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Q3ZWQ1 (SYL_DEHSC) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:cbdbA202
OrganismDehalococcoides sp. (strain CBDB1) [Complete proteome] [HAMAP]
Taxonomic identifier255470 [NCBI]
Taxonomic lineageBacteriaChloroflexiDehalococcoidiaDehalococcoidalesDehalococcoidaceaeDehalococcoides

Protein attributes

Sequence length813 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 813813Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000199194

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif580 – 5845"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5831ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q3ZWQ1 [UniParc].

Last modified September 27, 2005. Version 1.
Checksum: C4AB5C597421734B

FASTA81392,974
        10         20         30         40         50         60 
MAEKYNPQET EKKWQDKWAA DRLYHASEDS PKPKWYSLTM FPYTSGNLHI GHWYAEVPAD 

        70         80         90        100        110        120 
CFARYKRLRG FNVMRPVGFD SFGLPAENAA IKHNIHPRIW TLNNVENMRR QLKTIGAMFD 

       130        140        150        160        170        180 
WDREVITCLP EYYKWTQWFF LKLYEAGLAY RAKAPVNWCP SCQAVLANEQ VVDGTCWRCE 

       190        200        210        220        230        240 
TPTTRRDLEQ WFFRITNYAD ELKDHEGLDW PEKITAMQRN WVGKSYGAEV SFALDCPTAF 

       250        260        270        280        290        300 
EKEIKVFTTR PDTIYGVTFM VLAPEHPLVE KITTPENKAA VDAYIKKSRT CTEIERLSTE 

       310        320        330        340        350        360 
REKDGVFTGT YVTNRVNGQK VPVWIGDYVL QSYGTGAVMG VPAHDERDFV FAQKYHLPVI 

       370        380        390        400        410        420 
TVIAPSEYDG KPLEVAYINE GVMLNSGPFN GTPNTEGKEK VCDYLAEHGW GKKTVNYKLR 

       430        440        450        460        470        480 
DWLISRQRYW GAPIPMVYCE KCGIVPVPEK DLPVLLPEDV EFRSGGESPL KYNEGFVNTI 

       490        500        510        520        530        540 
CPVCGGKAKR ETDTMDTFMC SSWYFLRYTS PGYDKGPFDP VKLKYWMPVD LYTGGAEHAV 

       550        560        570        580        590        600 
MHLFYSRFFT KALRDMGIID FGEPFKKLFN QGIIVSNHQK MSKSKGNVVT PDNLVAEVGT 

       610        620        630        640        650        660 
DAVRAYLMFV GPWDQGGEWN DSGLSGMSRW LNRVWNLFTE EYTPQTASAE AERELKRTLH 

       670        680        690        700        710        720 
QTIKKITMDI ERLRFNTVVA ALMELSNSLA KLKETAAISA ENWQNTLQTF ALMLAPVAPH 

       730        740        750        760        770        780 
IAEELWANLG MKYSIHNQNW PTWDEELAKD EVITLIIQVN GKLRERLEMP AGISEAEAKE 

       790        800        810 
TALNSERVKP HLLGKTPVTV IYVPGKLVNI VVK 

« Hide

References

[1]"Genome sequence of the chlorinated compound-respiring bacterium Dehalococcoides species strain CBDB1."
Kube M., Beck A., Zinder S.H., Kuhl H., Reinhardt R., Adrian L.
Nat. Biotechnol. 23:1269-1273(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CBDB1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ965256 Genomic DNA. Translation: CAI82449.1.
RefSeqYP_307365.1. NC_007356.1.

3D structure databases

ProteinModelPortalQ3ZWQ1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING255470.cbdb_A202.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAI82449; CAI82449; cbdbA202.
GeneID3624004.
KEGGdeh:cbdb_A202.
PATRIC21610645. VBIDehSp125902_0171.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycDSP255470:GJXW-174-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYL_DEHSC
AccessionPrimary (citable) accession number: Q3ZWQ1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: September 27, 2005
Last modified: April 16, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries