Q3ZCN5 (OTOGL_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Otogelin-like protein

Gene names

Name:	OTOGL
Synonyms:	C12orf64

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	2332 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Subcellular location	Secreted Potential.
Tissue specificity	Expressed at high levels in fetal inner ear and heart. Low levels in fetal skeletal muscle, kidney, spleen and colon. Not detected in fetal liver, lung, brain, nor in fetal stomach. In adult tissues, highest levels in brain, kidney, heart and retina. Relatively low levels in lung, spleen and duodenum. Not detected in adult skeletal muscle, liver, nor testis. Ref.4
Involvement in disease	Deafness, autosomal recessive, 84B (DFNB84B) [MIM:614944]: A form of non-syndromic deafness characterized by congenital, non-progressive, sensorineural, symmetric hearing loss. Vestibular hypofunction is rarely observed. Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.4
Sequence similarities	Belongs to the otogelin family. Contains 1 CTCK (C-terminal cystine knot-like) domain. Contains 2 TIL (trypsin inhibitory-like) domains. Contains 4 VWFD domains.
Sequence caution	The sequence BAC04877.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAC11376.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Disease	Deafness Non-syndromic deafness
Domain	Repeat Signal
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	L-arabinose metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	alpha-N-arabinofuranosidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 2332	2310	Otogelin-like protein		PRO_0000316765
Regions
	Domain	113 – 326	214	VWFD 1
	Domain	381 – 434	54	TIL 1
	Domain	473 – 683	211	VWFD 2
	Domain	736 – 791	56	TIL 2
	Domain	938 – 1141	204	VWFD 3
	Domain	1514 – 1734	221	VWFD 4
	Domain	2240 – 2332	93	CTCK
	Compositional bias	1908 – 2095	188	Cys-rich
Amino acid modifications
	Glycosylation	135	1	N-linked (GlcNAc...) Potential
	Glycosylation	425	1	N-linked (GlcNAc...) Potential
	Glycosylation	464	1	N-linked (GlcNAc...) Potential
	Glycosylation	817	1	N-linked (GlcNAc...) Potential
	Glycosylation	867	1	N-linked (GlcNAc...) Potential
	Glycosylation	1280	1	N-linked (GlcNAc...) Potential
	Glycosylation	1583	1	N-linked (GlcNAc...) Potential
	Glycosylation	2177	1	N-linked (GlcNAc...) Potential
	Disulfide bond	136 ↔ 144		By similarity
	Disulfide bond	2240 ↔ 2296		By similarity
	Disulfide bond	2261 ↔ 2310		By similarity
	Disulfide bond	2272 ↔ 2327		By similarity
	Disulfide bond	2276 ↔ 2329		By similarity
Natural variations
	Natural variant	1378	1	C → R. Ref.4		VAR_069040
	Natural variant	2276	1	C → F in a breast cancer sample; somatic mutation. Ref.5		VAR_038388
Experimental info
	Sequence conflict	2062	1	I → V in BAC04877. Ref.2
	Sequence conflict	2062	1	I → V in AAI01018. Ref.3
	Sequence conflict	2062	1	I → V in AAI01019. Ref.3
	Sequence conflict	2120	1	A → T in BAC04877. Ref.2
	Sequence conflict	2120	1	A → T in BAC11376. Ref.2
	Sequence conflict	2120	1	A → T in AAI01018. Ref.3
	Sequence conflict	2120	1	A → T in AAI01019. Ref.3
	Sequence conflict	2225	1	N → S in BAC11376. Ref.2
	Sequence conflict	2225	1	N → S in AAI01018. Ref.3
	Sequence conflict	2225	1	N → S in AAI01019. Ref.3
	Sequence conflict	2253	1	K → E in BAC04877. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q3ZCN5 [UniParc]. Last modified February 6, 2013. Version 5. Checksum: D43BCCDB7727CB80	FASTA	2,332	262,091
