChloroflexiDehalococcoidiaDehalococcoidalesDehalococcoidaceaeDehalococcoides

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 597597Formate--tetrahydrofolate ligasePRO_0000199345Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi255470.cbdb_A660.

Structurei

3D structure databases

ProteinModelPortaliQ3ZX40.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the formate--tetrahydrofolate ligase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2759.
KOiK01938.
OMAiCGEIMTM.
OrthoDBiEOG6PCPSP.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3ZX40-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVNDREFHL KELDPVLMKD WEIAEKAEDF LKPVKVLAME LGLTEDEIIP
60 70 80 90 100
HGKYIAKVDF AGVLARLKDR PNGKYIDVTA ITPTPLGEGK STTTMGLVQG
110 120 130 140 150
LGKLGKKVTG AIRQPSSGPT FNIKGSAAGG GLSQCLPLSP FSLGLTGDID
160 170 180 190 200
AVTNSHNLAM VALQARLQHE ANNTDEFLSS RHLTRLDIDP SRVEMKWAMD
210 220 230 240 250
FCAQSLREII MGIGGKTDGY RMPSGFGISV SSEVMAILSV FTSLSDLRER
260 270 280 290 300
MGKIIVAYRK NDEPVTTADL EVDGAMTALL LRAVNPNLLQ TIEGQPVFVH
310 320 330 340 350
AGPFANIAIG QSSIVADRLA LKLADYHVTE SGFGADIGFE KFWNIKCRLS
360 370 380 390 400
GLKPDCAVIV VTARALKMHG GGPKVTPGAP LDPAYTTPNT KLVEKGCQNM
410 420 430 440 450
LAHIQTVKTA GINPVVCINH FAADTHEEID IIRRTAEQAE ARVAVSHHWA
460 470 480 490 500
KGGDGATELA EAVTDACNEP NNFHFLYPEN MPLKKRIETI ARKVYGANGV
510 520 530 540 550
SYTPIAMEKL ARIESMGDTQ FMPTCMVKTH LSLSHDPALK GRPSGFTLPI
560 570 580 590
RDILTYMGAG LVVPVAGDIK LMPGTSSDPN FKRIDVDTQT GKVKGLF
Length:597
Mass (Da):64,493
Last modified:September 27, 2005 - v1
Checksum:i775FDB2876B52CB5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ965256 Genomic DNA. Translation: CAI82827.1.
RefSeqiWP_011309178.1. NC_007356.1.

Genome annotation databases

EnsemblBacteriaiCAI82827; CAI82827; cbdbA660.
KEGGideh:cbdb_A660.
PATRICi21611425. VBIDehSp125902_0557.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ965256 Genomic DNA. Translation: CAI82827.1.
RefSeqiWP_011309178.1. NC_007356.1.

3D structure databases

ProteinModelPortaliQ3ZX40.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi255470.cbdb_A660.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAI82827; CAI82827; cbdbA660.
KEGGideh:cbdb_A660.
PATRICi21611425. VBIDehSp125902_0557.

Phylogenomic databases

eggNOGiCOG2759.
KOiK01938.
OMAiCGEIMTM.
OrthoDBiEOG6PCPSP.

Enzyme and pathway databases

UniPathwayiUPA00193.
BioCyciDSP255470:GJXW-557-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the chlorinated compound-respiring bacterium Dehalococcoides species strain CBDB1."
    Kube M., Beck A., Zinder S.H., Kuhl H., Reinhardt R., Adrian L.
    Nat. Biotechnol. 23:1269-1273(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CBDB1.

Entry informationi

Entry nameiFTHS_DEHMC
AccessioniPrimary (citable) accession number: Q3ZX40
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: September 27, 2005
Last modified: July 22, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.