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Q3ZWC1

- TGT_DEHMC

UniProt

Q3ZWC1 - TGT_DEHMC

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Protein
Queuine tRNA-ribosyltransferase
Gene
tgt, cbdbA68
Organism
Dehalococcoides mccartyi (strain CBDB1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity.UniRule annotation

Catalytic activityi

Guanine(34) in tRNA + queuine = queuosine(34) in tRNA + guanine.UniRule annotation
Guanine(34) in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine(34) in tRNA + guanine.UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei93 – 931Nucleophile By similarity
Binding sitei94 – 941Substrate By similarity
Metal bindingi304 – 3041Zinc By similarity
Metal bindingi306 – 3061Zinc By similarity
Metal bindingi309 – 3091Zinc By similarity
Metal bindingi335 – 3351Zinc By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. queuine tRNA-ribosyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. queuosine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Queuosine biosynthesis, tRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciDSP255470:GJXW-51-MONOMER.
UniPathwayiUPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
Queuine tRNA-ribosyltransferase (EC:2.4.2.29)
Alternative name(s):
Guanine insertion enzyme
tRNA-guanine transglycosylase
Gene namesi
Name:tgt
Ordered Locus Names:cbdbA68
OrganismiDehalococcoides mccartyi (strain CBDB1)
Taxonomic identifieri255470 [NCBI]
Taxonomic lineageiBacteriaChloroflexiDehalococcoidiaDehalococcoidalesDehalococcoidaceaeDehalococcoides
ProteomesiUP000000433: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 392392Queuine tRNA-ribosyltransferaseUniRule annotation
PRO_1000058279Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi255470.cbdb_A68.

Structurei

3D structure databases

ProteinModelPortaliQ3ZWC1.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0343.
KOiK00773.
OMAiIVQGAFF.
OrthoDBiEOG6SNDVG.

Family and domain databases

Gene3Di3.20.20.105. 1 hit.
HAMAPiMF_00168. Q_tRNA_Tgt.
InterProiIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PANTHERiPTHR11962. PTHR11962. 1 hit.
PfamiPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMiSSF51713. SSF51713. 1 hit.
TIGRFAMsiTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3ZWC1-1 [UniParc]FASTAAdd to Basket

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MDKSFILNKT SSRSLARRGQ LFTAHGKVET PVFCPVGSQA AVKTLTPEDL    50
KSVNINMILS NTYHLYLRPG IPIIKEMGGL HKFMNWDEVI LTDSGGYQIF 100
SLANLRKLDE GGVSFRSHID GSTRYITPED AVSFQQDLGS DIAMVLDECP 150
HSEASENEVL AAMERTHQWA KRCLAAHTLK IQHLFAIVQG GLSPELRRQS 200
AEYLASLDFP GYALGGLSLG EPKDITFETV RHTLRFLPEN KPRYLMGVGA 250
PEDLLEGVSC GVDIFDCVLP TRVARNGAFF SRLGRLNIRN AAFATQKGPI 300
DPECNCYTCR NYSAAYLHHL FRCEEILAYR LATIHNIAFL SNLMQEIRTS 350
IEKDCFEEFK ADFLSRYQPT NEAIRIEQKQ KWLFGRNGEP PS 392
Length:392
Mass (Da):44,057
Last modified:September 27, 2005 - v1
Checksum:i249F2A06D478A54F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ965256 Genomic DNA. Translation: CAI82330.1.
RefSeqiYP_307246.1. NC_007356.1.

Genome annotation databases

EnsemblBacteriaiCAI82330; CAI82330; cbdbA68.
GeneIDi3622864.
KEGGideh:cbdb_A68.
PATRICi21610401. VBIDehSp125902_0051.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ965256 Genomic DNA. Translation: CAI82330.1 .
RefSeqi YP_307246.1. NC_007356.1.

3D structure databases

ProteinModelPortali Q3ZWC1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 255470.cbdb_A68.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAI82330 ; CAI82330 ; cbdbA68 .
GeneIDi 3622864.
KEGGi deh:cbdb_A68.
PATRICi 21610401. VBIDehSp125902_0051.

Phylogenomic databases

eggNOGi COG0343.
KOi K00773.
OMAi IVQGAFF.
OrthoDBi EOG6SNDVG.

Enzyme and pathway databases

UniPathwayi UPA00392 .
BioCyci DSP255470:GJXW-51-MONOMER.

Family and domain databases

Gene3Di 3.20.20.105. 1 hit.
HAMAPi MF_00168. Q_tRNA_Tgt.
InterProi IPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view ]
PANTHERi PTHR11962. PTHR11962. 1 hit.
Pfami PF01702. TGT. 1 hit.
[Graphical view ]
SUPFAMi SSF51713. SSF51713. 1 hit.
TIGRFAMsi TIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the chlorinated compound-respiring bacterium Dehalococcoides species strain CBDB1."
    Kube M., Beck A., Zinder S.H., Kuhl H., Reinhardt R., Adrian L.
    Nat. Biotechnol. 23:1269-1273(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CBDB1.

Entry informationi

Entry nameiTGT_DEHMC
AccessioniPrimary (citable) accession number: Q3ZWC1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: September 27, 2005
Last modified: May 14, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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