Q3ZN07 (NS1A_HASV4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-structural polyprotein 1A

Cleaved into the following 4 chains:

1. Protein p19
2. Transmembrane protein 1A
3. Serine protease p27
   Short name=p27
   EC=3.4.21.-
4. Protein p20'

Gene names

Name:

ORF1

Organism

Human astrovirus-4 (HAstV-4) [Complete proteome]

Taxonomic identifier

35300 [NCBI]

Taxonomic lineage

Viruses › ssRNA positive-strand viruses, no DNA stage › Astroviridae › Mamastrovirus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	920 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Non-structural polyprotein 1A encodes for protease and some functions involved in viral replication/transcription.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subcellular location	Host membrane; Multi-pass membrane protein Potential.
Post-translational modification	Cleaved presumably by viral and host proteases.
Sequence similarities	Belongs to the astroviridae polyprotein 1A family.

Ontologies

Keywords
Biological process	RNA replication
Cellular component	Host membrane Membrane
Coding sequence diversity	Ribosomal frameshifting
Domain	Coiled coil Transmembrane Transmembrane helix
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	host cell membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro serine-type exopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by ribosomal frameshifting. [Align] [Select]

Isoform nsp1a (identifier: Q3ZN07-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform nsp1ab (identifier: Q3ZN06-1)

The sequence of this isoform can be found in the external entry Q3ZN06.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Generated by a ribosomal frameshift at position 900.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 920	920	Non-structural polyprotein 1A		PRO_0000327330
	Chain	1 – 175	175	Protein p19 Potential		PRO_0000327331
	Chain	176 – 419	244	Transmembrane protein 1A Potential		PRO_0000327332
	Chain	420 – 664	245	Serine protease p27 Potential		PRO_0000327333
	Chain	665 – 920	256	Protein p20' Potential		PRO_0000327334
Regions
	Transmembrane	239 – 259	21	Helical; Potential
	Transmembrane	286 – 306	21	Helical; Potential
	Transmembrane	313 – 333	21	Helical; Potential
	Transmembrane	344 – 364	21	Helical; Potential
Sites
	Active site	461	1	Charge relay system; for serine protease activity By similarity
	Active site	489	1	Charge relay system; for serine protease activity By similarity
	Active site	551	1	Charge relay system; for serine protease activity By similarity
	Site	175 – 176	2	Cleavage Potential
	Site	419 – 420	2	Cleavage Potential
	Site	664 – 665	2	Cleavage Potential

Sequences

	Sequence		Length	Mass (Da)
	Isoform nsp1a [UniParc]. Last modified September 27, 2005. Version 1. Checksum: 52836952DD2DCD85	FASTA	920	103,386
	10 20 30 40 50 60 MAHGEPYYSS KPDKDFNFGS TMARRQMTPT MVAKLPNFVR NSPQAYDWIV RGLIFPTTGK 70 80 90 100 110 120 TYFQRVVVIT GGLEDGTYGS FVFDGREWVE IYPIEHLNLM SSLKLIHKAN ALQERLRLSQ 130 140 150 160 170 180 EEKATLALDV QFLQHENVRL KELIPKPEPR KIQMKWIIVG AVLTFLSLIP GGYAQSQINN 190 200 210 220 230 240 TIFTDMIAAC KYSTETLTEN LDLRIKLALA NITISDKLDA VRQILNFAFV PRAHWLRTVF 250 260 270 280 290 300 YYIHYYEMWN IFMFVLAIGT VMRSARPGTD LITLATSHLS GFRMAVLPTI PFHTTMTLWV 310 320 330 340 350 360 MNTLMVCYYF DNLLAITLAI LAPILGIIFL CFMEDSNYVS QIRGLIATAV LIAGGHACLT 370 380 390 400 410 420 LTGTTTSLFV VILTCRFIRM ATVFIGTRFE IRDANGKVVA TVPTRIKNVA FDFFQKLKQS 430 440 450 460 470 480 GVRVGVNEFV VIKPGALCVI DTPEGKGTGF FSGNDIVTAA HAVGNNTFVN VCYEGLMYEA 490 500 510 520 530 540 KVRYMPEKDI AFITCPGDLH PTARLKLSKN PDYSCVTVMA YVNEDLVVST AAAMVHGNTL 550 560 570 580 590 600 SYAVRTQDGM SGAPVCDKYG RVLAVHQTNT GYTGGAVIID PTDFHPVKAP SRVELLKEEI 610 620 630 640 650 660 ERLKAQLNSA AENPATAVTQ QPVVTLEQKS VSDSDVVDLV RTAMEREMKV LRDEINGILA 670 680 690 700 710 720 PFLQKKKGKT KHGRGRVRRN LRKGVKLLTE EEYRELLEKG LDRETFLDLI DRIIGERSGY 730 740 750 760 770 780 PDYDDEDYYD EDDDGWGVVG DDVEFDYTEV INFDQAKPTP APRTVKPKTC PEPEAETQPL 790 800 810 820 830 840 DLSQKKEKQL EHEQQVVKST KPQKNEPQPY SQTYGKAPIW ESYDFDWDED DAKFILPAPH 850 860 870 880 890 900 RLTKADEIVL GSKIVKLRTI IETAIKTQNY SALPEAVFEL DKAAYEAGLE GFLQRVKSKN 910 920 KAPKNYKGPQ KTKGPKTITH
	Isoform nsp1ab [UniParc]. See Q3ZN06.		–	–

References

[1]	"Molecular characterization of human astrovirus type 4." Barthel J., Rethwilm A., Rohayem J. Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Dresden.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY720891 Genomic RNA. Translation: AAW51877.1.
3D structure databases
ProteinModelPortal	Q3ZN07.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR022068. Astroviridae_polyprotein_1. IPR009003. Pept_cys/ser_Trypsin-like. IPR001730. Peptidase_C4. [Graphical view]
Pfam	PF12285. DUF3621. 1 hit. PF00863. Peptidase_C4. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NS1A_HASV4

Accession

Primary (citable) accession number: Q3ZN07

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	September 27, 2005
Last modified:	November 16, 2011
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program