true2008-02-052023-11-08104TGT_DEHMCGenome sequence of the chlorinated compound-respiring bacterium Dehalococcoides species strain CBDB1.Kube M.Beck A.Zinder S.H.Kuhl H.Reinhardt R.Adrian L.doi:10.1038/nbt11312005Nat. Biotechnol.231269-1273NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]CBDB1Queuine tRNA-ribosyltransferase-likeQ_tRNA_TgtTGTTGT-like_sftRNA_ribo_trans-likeQ_tRNA_tgttgt_generalQUEUINE TRNA-RIBOSYLTRANSFERASEQUEUINE TRNA-RIBOSYLTRANSFERASETGTtRNA-guanine transglycosylaseQueuine tRNA-ribosyltransferase2.4.2.29Guanine insertion enzymetRNA-guanine transglycosylasetgtcbdbA68Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).Binds 1 zinc ion per subunit.Homodimer. Within each dimer, one monomer is responsible for RNA recognition and catalysis, while the other monomer binds to the replacement base PreQ1.Belongs to the queuine tRNA-ribosyltransferase family.Queuine tRNA-ribosyltransferase440571392RNA binding247253RNA binding; important for wobble base 34 recognition271275Proton acceptor93Nucleophile26697147189216304306309335substrate2005-09-2714405794927ed24a6d30a6179be7322f9b29d1MDKSFILNKTSSRSLARRGQLFTAHGKVETPVFCPVGSQAAVKTLTPEDLKSVNINMILSNTYHLYLRPGIPIIKEMGGLHKFMNWDEVILTDSGGYQIFSLANLRKLDEGGVSFRSHIDGSTRYITPEDAVSFQQDLGSDIAMVLDECPHSEASENEVLAAMERTHQWAKRCLAAHTLKIQHLFAIVQGGLSPELRRQSAEYLASLDFPGYALGGLSLGEPKDITFETVRHTLRFLPENKPRYLMGVGAPEDLLEGVSCGVDIFDCVLPTRVARNGAFFSRLGRLNIRNAAFATQKGPIDPECNCYTCRNYSAAYLHHLFRCEEILAYRLATIHNIAFLSNLMQEIRTSIEKDCFEEFKADFLSRYQPTNEAIRIEQKQKWLFGRNGEPPStrue