ID   BPNT1_BOVIN             Reviewed;         308 AA.
AC   Q3ZCK3; A7E3X5;
DT   03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   27-SEP-2005, sequence version 1.
DT   16-JUN-2009, entry version 30.
DE   RecName: Full=3'(2'),5'-bisphosphate nucleotidase 1;
DE            EC=3.1.3.7;
DE   AltName: Full=Bisphosphate 3'-nucleotidase 1;
GN   Name=BPNT1;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia;
OC   Pecora; Bovidae; Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=16305752; DOI=10.1186/1471-2164-6-166;
RA   Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L.,
RA   Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.;
RT   "Characterization of 954 bovine full-CDS cDNA sequences.";
RL   BMC Genomics 6:166-166(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Crossbred X Angus; TISSUE=Ileum;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Converts adenosine 3'-phosphate 5'-phosphosulfate (PAPS)
CC       to adenosine 5'-phosphosulfate (APS) and 3'(2')-phosphoadenosine
CC       5'- phosphate (PAP) to AMP. Has 1000-fold lower activity towards
CC       inositol 1,4-bisphosphate (Ins(1,4)P2) and inositol 1,3,4-
CC       trisphosphate (Ins(1,3,4)P3), but does not hydrolyze Ins(1)P,
CC       Ins(3,4)P2, Ins(1,3,4,5)P4 or InsP6 (By similarity).
CC   -!- CATALYTIC ACTIVITY: Adenosine 3',5'-bisphosphate + H(2)O =
CC       adenosine 5'-phosphate + phosphate.
CC   -!- COFACTOR: Magnesium (By similarity).
CC   -!- ENZYME REGULATION: Uncompetitive inhibition by micromolar
CC       concentrations of lithium. Competitive inhibition by inositol 1,4-
CC       bisphosphate (By similarity).
CC   -!- SIMILARITY: Belongs to the inositol monophosphatase family.
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DR   EMBL; BT030746; ABS45062.1; -; mRNA.
DR   EMBL; BC102110; AAI02111.1; -; mRNA.
DR   IPI; IPI00687550; -.
DR   RefSeq; NP_001029711.1; -.
DR   UniGene; Bt.49571; -.
DR   SMR; Q3ZCK3; 7-308.
DR   Ensembl; ENSBTAG00000004064; Bos taurus.
DR   GeneID; 521254; -.
DR   KEGG; bta:521254; -.
DR   HOVERGEN; Q3ZCK3; -.
DR   OMA; Q3ZCK3; GFQLKEA.
DR   BRENDA; 3.1.3.7; 251.
DR   GO; GO:0008441; F:3'(2'),5'-bisphosphate nucleotidase activity; IEA:EC.
DR   GO; GO:0004437; F:inositol or phosphatidylinositol phosphatas...; IEA:InterPro.
DR   GO; GO:0031403; F:lithium ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-KW.
DR   InterPro; IPR000760; Inositol_P.
DR   PANTHER; PTHR20854; Inositol_P; 1.
DR   Pfam; PF00459; Inositol_P; 1.
DR   PRINTS; PR00378; INOSPHPHTASE.
DR   PROSITE; PS00629; IMP_1; 1.
DR   PROSITE; PS00630; IMP_2; 1.
PE   2: Evidence at transcript level;
KW   Acetylation; Hydrolase; Lithium; Magnesium; Metal-binding.
FT   INIT_MET      1      1       Removed (By similarity).
FT   CHAIN         2    308       3'(2'),5'-bisphosphate nucleotidase 1.
FT                                /FTId=PRO_0000282934.
FT   REGION      195    198       Substrate binding (By similarity).
FT   METAL        74     74       Magnesium 1 (By similarity).
FT   METAL       117    117       Magnesium 1 (By similarity).
FT   METAL       117    117       Magnesium 2 (By similarity).
FT   METAL       119    119       Magnesium 1; via carbonyl oxygen (By
FT                                similarity).
FT   METAL       120    120       Magnesium 2 (By similarity).
FT   METAL       247    247       Magnesium 2 (By similarity).
FT   MOD_RES       2      2       N-acetylalanine (By similarity).
SQ   SEQUENCE   308 AA;  33328 MW;  8A9184010B4B893C CRC64;
     MASSPTVLMR LVASAYSIAQ KAGTIVRRVI AEGDLGIIEK TCATDLQTKA DRLVQVSICS
     SLARKFPKLT IIGEEDLPPE DVDQELIEDG QWEEILKQPC PSQYSAIKEE DLVVWVDPLD
     GTKEYTEGLL DNVTVLIGIA YEGKAIAGVI NQPYYNYQAG PDAVLGRTIW GVLGLGAFGF
     QLKEAPAGKH IITTTRSHNS QLVTDCITAM NPDDVLRVGG AGNKIIQLIE GKASAYVFAS
     PGCKKWDTCA PEVILHAVGG KLTDIHGNAL QYNKEVKHMN SAGVLATLRN YDYYASRVPQ
     SVKNALVP
//