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Protein

L-gulonolactone oxidase

Gene

GULO

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Oxidizes L-gulono-1,4-lactone to hydrogen peroxide and L-xylo-hexulonolactone which spontaneously isomerizes to L-ascorbate.By similarity

Catalytic activityi

L-gulono-1,4-lactone + O2 = L-ascorbate + H2O2.

Cofactori

FADBy similarity

Pathwayi: L-ascorbate biosynthesis via UDP-alpha-D-glucuronate pathway

This protein is involved in step 4 of the subpathway that synthesizes L-ascorbate from UDP-alpha-D-glucuronate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. Regucalcin (RGN)
  4. L-gulonolactone oxidase (GULO)
This subpathway is part of the pathway L-ascorbate biosynthesis via UDP-alpha-D-glucuronate pathway, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-ascorbate from UDP-alpha-D-glucuronate, the pathway L-ascorbate biosynthesis via UDP-alpha-D-glucuronate pathway and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Ascorbate biosynthesis

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

UniPathwayiUPA00991; UER00939.

Names & Taxonomyi

Protein namesi
Recommended name:
L-gulonolactone oxidase (EC:1.1.3.8)
Short name:
LGO
Alternative name(s):
L-gulono-gamma-lactone oxidase
Short name:
GLO
Gene namesi
Name:GULO
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 8

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei253 – 27321HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 440439L-gulonolactone oxidasePRO_0000231673Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei54 – 541Pros-8alpha-FAD histidineBy similarity

Proteomic databases

PaxDbiQ3ZC33.
PRIDEiQ3ZC33.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000037993.

Structurei

3D structure databases

ProteinModelPortaliQ3ZC33.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini17 – 187171FAD-binding PCMH-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 FAD-binding PCMH-type domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4730. Eukaryota.
COG0277. LUCA.
GeneTreeiENSGT00510000049722.
HOGENOMiHOG000252847.
HOVERGENiHBG005834.
InParanoidiQ3ZC33.
KOiK00103.
OMAiCSETENV.
OrthoDBiEOG7N8ZV8.
TreeFamiTF328994.

Family and domain databases

Gene3Di3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
InterProiIPR007173. ALO.
IPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR010031. FAD_lactone_oxidase.
IPR006094. Oxid_FAD_bind_N.
IPR006093. Oxy_OxRdtase_FAD_BS.
IPR030654. Sugar_lactone_oxidase.
[Graphical view]
PfamiPF04030. ALO. 1 hit.
PF01565. FAD_binding_4. 1 hit.
[Graphical view]
PIRSFiPIRSF000136. LGO_GLO. 1 hit.
SUPFAMiSSF56176. SSF56176. 1 hit.
TIGRFAMsiTIGR01678. FAD_lactone_ox. 1 hit.
PROSITEiPS51387. FAD_PCMH. 1 hit.
PS00862. OX2_COVAL_FAD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3ZC33-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVHGYKGVKF QNWARTYGCC PEMYFQPTSV EEVREVLALA RQQNKRVKVV
60 70 80 90 100
GGGHSPSDIA CTDGFMIHMG KMNRVLKVDT EKKQVTVEAG ILLADLHPQL
110 120 130 140 150
DKHGLALSNL GAVSDVTAGG VIGSGTHNTG IKHGILATQV VALTLLTANG
160 170 180 190 200
TILECSESSN AEVFQAARVH LGCLGVILTV TLQCVPQFHL QETTFPSTLK
210 220 230 240 250
EVLDNLDSHL KKSEYFRFLW FPHSENVSVI YQDHTNKPPS SSANWFWDYA
260 270 280 290 300
IGFYLLEFLL WISTFLPGLV GWINRFFFWL LFNGKKENCN LSHKIFTYEC
310 320 330 340 350
RFKQHVQDWA IPREKTKEAL LELKAMLEAN PKVVAHYPVE VRFTRGDDIL
360 370 380 390 400
LSPCFQRDSC YMNIIMYRPY GKDVPRLDYW LAYETIMKKV GGRPHWAKAH
410 420 430 440
NCTRKDFEKM YPAFQRFCAI REKLDPTGMF LNAYLEKVFY
Length:440
Mass (Da):50,282
Last modified:January 23, 2007 - v3
Checksum:i8AAEE8413A80F048
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC102936 mRNA. Translation: AAI02937.1.
RefSeqiNP_001029215.1. NM_001034043.2.
UniGeneiBt.49608.

Genome annotation databases

EnsembliENSBTAT00000038177; ENSBTAP00000037993; ENSBTAG00000026748.
GeneIDi286812.
KEGGibta:286812.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC102936 mRNA. Translation: AAI02937.1.
RefSeqiNP_001029215.1. NM_001034043.2.
UniGeneiBt.49608.

3D structure databases

ProteinModelPortaliQ3ZC33.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000037993.

Proteomic databases

PaxDbiQ3ZC33.
PRIDEiQ3ZC33.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000038177; ENSBTAP00000037993; ENSBTAG00000026748.
GeneIDi286812.
KEGGibta:286812.

Organism-specific databases

CTDi268756.

Phylogenomic databases

eggNOGiKOG4730. Eukaryota.
COG0277. LUCA.
GeneTreeiENSGT00510000049722.
HOGENOMiHOG000252847.
HOVERGENiHBG005834.
InParanoidiQ3ZC33.
KOiK00103.
OMAiCSETENV.
OrthoDBiEOG7N8ZV8.
TreeFamiTF328994.

Enzyme and pathway databases

UniPathwayiUPA00991; UER00939.

Family and domain databases

Gene3Di3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
InterProiIPR007173. ALO.
IPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR010031. FAD_lactone_oxidase.
IPR006094. Oxid_FAD_bind_N.
IPR006093. Oxy_OxRdtase_FAD_BS.
IPR030654. Sugar_lactone_oxidase.
[Graphical view]
PfamiPF04030. ALO. 1 hit.
PF01565. FAD_binding_4. 1 hit.
[Graphical view]
PIRSFiPIRSF000136. LGO_GLO. 1 hit.
SUPFAMiSSF56176. SSF56176. 1 hit.
TIGRFAMsiTIGR01678. FAD_lactone_ox. 1 hit.
PROSITEiPS51387. FAD_PCMH. 1 hit.
PS00862. OX2_COVAL_FAD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Rumen reticulum.

Entry informationi

Entry nameiGGLO_BOVIN
AccessioniPrimary (citable) accession number: Q3ZC33
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 95 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.