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Protein

Proteasome subunit alpha type-4

Gene

PSMA4

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).By similarity

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Threonine protease

Enzyme and pathway databases

ReactomeiR-BTA-1169091 Activation of NF-kappaB in B cells
R-BTA-1234176 Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha
R-BTA-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-BTA-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-BTA-174113 SCF-beta-TrCP mediated degradation of Emi1
R-BTA-174154 APC/C:Cdc20 mediated degradation of Securin
R-BTA-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-BTA-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-BTA-187577 SCF(Skp2)-mediated degradation of p27/p21
R-BTA-195253 Degradation of beta-catenin by the destruction complex
R-BTA-202424 Downstream TCR signaling
R-BTA-2467813 Separation of Sister Chromatids
R-BTA-2871837 FCERI mediated NF-kB activation
R-BTA-349425 Autodegradation of the E3 ubiquitin ligase COP1
R-BTA-350562 Regulation of ornithine decarboxylase (ODC)
R-BTA-382556 ABC-family proteins mediated transport
R-BTA-450408 AUF1 (hnRNP D0) binds and destabilizes mRNA
R-BTA-4608870 Asymmetric localization of PCP proteins
R-BTA-4641257 Degradation of AXIN
R-BTA-4641258 Degradation of DVL
R-BTA-5358346 Hedgehog ligand biogenesis
R-BTA-5607761 Dectin-1 mediated noncanonical NF-kB signaling
R-BTA-5607764 CLEC7A (Dectin-1) signaling
R-BTA-5610780 Degradation of GLI1 by the proteasome
R-BTA-5610785 GLI3 is processed to GLI3R by the proteasome
R-BTA-5632684 Hedgehog 'on' state
R-BTA-5658442 Regulation of RAS by GAPs
R-BTA-5668541 TNFR2 non-canonical NF-kB pathway
R-BTA-5676590 NIK-->noncanonical NF-kB signaling
R-BTA-5687128 MAPK6/MAPK4 signaling
R-BTA-5689603 UCH proteinases
R-BTA-5689880 Ub-specific processing proteases
R-BTA-68827 CDT1 association with the CDC6:ORC:origin complex
R-BTA-68949 Orc1 removal from chromatin
R-BTA-69017 CDK-mediated phosphorylation and removal of Cdc6
R-BTA-69229 Ubiquitin-dependent degradation of Cyclin D1
R-BTA-69481 G2/M Checkpoints
R-BTA-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
R-BTA-8852276 The role of GTSE1 in G2/M progression after G2 checkpoint
R-BTA-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-BTA-8939236 RUNX1 regulates transcription of genes involved in differentiation of HSCs
R-BTA-8939902 Regulation of RUNX2 expression and activity
R-BTA-8941858 Regulation of RUNX3 expression and activity
R-BTA-8948751 Regulation of PTEN stability and activity
R-BTA-9020702 Interleukin-1 signaling
R-BTA-983168 Antigen processing: Ubiquitination & Proteasome degradation

Protein family/group databases

MEROPSiT01.973

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit alpha type-4 (EC:3.4.25.1By similarity)
Gene namesi
Name:PSMA4
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 21

Organism-specific databases

VGNCiVGNC:33439 PSMA4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002740321 – 261Proteasome subunit alpha type-4Add BLAST261

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei13PhosphoserineBy similarity1
Modified residuei75PhosphoserineBy similarity1
Modified residuei127N6-acetyllysineBy similarity1
Modified residuei173PhosphoserineBy similarity1
Modified residuei176N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ3ZCK9
PRIDEiQ3ZCK9

Expressioni

Gene expression databases

BgeeiENSBTAG00000014440

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is a barrel-shaped complex made of 28 subunits that are arranged in four stacked rings. The two outer rings are each formed by seven alpha subunits, and the two inner rings are formed by seven beta subunits. The proteolytic activity is exerted by three beta-subunits PSMB5, PSMB6 and PSMB7 (PubMed:12015144).1 Publication

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000019203

Structurei

Secondary structure

1261
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni3 – 5Combined sources3
Helixi19 – 28Combined sources10
Beta strandi34 – 38Combined sources5
Beta strandi40 – 47Combined sources8
Beta strandi63 – 67Combined sources5
Beta strandi69 – 78Combined sources10
Helixi80 – 101Combined sources22
Helixi107 – 120Combined sources14
Turni121 – 123Combined sources3
Beta strandi124 – 126Combined sources3
Beta strandi132 – 140Combined sources9
Turni141 – 143Combined sources3
Beta strandi144 – 151Combined sources8
Turni152 – 154Combined sources3
Beta strandi155 – 158Combined sources4
Beta strandi160 – 165Combined sources6
Turni166 – 169Combined sources4
Helixi170 – 178Combined sources9
Helixi186 – 199Combined sources14
Beta strandi200 – 202Combined sources3
Beta strandi212 – 217Combined sources6
Beta strandi220 – 222Combined sources3
Beta strandi224 – 227Combined sources4
Helixi230 – 241Combined sources12
Turni245 – 247Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IRUX-ray2.75C/Q1-261[»]
ProteinModelPortaliQ3ZCK9
SMRiQ3ZCK9
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ3ZCK9

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1A family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0178 Eukaryota
COG0638 LUCA
GeneTreeiENSGT00550000074827
HOGENOMiHOG000091085
HOVERGENiHBG003005
InParanoidiQ3ZCK9
KOiK02728
OMAiMSKTMDS
OrthoDBiEOG091G0F2L
TreeFamiTF106209

Family and domain databases

Gene3Di3.60.20.10, 1 hit
InterProiView protein in InterPro
IPR029055 Ntn_hydrolases_N
IPR023332 Proteasome_alpha-type
IPR000426 Proteasome_asu_N
IPR016050 Proteasome_bsu_CS
IPR001353 Proteasome_sua/b
IPR034647 Proteasome_subunit_alpha4
PANTHERiPTHR11599:SF13 PTHR11599:SF13, 1 hit
PfamiView protein in Pfam
PF00227 Proteasome, 1 hit
PF10584 Proteasome_A_N, 1 hit
SMARTiView protein in SMART
SM00948 Proteasome_A_N, 1 hit
SUPFAMiSSF56235 SSF56235, 1 hit
PROSITEiView protein in PROSITE
PS00388 PROTEASOME_ALPHA_1, 1 hit
PS51475 PROTEASOME_ALPHA_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q3ZCK9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRRYDSRTT IFSPEGRLYQ VEYAMEAIGH AGTCLGILAN DGVLLAAERR
60 70 80 90 100
NIHKLLDEVF FSEKIYKLNE DMACSVAGIT SDANVLTNEL RLIAQRYLLQ
110 120 130 140 150
YQEPIPCEQL VTALCDIKQA YTQFGGKRPF GVSLLYIGWD KHYGFQLYQS
160 170 180 190 200
DPSGNYGGWK ATCIGNNSAA AVSMLKQDYK EGEMTLKSAL ALAIKVLNKT
210 220 230 240 250
MDVSKLSAEK VEIATLTREN GKTVIRVLKQ KEVEQLIKKH EEEEAKAERE
260
KKEKEQKEKD K
Length:261
Mass (Da):29,484
Last modified:September 27, 2005 - v1
Checksum:i7867422B1B31F3B9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC102102 mRNA Translation: AAI02103.1
RefSeqiNP_001029553.1, NM_001034381.2
UniGeneiBt.49419

Genome annotation databases

EnsembliENSBTAT00000019203; ENSBTAP00000019203; ENSBTAG00000014440
GeneIDi510423
KEGGibta:510423

Similar proteinsi

Entry informationi

Entry nameiPSA4_BOVIN
AccessioniPrimary (citable) accession number: Q3ZCK9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: September 27, 2005
Last modified: May 23, 2018
This is version 110 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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