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Protein

Queuine tRNA-ribosyltransferase

Gene

tgt

Organism
Dehalococcoides mccartyi (strain CBDB1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).UniRule annotation

Catalytic activityi

Guanine(34) in tRNA + queuine = queuosine(34) in tRNA + guanine.UniRule annotation
Guanine(34) in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine(34) in tRNA + guanine.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei93 – 931NucleophileUniRule annotation
Binding sitei94 – 941SubstrateUniRule annotation
Metal bindingi304 – 3041ZincUniRule annotation
Metal bindingi306 – 3061ZincUniRule annotation
Metal bindingi309 – 3091ZincUniRule annotation
Metal bindingi335 – 3351ZincUniRule annotation

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. queuine tRNA-ribosyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. queuosine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Queuosine biosynthesis, tRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciDSP255470:GJXW-51-MONOMER.
UniPathwayiUPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
Queuine tRNA-ribosyltransferaseUniRule annotation (EC:2.4.2.29UniRule annotation)
Alternative name(s):
Guanine insertion enzymeUniRule annotation
tRNA-guanine transglycosylaseUniRule annotation
Gene namesi
Name:tgtUniRule annotation
Ordered Locus Names:cbdbA68
OrganismiDehalococcoides mccartyi (strain CBDB1)
Taxonomic identifieri255470 [NCBI]
Taxonomic lineageiBacteriaChloroflexiDehalococcoidiaDehalococcoidalesDehalococcoidaceaeDehalococcoides
ProteomesiUP000000433 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 392392Queuine tRNA-ribosyltransferasePRO_1000058279Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi255470.cbdb_A68.

Family & Domainsi

Sequence similaritiesi

Belongs to the queuine tRNA-ribosyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0343.
KOiK00773.
OMAiTPRFMPV.
OrthoDBiEOG6SNDVG.

Family and domain databases

Gene3Di3.20.20.105. 1 hit.
HAMAPiMF_00168. Q_tRNA_Tgt.
InterProiIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PANTHERiPTHR11962. PTHR11962. 1 hit.
PfamiPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMiSSF51713. SSF51713. 1 hit.
TIGRFAMsiTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3ZWC1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKSFILNKT SSRSLARRGQ LFTAHGKVET PVFCPVGSQA AVKTLTPEDL
60 70 80 90 100
KSVNINMILS NTYHLYLRPG IPIIKEMGGL HKFMNWDEVI LTDSGGYQIF
110 120 130 140 150
SLANLRKLDE GGVSFRSHID GSTRYITPED AVSFQQDLGS DIAMVLDECP
160 170 180 190 200
HSEASENEVL AAMERTHQWA KRCLAAHTLK IQHLFAIVQG GLSPELRRQS
210 220 230 240 250
AEYLASLDFP GYALGGLSLG EPKDITFETV RHTLRFLPEN KPRYLMGVGA
260 270 280 290 300
PEDLLEGVSC GVDIFDCVLP TRVARNGAFF SRLGRLNIRN AAFATQKGPI
310 320 330 340 350
DPECNCYTCR NYSAAYLHHL FRCEEILAYR LATIHNIAFL SNLMQEIRTS
360 370 380 390
IEKDCFEEFK ADFLSRYQPT NEAIRIEQKQ KWLFGRNGEP PS
Length:392
Mass (Da):44,057
Last modified:September 27, 2005 - v1
Checksum:i249F2A06D478A54F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ965256 Genomic DNA. Translation: CAI82330.1.
RefSeqiWP_011308688.1. NC_007356.1.
YP_307246.1. NC_007356.1.

Genome annotation databases

EnsemblBacteriaiCAI82330; CAI82330; cbdbA68.
KEGGideh:cbdb_A68.
PATRICi21610401. VBIDehSp125902_0051.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ965256 Genomic DNA. Translation: CAI82330.1.
RefSeqiWP_011308688.1. NC_007356.1.
YP_307246.1. NC_007356.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi255470.cbdb_A68.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAI82330; CAI82330; cbdbA68.
KEGGideh:cbdb_A68.
PATRICi21610401. VBIDehSp125902_0051.

Phylogenomic databases

eggNOGiCOG0343.
KOiK00773.
OMAiTPRFMPV.
OrthoDBiEOG6SNDVG.

Enzyme and pathway databases

UniPathwayiUPA00392.
BioCyciDSP255470:GJXW-51-MONOMER.

Family and domain databases

Gene3Di3.20.20.105. 1 hit.
HAMAPiMF_00168. Q_tRNA_Tgt.
InterProiIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PANTHERiPTHR11962. PTHR11962. 1 hit.
PfamiPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMiSSF51713. SSF51713. 1 hit.
TIGRFAMsiTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the chlorinated compound-respiring bacterium Dehalococcoides species strain CBDB1."
    Kube M., Beck A., Zinder S.H., Kuhl H., Reinhardt R., Adrian L.
    Nat. Biotechnol. 23:1269-1273(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CBDB1.

Entry informationi

Entry nameiTGT_DEHMC
AccessioniPrimary (citable) accession number: Q3ZWC1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: September 27, 2005
Last modified: April 29, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.