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Protein

L-gulonolactone oxidase

Gene

GULO

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Oxidizes L-gulono-1,4-lactone to hydrogen peroxide and L-xylo-hexulonolactone which spontaneously isomerizes to L-ascorbate.By similarity

Catalytic activityi

L-gulono-1,4-lactone + O2 = L-ascorbate + H2O2.

Cofactori

FADBy similarity

Pathwayi: L-ascorbate biosynthesis via UDP-alpha-D-glucuronate pathway

This protein is involved in step 4 of the subpathway that synthesizes L-ascorbate from UDP-alpha-D-glucuronate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. Regucalcin (RGN)
  4. L-gulonolactone oxidase (GULO)
This subpathway is part of the pathway L-ascorbate biosynthesis via UDP-alpha-D-glucuronate pathway, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-ascorbate from UDP-alpha-D-glucuronate, the pathway L-ascorbate biosynthesis via UDP-alpha-D-glucuronate pathway and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAscorbate biosynthesis
LigandFAD, Flavoprotein

Enzyme and pathway databases

UniPathwayiUPA00991; UER00939.

Names & Taxonomyi

Protein namesi
Recommended name:
L-gulonolactone oxidase (EC:1.1.3.8)
Short name:
LGO
Alternative name(s):
L-gulono-gamma-lactone oxidase
Short name:
GLO
Gene namesi
Name:GULO
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei253 – 273HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002316731 – 440L-gulonolactone oxidaseAdd BLAST440

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei54Pros-8alpha-FAD histidineBy similarity1

Proteomic databases

PaxDbiQ3ZC33.
PRIDEiQ3ZC33.

Expressioni

Gene expression databases

BgeeiENSBTAG00000026748.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000037993.

Structurei

3D structure databases

ProteinModelPortaliQ3ZC33.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini17 – 187FAD-binding PCMH-typePROSITE-ProRule annotationAdd BLAST171

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4730. Eukaryota.
COG0277. LUCA.
GeneTreeiENSGT00510000049722.
HOGENOMiHOG000252847.
HOVERGENiHBG005834.
InParanoidiQ3ZC33.
KOiK00103.
OMAiFRFLWFP.
OrthoDBiEOG091G0FC4.
TreeFamiTF328994.

Family and domain databases

Gene3Di3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
3.40.462.10. 1 hit.
InterProiView protein in InterPro
IPR007173. ALO.
IPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR036318. FAD-bd_2-like_sf.
IPR016167. FAD-bd_2_sub1.
IPR010031. FAD_lactone_oxidase.
IPR006094. Oxid_FAD_bind_N.
IPR006093. Oxy_OxRdtase_FAD_BS.
IPR030654. Sugar_lactone_oxidase.
IPR016170. V_Alc_oxidase/Cytok_DH_C_sf.
PANTHERiPTHR43762. PTHR43762. 1 hit.
PfamiView protein in Pfam
PF04030. ALO. 1 hit.
PF01565. FAD_binding_4. 1 hit.
PIRSFiPIRSF000136. LGO_GLO. 1 hit.
SUPFAMiSSF56176. SSF56176. 1 hit.
TIGRFAMsiTIGR01678. FAD_lactone_ox. 1 hit.
PROSITEiView protein in PROSITE
PS51387. FAD_PCMH. 1 hit.
PS00862. OX2_COVAL_FAD. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3ZC33-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVHGYKGVKF QNWARTYGCC PEMYFQPTSV EEVREVLALA RQQNKRVKVV
60 70 80 90 100
GGGHSPSDIA CTDGFMIHMG KMNRVLKVDT EKKQVTVEAG ILLADLHPQL
110 120 130 140 150
DKHGLALSNL GAVSDVTAGG VIGSGTHNTG IKHGILATQV VALTLLTANG
160 170 180 190 200
TILECSESSN AEVFQAARVH LGCLGVILTV TLQCVPQFHL QETTFPSTLK
210 220 230 240 250
EVLDNLDSHL KKSEYFRFLW FPHSENVSVI YQDHTNKPPS SSANWFWDYA
260 270 280 290 300
IGFYLLEFLL WISTFLPGLV GWINRFFFWL LFNGKKENCN LSHKIFTYEC
310 320 330 340 350
RFKQHVQDWA IPREKTKEAL LELKAMLEAN PKVVAHYPVE VRFTRGDDIL
360 370 380 390 400
LSPCFQRDSC YMNIIMYRPY GKDVPRLDYW LAYETIMKKV GGRPHWAKAH
410 420 430 440
NCTRKDFEKM YPAFQRFCAI REKLDPTGMF LNAYLEKVFY
Length:440
Mass (Da):50,282
Last modified:January 23, 2007 - v3
Checksum:i8AAEE8413A80F048
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC102936 mRNA. Translation: AAI02937.1.
RefSeqiNP_001029215.1. NM_001034043.2.
UniGeneiBt.49608.

Genome annotation databases

EnsembliENSBTAT00000038177; ENSBTAP00000037993; ENSBTAG00000026748.
GeneIDi286812.
KEGGibta:286812.

Similar proteinsi

Entry informationi

Entry nameiGGLO_BOVIN
AccessioniPrimary (citable) accession number: Q3ZC33
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: January 23, 2007
Last modified: November 22, 2017
This is version 104 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families