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Protein

L-gulonolactone oxidase

Gene

GULO

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Oxidizes L-gulono-1,4-lactone to hydrogen peroxide and L-xylo-hexulonolactone which spontaneously isomerizes to L-ascorbate.By similarity

Catalytic activityi

L-gulono-1,4-lactone + O2 = L-ascorbate + H2O2.

Cofactori

FADBy similarity

Pathwayi: L-ascorbate biosynthesis via UDP-alpha-D-glucuronate pathway

This protein is involved in step 4 of the subpathway that synthesizes L-ascorbate from UDP-alpha-D-glucuronate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. Regucalcin (RGN)
  4. L-gulonolactone oxidase (GULO)
This subpathway is part of the pathway L-ascorbate biosynthesis via UDP-alpha-D-glucuronate pathway, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-ascorbate from UDP-alpha-D-glucuronate, the pathway L-ascorbate biosynthesis via UDP-alpha-D-glucuronate pathway and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAscorbate biosynthesis
LigandFAD, Flavoprotein

Enzyme and pathway databases

UniPathwayiUPA00991; UER00939

Names & Taxonomyi

Protein namesi
Recommended name:
L-gulonolactone oxidase (EC:1.1.3.8)
Short name:
LGO
Alternative name(s):
L-gulono-gamma-lactone oxidase
Short name:
GLO
Gene namesi
Name:GULO
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei253 – 273HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002316731 – 440L-gulonolactone oxidaseAdd BLAST440

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei54Pros-8alpha-FAD histidineBy similarity1

Proteomic databases

PaxDbiQ3ZC33
PRIDEiQ3ZC33

Expressioni

Gene expression databases

BgeeiENSBTAG00000026748

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000037993

Structurei

3D structure databases

ProteinModelPortaliQ3ZC33
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini17 – 187FAD-binding PCMH-typePROSITE-ProRule annotationAdd BLAST171

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4730 Eukaryota
COG0277 LUCA
GeneTreeiENSGT00510000049722
HOGENOMiHOG000252847
HOVERGENiHBG005834
InParanoidiQ3ZC33
KOiK00103
OMAiFRFLWFP
OrthoDBiEOG091G0FC4
TreeFamiTF328994

Family and domain databases

Gene3Di3.30.43.10, 1 hit
InterProiView protein in InterPro
IPR007173 ALO
IPR016166 FAD-bd_2
IPR036318 FAD-bd_2-like_sf
IPR016167 FAD-bd_2_sub1
IPR010031 FAD_lactone_oxidase
IPR006094 Oxid_FAD_bind_N
IPR006093 Oxy_OxRdtase_FAD_BS
IPR030654 Sugar_lactone_oxidase
PANTHERiPTHR43762 PTHR43762, 1 hit
PfamiView protein in Pfam
PF04030 ALO, 1 hit
PF01565 FAD_binding_4, 1 hit
PIRSFiPIRSF000136 LGO_GLO, 1 hit
SUPFAMiSSF56176 SSF56176, 1 hit
TIGRFAMsiTIGR01678 FAD_lactone_ox, 1 hit
PROSITEiView protein in PROSITE
PS51387 FAD_PCMH, 1 hit
PS00862 OX2_COVAL_FAD, 1 hit

Sequencei

Sequence statusi: Complete.

Q3ZC33-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVHGYKGVKF QNWARTYGCC PEMYFQPTSV EEVREVLALA RQQNKRVKVV
60 70 80 90 100
GGGHSPSDIA CTDGFMIHMG KMNRVLKVDT EKKQVTVEAG ILLADLHPQL
110 120 130 140 150
DKHGLALSNL GAVSDVTAGG VIGSGTHNTG IKHGILATQV VALTLLTANG
160 170 180 190 200
TILECSESSN AEVFQAARVH LGCLGVILTV TLQCVPQFHL QETTFPSTLK
210 220 230 240 250
EVLDNLDSHL KKSEYFRFLW FPHSENVSVI YQDHTNKPPS SSANWFWDYA
260 270 280 290 300
IGFYLLEFLL WISTFLPGLV GWINRFFFWL LFNGKKENCN LSHKIFTYEC
310 320 330 340 350
RFKQHVQDWA IPREKTKEAL LELKAMLEAN PKVVAHYPVE VRFTRGDDIL
360 370 380 390 400
LSPCFQRDSC YMNIIMYRPY GKDVPRLDYW LAYETIMKKV GGRPHWAKAH
410 420 430 440
NCTRKDFEKM YPAFQRFCAI REKLDPTGMF LNAYLEKVFY
Length:440
Mass (Da):50,282
Last modified:January 23, 2007 - v3
Checksum:i8AAEE8413A80F048
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC102936 mRNA Translation: AAI02937.1
RefSeqiNP_001029215.1, NM_001034043.2
UniGeneiBt.49608

Genome annotation databases

EnsembliENSBTAT00000038177; ENSBTAP00000037993; ENSBTAG00000026748
GeneIDi286812
KEGGibta:286812

Similar proteinsi

Entry informationi

Entry nameiGGLO_BOVIN
AccessioniPrimary (citable) accession number: Q3ZC33
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 106 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
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