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Protein

RNA-binding protein 8A

Gene

RBM8A

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC key regulator PYM1 leading to EJC disassembly in the cytoplasm and translation enhancement of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Its removal from cytoplasmic mRNAs requires translation initiation from EJC-bearing spliced mRNAs. Associates preferentially with mRNAs produced by splicing. Does not interact with pre-mRNAs, introns, or mRNAs produced from intronless cDNAs. Associates with both nuclear mRNAs and newly exported cytoplasmic mRNAs. The MAGOH-RBM8A heterodimer is a component of the nonsense mediated decay (NMD) pathway. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms; the function is different from the established EJC assembly (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing, mRNA transport, Nonsense-mediated mRNA decay, Translation regulation, Transport

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-BTA-109688. Cleavage of Growing Transcript in the Termination Region.
R-BTA-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-BTA-72163. mRNA Splicing - Major Pathway.
R-BTA-72187. mRNA 3'-end processing.
R-BTA-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-binding protein 8A
Alternative name(s):
RNA-binding motif protein 8A
Gene namesi
Name:RBM8A
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 3

Subcellular locationi

  • Nucleus By similarity
  • Nucleus speckle By similarity
  • Cytoplasm By similarity

  • Note: Nucleocytoplasmic shuttling protein. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Colocalizes with the core EJC, ALYREF/THOC4, NXF1 and UAP56 in the nucleus and nuclear speckles (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002826052 – 174RNA-binding protein 8AAdd BLAST173

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei24PhosphoserineBy similarity1
Cross-linki27Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei42PhosphoserineBy similarity1
Modified residuei56PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ3ZCE8.
PeptideAtlasiQ3ZCE8.
PRIDEiQ3ZCE8.

Expressioni

Gene expression databases

BgeeiENSBTAG00000008369.

Interactioni

Subunit structurei

Heterodimer with RBM8A. Part of the mRNA splicing-dependent exon junction complex (EJC) complex; the core complex contains CASC3, EIF4A3, MAGOH and RBM8A. Interacts with PYM1; the interaction is direct and dissociates the EJC from spliced mRNAs. Found in a post-splicing complex with NXF1, RBM8A, UPF1, UPF2, UPF3A, UPF3B and RNPS1. Interacts with BAT1, MAGOH, OVCA1, UPF3B, RNPS1, SRRM1 and ALYREF/THOC4. Interacts with IPO13; the interaction mediates the nuclear import of the MAGOH-RBM8A heterodimer. Identified in the spliceosome C complex. Associates with polysomes (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000011016.

Structurei

3D structure databases

ProteinModelPortaliQ3ZCE8.
SMRiQ3ZCE8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini73 – 151RRMPROSITE-ProRule annotationAdd BLAST79

Sequence similaritiesi

Belongs to the RBM8A family.Curated
Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0130. Eukaryota.
ENOG4111IHM. LUCA.
GeneTreeiENSGT00730000111185.
HOGENOMiHOG000183826.
HOVERGENiHBG055173.
InParanoidiQ3ZCE8.
KOiK12876.
OMAiISVDWGF.
OrthoDBiEOG091G0PLI.
TreeFamiTF314933.

Family and domain databases

CDDicd12324. RRM_RBM8. 1 hit.
Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR008111. RNA-bd_8.
IPR000504. RRM_dom.
IPR033744. RRM_RBM8.
[Graphical view]
PANTHERiPTHR23139:SF72. PTHR23139:SF72. 1 hit.
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
PRINTSiPR01738. RNABINDINGM8.
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3ZCE8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADVLDLHEA GGEDFAMDED GDESIHKLKE KAKKRKGRGF GSEEGSRARM
60 70 80 90 100
REDYDSVEQD GDEPGPQRSV EGWILFVTGV HEEATEEDIH DKFAEYGEIK
110 120 130 140 150
NIHLNLDRRT GYLKGYTLVE YETYKEAQAA MEGLNGQDLM GQPISVDWCF
160 170
VRGPPKGKRR GGRRRSRSPD RRRR
Length:174
Mass (Da):19,889
Last modified:July 7, 2009 - v2
Checksum:i70BBD03CDDFEECFE
GO
Isoform 2 (identifier: Q3ZCE8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     43-43: Missing.

Show »
Length:173
Mass (Da):19,760
Checksum:i6710C1BD9CFAF92E
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03760043Missing in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT025376 mRNA. Translation: ABF57332.1.
BC102484 mRNA. Translation: AAI02485.1.
RefSeqiNP_001030401.1. NM_001035324.2. [Q3ZCE8-2]
XP_015318708.1. XM_015463222.1. [Q3ZCE8-1]
UniGeneiBt.49450.

Genome annotation databases

EnsembliENSBTAT00000011016; ENSBTAP00000011016; ENSBTAG00000008369. [Q3ZCE8-2]
GeneIDi518045.
KEGGibta:518045.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT025376 mRNA. Translation: ABF57332.1.
BC102484 mRNA. Translation: AAI02485.1.
RefSeqiNP_001030401.1. NM_001035324.2. [Q3ZCE8-2]
XP_015318708.1. XM_015463222.1. [Q3ZCE8-1]
UniGeneiBt.49450.

3D structure databases

ProteinModelPortaliQ3ZCE8.
SMRiQ3ZCE8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000011016.

Proteomic databases

PaxDbiQ3ZCE8.
PeptideAtlasiQ3ZCE8.
PRIDEiQ3ZCE8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000011016; ENSBTAP00000011016; ENSBTAG00000008369. [Q3ZCE8-2]
GeneIDi518045.
KEGGibta:518045.

Organism-specific databases

CTDi9939.

Phylogenomic databases

eggNOGiKOG0130. Eukaryota.
ENOG4111IHM. LUCA.
GeneTreeiENSGT00730000111185.
HOGENOMiHOG000183826.
HOVERGENiHBG055173.
InParanoidiQ3ZCE8.
KOiK12876.
OMAiISVDWGF.
OrthoDBiEOG091G0PLI.
TreeFamiTF314933.

Enzyme and pathway databases

ReactomeiR-BTA-109688. Cleavage of Growing Transcript in the Termination Region.
R-BTA-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-BTA-72163. mRNA Splicing - Major Pathway.
R-BTA-72187. mRNA 3'-end processing.
R-BTA-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Gene expression databases

BgeeiENSBTAG00000008369.

Family and domain databases

CDDicd12324. RRM_RBM8. 1 hit.
Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR008111. RNA-bd_8.
IPR000504. RRM_dom.
IPR033744. RRM_RBM8.
[Graphical view]
PANTHERiPTHR23139:SF72. PTHR23139:SF72. 1 hit.
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
PRINTSiPR01738. RNABINDINGM8.
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRBM8A_BOVIN
AccessioniPrimary (citable) accession number: Q3ZCE8
Secondary accession number(s): Q1JPH7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: July 7, 2009
Last modified: November 30, 2016
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.