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Q3ZWC1 (TGT_DEHSC) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Queuine tRNA-ribosyltransferase
EC=2.4.2.29
Alternative name(s):
Guanine insertion enzyme
tRNA-guanine transglycosylase
Gene names
Name:tgt
Ordered Locus Names:cbdbA68
OrganismDehalococcoides sp. (strain CBDB1) [Complete proteome] [HAMAP]
Taxonomic identifier255470 [NCBI]
Taxonomic lineageBacteria › Chloroflexi › Dehalococcoidia › Dehalococcoidales › Dehalococcoidaceae › Dehalococcoides ›

Protein attributes

Sequence length392 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity. HAMAP-Rule MF_00168

Catalytic activity

Guanine34 in tRNA + queuine = queuosine34 in tRNA + guanine. HAMAP-Rule MF_00168

Guanine34 in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine34 in tRNA + guanine. HAMAP-Rule MF_00168

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00168

Pathway

tRNA modification; tRNA-queuosine biosynthesis. HAMAP-Rule MF_00168

Sequence similarities

Belongs to the queuine tRNA-ribosyltransferase family.

Ontologies

Keywords
   Biological processQueuosine biosynthesis
tRNA processing
   LigandMetal-binding
Zinc
   Molecular functionGlycosyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processqueuosine biosynthetic process

Inferred from electronic annotation. Source: HAMAP

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

queuine tRNA-ribosyltransferase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 392392Queuine tRNA-ribosyltransferase HAMAP-Rule MF_00168
PRO_1000058279

Sites

Active site931Nucleophile By similarity
Metal binding3041Zinc By similarity
Metal binding3061Zinc By similarity
Metal binding3091Zinc By similarity
Metal binding3351Zinc By similarity
Binding site941Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q3ZWC1 [UniParc].

Last modified September 27, 2005. Version 1.
Checksum: 249F2A06D478A54F

FASTA39244,057
        10         20         30         40         50         60 
MDKSFILNKT SSRSLARRGQ LFTAHGKVET PVFCPVGSQA AVKTLTPEDL KSVNINMILS 

        70         80         90        100        110        120 
NTYHLYLRPG IPIIKEMGGL HKFMNWDEVI LTDSGGYQIF SLANLRKLDE GGVSFRSHID 

       130        140        150        160        170        180 
GSTRYITPED AVSFQQDLGS DIAMVLDECP HSEASENEVL AAMERTHQWA KRCLAAHTLK 

       190        200        210        220        230        240 
IQHLFAIVQG GLSPELRRQS AEYLASLDFP GYALGGLSLG EPKDITFETV RHTLRFLPEN 

       250        260        270        280        290        300 
KPRYLMGVGA PEDLLEGVSC GVDIFDCVLP TRVARNGAFF SRLGRLNIRN AAFATQKGPI 

       310        320        330        340        350        360 
DPECNCYTCR NYSAAYLHHL FRCEEILAYR LATIHNIAFL SNLMQEIRTS IEKDCFEEFK 

       370        380        390 
ADFLSRYQPT NEAIRIEQKQ KWLFGRNGEP PS

« Hide

References

[1]"Genome sequence of the chlorinated compound-respiring bacterium Dehalococcoides species strain CBDB1."
Kube M., Beck A., Zinder S.H., Kuhl H., Reinhardt R., Adrian L.
Nat. Biotechnol. 23:1269-1273(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CBDB1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AJ965256 Genomic DNA. Translation: CAI82330.1.
RefSeqYP_307246.1. NC_007356.1.

3D structure databases

ProteinModelPortalQ3ZWC1.
ModBaseSearch...

Protein-protein interaction databases

STRING255470.cbdb_A68.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAI82330; CAI82330; cbdbA68.
GeneID3622864.
KEGGdeh:cbdb_A68.
PATRIC21610401. VBIDehSp125902_0051.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0343.
KOK00773.
OMAMSPERSI.
ProtClustDBPRK00112.

Enzyme and pathway databases

BioCycDSP255470:GJXW-51-MONOMER.
UniPathwayUPA00392.

Family and domain databases

Gene3D3.20.20.105. 1 hit.
HAMAPMF_00168. Q_tRNA_Tgt.
InterProIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PANTHERPTHR11962. PTHR11962. 1 hit.
PfamPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMSSF51713. tRNA_ribo_trans. 1 hit.
TIGRFAMsTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTGT_DEHSC
AccessionPrimary (citable) accession number: Q3ZWC1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: September 27, 2005
Last modified: May 29, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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