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Q3ZC89

- MAP2_BOVIN

UniProt

Q3ZC89 - MAP2_BOVIN

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Protein

Methionine aminopeptidase 2

Gene
METAP2
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val) By similarity.UniRule annotation
Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis By similarity.UniRule annotation

Catalytic activityi

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

Cofactori

Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei230 – 2301Substrate By similarity
Metal bindingi250 – 2501Divalent metal cation 1 By similarity
Metal bindingi261 – 2611Divalent metal cation 1 By similarity
Metal bindingi261 – 2611Divalent metal cation 2; catalytic By similarity
Metal bindingi330 – 3301Divalent metal cation 2; catalytic; via tele nitrogen By similarity
Binding sitei338 – 3381Substrate By similarity
Metal bindingi363 – 3631Divalent metal cation 2; catalytic By similarity
Metal bindingi458 – 4581Divalent metal cation 1 By similarity
Metal bindingi458 – 4581Divalent metal cation 2; catalytic By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-HAMAP
  2. metalloaminopeptidase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. protein initiator methionine removal Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Keywords - Ligandi

Metal-binding

Protein family/group databases

MEROPSiM24.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine aminopeptidase 2 (EC:3.4.11.18)
Short name:
MAP 2
Short name:
MetAP 2
Alternative name(s):
Initiation factor 2-associated 67 kDa glycoprotein
Short name:
p67
Short name:
p67eIF2
Peptidase M
Gene namesi
Name:METAP2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

Cytoplasm
Note: About 30% of expressed METAP2 associates with polysomes By similarity.UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 477476Methionine aminopeptidase 2UniRule annotationPRO_0000246091Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine By similarity
Modified residuei45 – 451Phosphoserine By similarity
Modified residuei62 – 621Phosphoserine; alternate By similarity
Glycosylationi62 – 621O-linked (GlcNAc); alternate By similarity

Post-translational modificationi

Contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. O-glycosylation is required for EIF2S1 binding By similarity.UniRule annotation

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ3ZC89.
PRIDEiQ3ZC89.

Interactioni

Subunit structurei

Binds EIF2S1 at low magnesium concentrations. Interacts strongly with the eIF-2 gamma-subunit EIF2S3 By similarity.

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000026568.

Structurei

3D structure databases

ProteinModelPortaliQ3ZC89.
SMRiQ3ZC89. Positions 109-477.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi36 – 10974Lys-richUniRule annotationAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0024.
HOGENOMiHOG000226278.
HOVERGENiHBG050495.
InParanoidiQ3ZC89.
KOiK01265.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.90.230.10. 2 hits.
HAMAPiMF_03175. MetAP_2_euk.
InterProiIPR001714. Pept_M24_MAP.
IPR000994. Pept_M24_structural-domain.
IPR002468. Pept_M24A_MAP2.
IPR018349. Pept_M24A_MAP2_BS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00557. Peptidase_M24. 1 hit.
[Graphical view]
PRINTSiPR00599. MAPEPTIDASE.
SUPFAMiSSF55920. SSF55920. 2 hits.
TIGRFAMsiTIGR00501. met_pdase_II. 1 hit.
PROSITEiPS01202. MAP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3ZC89-1 [UniParc]FASTAAdd to Basket

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MAGVEEAASC GSHLNGDLDP DEREEGAAST AEEAAKKKKR KKKKSKGAAT    50
GQQEPDKEAG ASVDEVTRQL ERQALEEKEK DDDDEDGDGD GDGATGKKKK 100
KKKKKRGPKV QTDPPSVPIC DLYPNGVFPK GQECEYPPTQ DGRTAAWRTT 150
SEEKKALDQA SEEIWNDFRE AAEAHRQVRK YVMSWIKPGM TMIEICEKME 200
DCSRKLIKEN GLNAGLAFPT GCSLNNCAAH YTPNAGDTTV LQYDDICKID 250
FGTHISGRII DCAFTVTFNP KYDTLLKAVK DATNTGIKCA GIDVRLCDVG 300
EAIQEVMESY EVEIDGKTYQ VKPIRNLNGH SIGPYRIHAG KTVPIVKGGE 350
ATRMEEGEVY AIETFGSTGK GVVHDDMECS HYMKNFDVGH VPIRLPRTKH 400
LLNVINENFG TLAFCRRWLD RLGESKYLMA LKNLCDLGIV DPYPPLCDIK 450
GSYTAQFEHT ILLRPTCKEV VSRGDDY 477
Length:477
Mass (Da):52,812
Last modified:September 27, 2005 - v1
Checksum:i4D10309613D2F0F1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC102824 mRNA. Translation: AAI02825.1.
RefSeqiNP_001035583.1. NM_001040493.1.
UniGeneiBt.49424.

Genome annotation databases

GeneIDi404150.
KEGGibta:404150.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC102824 mRNA. Translation: AAI02825.1 .
RefSeqi NP_001035583.1. NM_001040493.1.
UniGenei Bt.49424.

3D structure databases

ProteinModelPortali Q3ZC89.
SMRi Q3ZC89. Positions 109-477.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000026568.

Protein family/group databases

MEROPSi M24.002.

Proteomic databases

PaxDbi Q3ZC89.
PRIDEi Q3ZC89.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 404150.
KEGGi bta:404150.

Organism-specific databases

CTDi 10988.

Phylogenomic databases

eggNOGi COG0024.
HOGENOMi HOG000226278.
HOVERGENi HBG050495.
InParanoidi Q3ZC89.
KOi K01265.

Miscellaneous databases

NextBioi 20817584.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
3.90.230.10. 2 hits.
HAMAPi MF_03175. MetAP_2_euk.
InterProi IPR001714. Pept_M24_MAP.
IPR000994. Pept_M24_structural-domain.
IPR002468. Pept_M24A_MAP2.
IPR018349. Pept_M24A_MAP2_BS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF00557. Peptidase_M24. 1 hit.
[Graphical view ]
PRINTSi PR00599. MAPEPTIDASE.
SUPFAMi SSF55920. SSF55920. 2 hits.
TIGRFAMsi TIGR00501. met_pdase_II. 1 hit.
PROSITEi PS01202. MAP_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Ileum.

Entry informationi

Entry nameiMAP2_BOVIN
AccessioniPrimary (citable) accession number: Q3ZC89
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: September 27, 2005
Last modified: June 11, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi