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Q3ZC89

- MAP2_BOVIN

UniProt

Q3ZC89 - MAP2_BOVIN

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Protein

Methionine aminopeptidase 2

Gene

METAP2

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).UniRule annotation
Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis.UniRule annotation

Catalytic activityi

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

Cofactori

Co2+UniRule annotation, Zn2+UniRule annotation, Mn2+UniRule annotation, Fe2+UniRule annotationNote: Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei230 – 2301SubstrateUniRule annotation
Metal bindingi250 – 2501Divalent metal cation 1UniRule annotation
Metal bindingi261 – 2611Divalent metal cation 1UniRule annotation
Metal bindingi261 – 2611Divalent metal cation 2; catalyticUniRule annotation
Metal bindingi330 – 3301Divalent metal cation 2; catalytic; via tele nitrogenUniRule annotation
Binding sitei338 – 3381SubstrateUniRule annotation
Metal bindingi363 – 3631Divalent metal cation 2; catalyticUniRule annotation
Metal bindingi458 – 4581Divalent metal cation 1UniRule annotation
Metal bindingi458 – 4581Divalent metal cation 2; catalyticUniRule annotation

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-HAMAP
  2. metalloaminopeptidase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. protein initiator methionine removal Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Keywords - Ligandi

Metal-binding

Protein family/group databases

MEROPSiM24.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine aminopeptidase 2UniRule annotation (EC:3.4.11.18UniRule annotation)
Short name:
MAP 2UniRule annotation
Short name:
MetAP 2UniRule annotation
Alternative name(s):
Initiation factor 2-associated 67 kDa glycoproteinUniRule annotation
Short name:
p67UniRule annotation
Short name:
p67eIF2UniRule annotation
Peptidase MUniRule annotation
Gene namesi
Name:METAP2UniRule annotation
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

Cytoplasm
Note: About 30% of expressed METAP2 associates with polysomes.UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 477476Methionine aminopeptidase 2PRO_0000246091Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei45 – 451PhosphoserineBy similarity
Modified residuei62 – 621Phosphoserine; alternateBy similarity
Glycosylationi62 – 621O-linked (GlcNAc); alternateBy similarity

Post-translational modificationi

Contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. O-glycosylation is required for EIF2S1 binding.UniRule annotation

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ3ZC89.
PRIDEiQ3ZC89.

Interactioni

Subunit structurei

Binds EIF2S1 at low magnesium concentrations. Interacts strongly with the eIF-2 gamma-subunit EIF2S3.UniRule annotation

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000026568.

Structurei

3D structure databases

ProteinModelPortaliQ3ZC89.
SMRiQ3ZC89. Positions 109-477.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi36 – 10974Lys-richAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0024.
HOGENOMiHOG000226278.
HOVERGENiHBG050495.
InParanoidiQ3ZC89.
KOiK01265.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.90.230.10. 2 hits.
HAMAPiMF_03175. MetAP_2_euk.
InterProiIPR001714. Pept_M24_MAP.
IPR000994. Pept_M24_structural-domain.
IPR002468. Pept_M24A_MAP2.
IPR018349. Pept_M24A_MAP2_BS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00557. Peptidase_M24. 1 hit.
[Graphical view]
PRINTSiPR00599. MAPEPTIDASE.
SUPFAMiSSF55920. SSF55920. 2 hits.
TIGRFAMsiTIGR00501. met_pdase_II. 1 hit.
PROSITEiPS01202. MAP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3ZC89-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAGVEEAASC GSHLNGDLDP DEREEGAAST AEEAAKKKKR KKKKSKGAAT
60 70 80 90 100
GQQEPDKEAG ASVDEVTRQL ERQALEEKEK DDDDEDGDGD GDGATGKKKK
110 120 130 140 150
KKKKKRGPKV QTDPPSVPIC DLYPNGVFPK GQECEYPPTQ DGRTAAWRTT
160 170 180 190 200
SEEKKALDQA SEEIWNDFRE AAEAHRQVRK YVMSWIKPGM TMIEICEKME
210 220 230 240 250
DCSRKLIKEN GLNAGLAFPT GCSLNNCAAH YTPNAGDTTV LQYDDICKID
260 270 280 290 300
FGTHISGRII DCAFTVTFNP KYDTLLKAVK DATNTGIKCA GIDVRLCDVG
310 320 330 340 350
EAIQEVMESY EVEIDGKTYQ VKPIRNLNGH SIGPYRIHAG KTVPIVKGGE
360 370 380 390 400
ATRMEEGEVY AIETFGSTGK GVVHDDMECS HYMKNFDVGH VPIRLPRTKH
410 420 430 440 450
LLNVINENFG TLAFCRRWLD RLGESKYLMA LKNLCDLGIV DPYPPLCDIK
460 470
GSYTAQFEHT ILLRPTCKEV VSRGDDY
Length:477
Mass (Da):52,812
Last modified:September 27, 2005 - v1
Checksum:i4D10309613D2F0F1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC102824 mRNA. Translation: AAI02825.1.
RefSeqiNP_001035583.1. NM_001040493.1.
UniGeneiBt.49424.

Genome annotation databases

GeneIDi404150.
KEGGibta:404150.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC102824 mRNA. Translation: AAI02825.1 .
RefSeqi NP_001035583.1. NM_001040493.1.
UniGenei Bt.49424.

3D structure databases

ProteinModelPortali Q3ZC89.
SMRi Q3ZC89. Positions 109-477.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000026568.

Protein family/group databases

MEROPSi M24.002.

Proteomic databases

PaxDbi Q3ZC89.
PRIDEi Q3ZC89.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 404150.
KEGGi bta:404150.

Organism-specific databases

CTDi 10988.

Phylogenomic databases

eggNOGi COG0024.
HOGENOMi HOG000226278.
HOVERGENi HBG050495.
InParanoidi Q3ZC89.
KOi K01265.

Miscellaneous databases

NextBioi 20817584.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
3.90.230.10. 2 hits.
HAMAPi MF_03175. MetAP_2_euk.
InterProi IPR001714. Pept_M24_MAP.
IPR000994. Pept_M24_structural-domain.
IPR002468. Pept_M24A_MAP2.
IPR018349. Pept_M24A_MAP2_BS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF00557. Peptidase_M24. 1 hit.
[Graphical view ]
PRINTSi PR00599. MAPEPTIDASE.
SUPFAMi SSF55920. SSF55920. 2 hits.
TIGRFAMsi TIGR00501. met_pdase_II. 1 hit.
PROSITEi PS01202. MAP_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Ileum.

Entry informationi

Entry nameiMAP2_BOVIN
AccessioniPrimary (citable) accession number: Q3ZC89
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: September 27, 2005
Last modified: November 26, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3