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Q3ZWC1

- TGT_DEHMC

UniProt

Q3ZWC1 - TGT_DEHMC

Protein

Queuine tRNA-ribosyltransferase

Gene

tgt

Organism
Dehalococcoides mccartyi (strain CBDB1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 1 (27 Sep 2005)
      Previous versions | rss
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    Functioni

    Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).UniRule annotation

    Catalytic activityi

    Guanine(34) in tRNA + queuine = queuosine(34) in tRNA + guanine.UniRule annotation
    Guanine(34) in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine(34) in tRNA + guanine.UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei93 – 931NucleophileUniRule annotation
    Binding sitei94 – 941SubstrateUniRule annotation
    Metal bindingi304 – 3041ZincUniRule annotation
    Metal bindingi306 – 3061ZincUniRule annotation
    Metal bindingi309 – 3091ZincUniRule annotation
    Metal bindingi335 – 3351ZincUniRule annotation

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. queuine tRNA-ribosyltransferase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. queuosine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Queuosine biosynthesis, tRNA processing

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciDSP255470:GJXW-51-MONOMER.
    UniPathwayiUPA00392.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Queuine tRNA-ribosyltransferaseUniRule annotation (EC:2.4.2.29UniRule annotation)
    Alternative name(s):
    Guanine insertion enzymeUniRule annotation
    tRNA-guanine transglycosylaseUniRule annotation
    Gene namesi
    Name:tgtUniRule annotation
    Ordered Locus Names:cbdbA68
    OrganismiDehalococcoides mccartyi (strain CBDB1)
    Taxonomic identifieri255470 [NCBI]
    Taxonomic lineageiBacteriaChloroflexiDehalococcoidiaDehalococcoidalesDehalococcoidaceaeDehalococcoides
    ProteomesiUP000000433: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 392392Queuine tRNA-ribosyltransferasePRO_1000058279Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi255470.cbdb_A68.

    Structurei

    3D structure databases

    ProteinModelPortaliQ3ZWC1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the queuine tRNA-ribosyltransferase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0343.
    KOiK00773.
    OMAiIVQGAFF.
    OrthoDBiEOG6SNDVG.

    Family and domain databases

    Gene3Di3.20.20.105. 1 hit.
    HAMAPiMF_00168. Q_tRNA_Tgt.
    InterProiIPR004803. Queuine_tRNA-ribosylTrfase.
    IPR002616. tRNA_ribo_trans-like.
    [Graphical view]
    PANTHERiPTHR11962. PTHR11962. 1 hit.
    PfamiPF01702. TGT. 1 hit.
    [Graphical view]
    SUPFAMiSSF51713. SSF51713. 1 hit.
    TIGRFAMsiTIGR00430. Q_tRNA_tgt. 1 hit.
    TIGR00449. tgt_general. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q3ZWC1-1 [UniParc]FASTAAdd to Basket

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    MDKSFILNKT SSRSLARRGQ LFTAHGKVET PVFCPVGSQA AVKTLTPEDL    50
    KSVNINMILS NTYHLYLRPG IPIIKEMGGL HKFMNWDEVI LTDSGGYQIF 100
    SLANLRKLDE GGVSFRSHID GSTRYITPED AVSFQQDLGS DIAMVLDECP 150
    HSEASENEVL AAMERTHQWA KRCLAAHTLK IQHLFAIVQG GLSPELRRQS 200
    AEYLASLDFP GYALGGLSLG EPKDITFETV RHTLRFLPEN KPRYLMGVGA 250
    PEDLLEGVSC GVDIFDCVLP TRVARNGAFF SRLGRLNIRN AAFATQKGPI 300
    DPECNCYTCR NYSAAYLHHL FRCEEILAYR LATIHNIAFL SNLMQEIRTS 350
    IEKDCFEEFK ADFLSRYQPT NEAIRIEQKQ KWLFGRNGEP PS 392
    Length:392
    Mass (Da):44,057
    Last modified:September 27, 2005 - v1
    Checksum:i249F2A06D478A54F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ965256 Genomic DNA. Translation: CAI82330.1.
    RefSeqiYP_307246.1. NC_007356.1.

    Genome annotation databases

    EnsemblBacteriaiCAI82330; CAI82330; cbdbA68.
    GeneIDi3622864.
    KEGGideh:cbdb_A68.
    PATRICi21610401. VBIDehSp125902_0051.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ965256 Genomic DNA. Translation: CAI82330.1 .
    RefSeqi YP_307246.1. NC_007356.1.

    3D structure databases

    ProteinModelPortali Q3ZWC1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 255470.cbdb_A68.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAI82330 ; CAI82330 ; cbdbA68 .
    GeneIDi 3622864.
    KEGGi deh:cbdb_A68.
    PATRICi 21610401. VBIDehSp125902_0051.

    Phylogenomic databases

    eggNOGi COG0343.
    KOi K00773.
    OMAi IVQGAFF.
    OrthoDBi EOG6SNDVG.

    Enzyme and pathway databases

    UniPathwayi UPA00392 .
    BioCyci DSP255470:GJXW-51-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.105. 1 hit.
    HAMAPi MF_00168. Q_tRNA_Tgt.
    InterProi IPR004803. Queuine_tRNA-ribosylTrfase.
    IPR002616. tRNA_ribo_trans-like.
    [Graphical view ]
    PANTHERi PTHR11962. PTHR11962. 1 hit.
    Pfami PF01702. TGT. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51713. SSF51713. 1 hit.
    TIGRFAMsi TIGR00430. Q_tRNA_tgt. 1 hit.
    TIGR00449. tgt_general. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the chlorinated compound-respiring bacterium Dehalococcoides species strain CBDB1."
      Kube M., Beck A., Zinder S.H., Kuhl H., Reinhardt R., Adrian L.
      Nat. Biotechnol. 23:1269-1273(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CBDB1.

    Entry informationi

    Entry nameiTGT_DEHMC
    AccessioniPrimary (citable) accession number: Q3ZWC1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: September 27, 2005
    Last modified: October 1, 2014
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3