ID   NS1A_HASV4              Reviewed;         920 AA.
AC   Q3ZN07;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   27-SEP-2005, sequence version 1.
DT   05-MAY-2009, entry version 27.
DE   RecName: Full=Non-structural polyprotein 1A;
DE   Contains:
DE     RecName: Full=Protein p19;
DE   Contains:
DE     RecName: Full=Transmembrane protein 1A;
DE   Contains:
DE     RecName: Full=Serine protease p27;
DE              Short=p27;
DE              EC=3.4.21.-;
DE   Contains:
DE     RecName: Full=Protein p20';
GN   Name=ORF1;
OS   Human astrovirus-4 (HAstV-4).
OC   Viruses; ssRNA positive-strand viruses, no DNA stage; Astroviridae;
OC   Mamastrovirus.
OX   NCBI_TaxID=35300;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Dresden;
RA   Barthel J., Rethwilm A., Rohayem J.;
RT   "Molecular characterization of human astrovirus type 4.";
RL   Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Non-structural polyprotein 1A encodes for protease and
CC       some functions involved in viral replication/transcription.
CC   -!- CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate
CC       + RNA(n+1).
CC   -!- SUBCELLULAR LOCATION: Host membrane; Multi-pass membrane protein
CC       (Potential).
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Ribosomal frameshifting; Named isoforms=2;
CC       Name=nsp1a;
CC         IsoId=Q3ZN07-1; Sequence=Displayed;
CC       Name=nsp1ab;
CC         IsoId=Q3ZN06-1; Sequence=External;
CC         Note=Generated by a ribosomal frameshift at position 900;
CC   -!- PTM: Cleaved presumably by viral and host proteases.
CC   -!- SIMILARITY: Belongs to the astroviridae polyprotein 1A family.
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DR   EMBL; AY720891; AAW51877.1; -; Genomic_RNA.
DR   MEROPS; S01.109; -.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0008234; F:cysteine-type peptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006410; P:transcription, RNA-dependent; IEA:UniProtKB-KW.
PE   3: Inferred from homology;
KW   Coiled coil; Hydrolase; Membrane; Protease; Ribosomal frameshifting;
KW   RNA replication; Thiol protease; Transmembrane.
FT   CHAIN         1    920       Non-structural polyprotein 1A.
FT                                /FTId=PRO_0000327330.
FT   CHAIN         1    175       Protein p19 (Potential).
FT                                /FTId=PRO_0000327331.
FT   CHAIN       176    419       Transmembrane protein 1A (Potential).
FT                                /FTId=PRO_0000327332.
FT   CHAIN       420    664       Serine protease p27 (Potential).
FT                                /FTId=PRO_0000327333.
FT   CHAIN       665    920       Protein p20' (Potential).
FT                                /FTId=PRO_0000327334.
FT   TRANSMEM    239    259       Potential.
FT   TRANSMEM    286    306       Potential.
FT   TRANSMEM    313    333       Potential.
FT   TRANSMEM    344    364       Potential.
FT   ACT_SITE    461    461       Charge relay system; for serine protease
FT                                activity (By similarity).
FT   ACT_SITE    489    489       Charge relay system; for serine protease
FT                                activity (By similarity).
FT   ACT_SITE    551    551       Charge relay system; for serine protease
FT                                activity (By similarity).
FT   SITE        175    176       Cleavage (Potential).
FT   SITE        419    420       Cleavage (Potential).
FT   SITE        664    665       Cleavage (Potential).
SQ   SEQUENCE   920 AA;  103386 MW;  52836952DD2DCD85 CRC64;
     MAHGEPYYSS KPDKDFNFGS TMARRQMTPT MVAKLPNFVR NSPQAYDWIV RGLIFPTTGK
     TYFQRVVVIT GGLEDGTYGS FVFDGREWVE IYPIEHLNLM SSLKLIHKAN ALQERLRLSQ
     EEKATLALDV QFLQHENVRL KELIPKPEPR KIQMKWIIVG AVLTFLSLIP GGYAQSQINN
     TIFTDMIAAC KYSTETLTEN LDLRIKLALA NITISDKLDA VRQILNFAFV PRAHWLRTVF
     YYIHYYEMWN IFMFVLAIGT VMRSARPGTD LITLATSHLS GFRMAVLPTI PFHTTMTLWV
     MNTLMVCYYF DNLLAITLAI LAPILGIIFL CFMEDSNYVS QIRGLIATAV LIAGGHACLT
     LTGTTTSLFV VILTCRFIRM ATVFIGTRFE IRDANGKVVA TVPTRIKNVA FDFFQKLKQS
     GVRVGVNEFV VIKPGALCVI DTPEGKGTGF FSGNDIVTAA HAVGNNTFVN VCYEGLMYEA
     KVRYMPEKDI AFITCPGDLH PTARLKLSKN PDYSCVTVMA YVNEDLVVST AAAMVHGNTL
     SYAVRTQDGM SGAPVCDKYG RVLAVHQTNT GYTGGAVIID PTDFHPVKAP SRVELLKEEI
     ERLKAQLNSA AENPATAVTQ QPVVTLEQKS VSDSDVVDLV RTAMEREMKV LRDEINGILA
     PFLQKKKGKT KHGRGRVRRN LRKGVKLLTE EEYRELLEKG LDRETFLDLI DRIIGERSGY
     PDYDDEDYYD EDDDGWGVVG DDVEFDYTEV INFDQAKPTP APRTVKPKTC PEPEAETQPL
     DLSQKKEKQL EHEQQVVKST KPQKNEPQPY SQTYGKAPIW ESYDFDWDED DAKFILPAPH
     RLTKADEIVL GSKIVKLRTI IETAIKTQNY SALPEAVFEL DKAAYEAGLE GFLQRVKSKN
     KAPKNYKGPQ KTKGPKTITH
//