	10 20 30 40 50 60 MIPWSIFLLH VLLFSLQEYI CASSILMGTS KNGFNENRQK RALLAAQFEA TSPRYFFHDA 70 80 90 100 110 120 INWGESKIKG SCPYECLNGA FCSKTGTCDC QIFQALGTRC QIIPNMGNGR DGICKTWGQY 130 140 150 160 170 180 HFETFDGIYY YFPGNCSYIF AKDCGDLEPR YTVWVHNSPK CLGSVYSCYR SISLFFSNQE 190 200 210 220 230 240 EIRIYGHEIK KNGISLTLPQ TIGQIFIEKL ADYILVKTTF GFSLAWDGIS GIYLKLSEDH 250 260 270 280 290 300 KGKSCGLCGN YNDIQSDDFI ILQEDYTEDI AMFANSWSVQ TPDDTKCVLT PSDFPNPCSS 310 320 330 340 350 360 GMPAFEAIFF KCQILLQFPF LSCHEYIDPY LYIASCVNDL CKTDDDETYC RAATEYARAC 370 380 390 400 410 420 SHAGYPIQDW RDDFPACTDK CDDSFVHRDC ISCCPPTCTF EKQCLGSNLH CLDGCYCPDG 430 440 450 460 470 480 LVMDNGTCIS LENCPCGFHG LAYSVGSKIE QECTECVCVG GVWNCTEQDC PVQCSVVGDS 490 500 510 520 530 540 HFTTFDGRHY SFIGMCQYIL VKGTGKDKFT ITLQKAPCEQ NLGLVCLQSI TLILEDDFNK 550 560 570 580 590 600 QVTLGRGGQI LTSPNQGFNL NGIVEIQTLS SLFILLKTTF GLKILFAIDG ERIYIQLTSA 610 620 630 640 650 660 WKRRTLGLCG TFNGNIRDDF LSPSGMIEGT PQLHANAWRV SSTCFAPVHV PVVDPCNINQ 670 680 690 700 710 720 QNIGYAAHCD VIHQELFAPC HIYISPGLYY QLCRHDACKC GSSCLCNALA HYAYLCGQHG 730 740 750 760 770 780 VPIDFRTQIS FCAVVCQKGM LYHHCSSFCL HSCISLSSPE QCSDDCAEGC NCPEGKFYED 790 800 810 820 830 840 TLNFCVPIFH CRCHYRGSVY QPGELIPTPS GLCQCSNGTV KCDELATPSA VHICPEGKEY 850 860 870 880 890 900 FDCRFPDPEL PAGGVNCETT CANLAMNFTC TPSSPCISGC VCAPGMAEHR GKCYVPESCP 910 920 930 940 950 960 CIWKDWEYLS GEVIATPCYT CVCRRGMFNC TYYPCPAVCT IYGDRHYYSF DGLEYDYISD 970 980 990 1000 1010 1020 CQVFLIKSAD DSDISVIAQN KKCFDNDIVC SKSVLISVGD TEIYLNDTPY KQKQSGFFLE 1030 1040 1050 1060 1070 1080 NKSTYQLWKA GYYIVVYFPE KDITILWDRK TTIHIKVGPQ WKNKLSGLCG NFDKCTSNDM 1090 1100 1110 1120 1130 1140 TTSNNLEVRN ARVFGDSWAL GQCESPDETI KPCEAHQNKF PYAKKECSIL YSDIFASCRN 1150 1160 1170 1180 1190 1200 VIDVTSFAKN CHEDTCNCNL GGDCECLCTS IAAYAYKCCQ EGISIHWRSS TVCSLDCEYY 1210 1220 1230 1240 1250 1260 NEGLGEGPYM LASYGQSGLV LGANMTSRSV FCLPRSSVHT SLFFYFMITP GLFKEKVSSL 1270 1280 1290 1300 1310 1320 ALVSLESAER PNYFLYVHDN DTLSLELWEA NSAFHRRATF FHHQGLWIPG YSAFELYSKK 1330 1340 1350 1360 1370 1380 GFFIIFTDSS VKASKYDDSE EFKHSSSFSI EEIQAAVPYR KMCEWRYEPC ATPCFKTCSD 1390 1400 1410 1420 1430 1440 PEALACKFLP PVEGCLPYCP KNMILDEVTL KCVYPRDCIP VIPTEPTLMP PAKPTVPITV 1450 1460 1470 1480 1490 1500 FDMLTPTTGL ECEPQKFDPV YDCSQYICLN MEWQLYNWSL NCPKDVEMPD CGFRGRPVQV 1510 1520 1530 1540 1550 1560 NSDICCPEWE CPCRCSMLSE LSIITFDGNN AALYSMASYI LVRIPGEIIV AHIEKCSMNQ 1570 1580 1590 1600 1610 1620 NGNSLKKLAP SGRISGLCFK KLNVTTPIHK IIVNRLARKV EVDSIVVPLP FSSQELSIED 1630 1640 1650 1660 1670 1680 SGSMYVITTP AGLIIKWSHL TGIIDIHFGF RFNLSSYTEG LCGICNEDPD DDLRMQNGTI 1690 1700 1710 1720 1730 1740 ITNMEDIGLF IESWEIEKSF EVTMRRPVRN CTEHDCSQCI DLLNRRIFIP CHDKVSPEDF 1750 1760 1770 1780 1790 1800 CEKMWINYTY FWNYECDALS AYVALCNKFD ICIQWRTPDY CSLSCPEGKE YQPCVRPCEA 1810 1820 1830 1840 1850 1860 RTCLNQWFYG HTSCLNLRED CVCKVGTILH RPHSAQCIPE KECACTDSED QPRTAGEIWN 1870 1880 1890 1900 1910 1920 GGIDECTLYK CLENGSIIPI EPDCDEEPTP VCEREAEVVM GIIDKWTCCS KEVCGCDTTL 1930 1940 1950 1960 1970 1980 CETSIPTCTN SQKLIVGHSP LSCCPQYKCE CDPLKCPSIS TPECREDQFM IQVRQEEPCC 1990 2000 2010 2020 2030 2040 FSPFCVCESC TKPVPLCHDG EFLTVDLNST HFCCPQYYCV CEPNLCPMPL LNCAEDMNLV 2050 2060 2070 2080 2090 2100 KENVSGQCCP TWHCECNCEN LIMPTCEVGE FTAIDHNFQS DCGCIQYLCE KDDVCVFQEV 2110 2120 2130 2140 2150 2160 SVLNPGQSMI KYLEEDFCYA IECLEEKDNH TGFHTLNFTL VNCSKKCDVH QVYTPSPSDY 2170 2180 2190 2200 2210 2220 GCCGTCKNVS CKFHMENGTS VVYAVGSTWH YNCTTYECVK TDEGAIILNY TMVCPPFNET 2230 2240 2250 2260 2270 2280 ECKMNEGIVK LYNEGCCKIC KREERICQKV IIKSVIRKQD CMSQSPINVA SCDGKCPSAT 2290 2300 2310 2320 2330 IYNINIESHL RFCKCCRENG VRNLSVPLYC SGNGTEIMYT LQEPIDCTCQ WN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The finished DNA sequence of human chromosome 12." Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R. , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A. Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1915-2332. Tissue: Placenta and Vascular endothelial cell.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1938-2332.
[4]	"Mutations in OTOGL, encoding the inner ear protein otogelin-like, cause moderate sensorineural hearing loss." Yariz K.O., Duman D., Seco C.Z., Dallman J., Huang M., Peters T.A., Sirmaci A., Lu N., Schraders M., Skromne I., Oostrik J., Diaz-Horta O., Young J.I., Tokgoz-Yilmaz S., Konukseven O., Shahin H., Hetterschijt L., Kanaan M. , Oonk A.M., Edwards Y.J., Li H., Atalay S., Blanton S., Desmidt A.A., Liu X.Z., Pennings R.J., Lu Z., Chen Z.Y., Kremer H., Tekin M. Am. J. Hum. Genet. 91:872-882(2012) [PubMed] [Europe PMC] [Abstract] Cited for: INVOLVEMENT IN DFNB84B, VARIANT ARG-1378, TISSUE SPECIFICITY.
[5]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] PHE-2276.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC078817 Genomic DNA. No translation available. AC083812 Genomic DNA. No translation available. AC092945 Genomic DNA. No translation available. AK075060 mRNA. Translation: BAC11376.1. Different initiation. AK096852 mRNA. Translation: BAC04877.1. Different initiation. BC101017 mRNA. Translation: AAI01018.3. BC101018 mRNA. Translation: AAI01019.2.
IPI	IPI01021306.
UniGene	Hs.723594.
3D structure databases
ProteinModelPortal	Q3ZCN5.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000298820.
PTM databases
PhosphoSite	Q3ZCN5.
Polymorphism databases
DMDM	218512154.
Proteomic databases
PaxDb	Q3ZCN5.
PRIDE	Q3ZCN5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000547103; ENSP00000447211; ENSG00000165899.
UCSC	uc001szd.3. human. uc021rba.1. human.
Organism-specific databases
GeneCards	GC12P080603.
H-InvDB	HIX0018423.
HGNC	HGNC:26901. OTOGL.
HPA	HPA040364.
MIM	614925. gene. 614944. phenotype.
neXtProt	NX_Q3ZCN5.
Orphanet	90636. Autosomal recessive nonsyndromic sensorineural deafness type DFNB.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG325585.
HOGENOM	HOG000115301.
HOVERGEN	HBG107710.
InParanoid	Q3ZCN5.
Gene expression databases
ArrayExpress	Q3ZCN5.
Bgee	Q3ZCN5.
CleanEx	HS_C12orf64.
Genevestigator	Q3ZCN5.
Family and domain databases
InterPro	IPR007934. AbfB. IPR006207. Cys_knot_C. IPR002919. TIL_dom. IPR014853. Unchr_dom_Cys-rich. IPR001846. VWF_type-D. [Graphical view]
Pfam	PF05270. AbfB. 1 hit. PF08742. C8. 4 hits. PF01826. TIL. 2 hits. PF00094. VWD. 4 hits. [Graphical view]
SMART	SM00832. C8. 4 hits. SM00041. CT. 1 hit. SM00216. VWD. 4 hits. [Graphical view]
SUPFAM	SSF110221. AbfB. 1 hit. SSF57567. Cysrich_TIL. 5 hits.
PROSITE	PS01225. CTCK_2. 1 hit. PS51233. VWFD. 4 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	35512394.
SOURCE	Search...

Entry information

Entry name

OTOGL_HUMAN

Accession

Primary (citable) accession number: Q3ZCN5
Secondary accession number(s): F8W0C3 , Q495U8, Q8N8G5, Q8NC28

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	February 6, 2013
Last modified:	May 29, 2013
This is version 63 of the entry and version 5 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.