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Q3ZK57

- VP3_ROT41

UniProt

Q3ZK57 - VP3_ROT41

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Protein

Protein VP3

Gene
N/A
Organism
Rotavirus A (isolate Human/Belgium/B4106/2000 G3-P11[14]-I2-R2-C2-M3-A9-N2-T6-E5-H3) (RV-A) (Rotavirus A (isolate B4106))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Multifunctional enzyme involved in mRNA capping. Catalyzes the formation of the 5' cap structure on the viral plus-strand transcripts. Specifically binds to GTP and displays guanylyltransferase and methyltransferase activities. Has affinity for ssRNA but not for dsRNA. Capping activity is non-specific and caps RNAs that initiate with either a G or an A residue. Together with VP1 polymerase, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are capped by VP3 and extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. DLPs probably have an RNA triphosphatase activity as well, whereas open cores don't (By similarity).By similarity

Catalytic activityi

GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.
S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. mRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
  3. mRNA guanylyltransferase activity Source: UniProtKB-EC
  4. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. viral process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

mRNA capping, mRNA processing

Keywords - Ligandi

GTP-binding, Nucleotide-binding, RNA-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Protein VP3
Including the following 2 domains:
mRNA guanylyltransferase (EC:2.7.7.50)
mRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.56)
OrganismiRotavirus A (isolate Human/Belgium/B4106/2000 G3-P11[14]-I2-R2-C2-M3-A9-N2-T6-E5-H3) (RV-A) (Rotavirus A (isolate B4106))
Taxonomic identifieri578843 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000008655: Genome

Subcellular locationi

Virion Curated
Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging (Potential).Curated

GO - Cellular componenti

  1. viral nucleocapsid Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 835835Protein VP3PRO_0000368067Add
BLAST

Interactioni

Subunit structurei

Interacts with VP1 (Potential). Interacts with VP2 (By similarity).By similarityCurated

Family & Domainsi

Sequence similaritiesi

Belongs to the rotavirus VP3 family.Curated

Family and domain databases

InterProiIPR011181. VP3_Rotav.
[Graphical view]
PfamiPF06929. Rotavirus_VP3. 1 hit.
[Graphical view]
PIRSFiPIRSF004015. LigT_rotavirus. 1 hit.
PROSITEiPS51589. SAM_MT56_VP3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3ZK57 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKVLALRHSV AQVYADTQVY THDDTKDSYE NAFLISNLTT HNILYFNYSV
60 70 80 90 100
KTLEILNKSG IAAIEIQSLE ELFTLIRCNF TYDYENNVVY LHDYSYYTNN
110 120 130 140 150
EIRTDQHWIT KTNIEEYLLP GWKLTYVGYN GNDTRGHYNF SFTCQNAATD
160 170 180 190 200
DDIIIEYIYS EALDFQNFML KKIKERMTTS LPIARLSNRV FRDKLFPLLV
210 220 230 240 250
KKHKRVINVG PRNESMFTFL NFPSIRQFSN GPYLVKNTIK LKQERWLGKR
260 270 280 290 300
VSQFDIGQYK NMMNVITTIY HYYNLYQEKP IIYMVGSAPS YWIYDVRQYS
310 320 330 340 350
EFLFETWDPL DTPYSSIHHK ELFFEKDIGK LKDNSILYID IRTDRGNVDW
360 370 380 390 400
KEWRKIVELQ TINNLDLAYK YLATGKSKVC CVKLTAMDLE LPVSAKLLHH
410 420 430 440 450
PTTEIRSEFY LLLDIWDVNN VKRFIPKGVL YSFINNVITD NVFIQSPFKI
460 470 480 490 500
RTSMSDYIVA LYALSNDFNN RADIINLINN QKQSLITVRI NNTFKDEPKV
510 520 530 540 550
GFKNIYDWTF LPTDFDTTNA IVTSYDGCLG MFGLSISLAS KPTGNNHLFI
560 570 580 590 600
LNGTDKYYKL DQFANHTGIS RRSHQIRFSE SATSYSGYIF RDLSNNNFNL
610 620 630 640 650
IGTNVENSVS GHVYNALIYY RYNYSFDLKR WIYLHSIEKV DIEGGKYYEH
660 670 680 690 700
APIELIYACR SAKEFALLQD DLTVLRYANE IENYINKVYS ITYADDPNYF
710 720 730 740 750
IGIKFDNIPY TYDVKVPHLT FGVLYISDNM IPDVVKIMKS MKQELFGMDV
760 770 780 790 800
TTSYTYMLSD GIYVANVSGV LATYFKMYNL FYKNQITFGQ SRMFIPHITL
810 820 830
SFRNNKTVRI ETTKLRIKSI YLRKIRGDTM FDMSE
Length:835
Mass (Da):97,978
Last modified:September 27, 2005 - v1
Checksum:iF4DFDE18A2D06781
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY740739 Genomic RNA. Translation: AAU43797.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY740739 Genomic RNA. Translation: AAU43797.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR011181. VP3_Rotav.
[Graphical view ]
Pfami PF06929. Rotavirus_VP3. 1 hit.
[Graphical view ]
PIRSFi PIRSF004015. LigT_rotavirus. 1 hit.
PROSITEi PS51589. SAM_MT56_VP3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Full genomic analysis of human rotavirus strain B4106 and lapine rotavirus strain 30/96 provides evidence for interspecies transmission."
    Matthijnssens J., Rahman M., Martella V., Xuelei Y., De Vos S., De Leener K., Ciarlet M., Buonavoglia C., Van Ranst M.
    J. Virol. 80:3801-3810(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiVP3_ROT41
AccessioniPrimary (citable) accession number: Q3ZK57
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: September 27, 2005
Last modified: October 1, 2014
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3