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Protein

5-aminolevulinate synthase, erythroid-specific, mitochondrial

Gene

ALAS2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2.

Cofactori

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei163 – 1631SubstrateBy similarity
Binding sitei280 – 2801SubstrateBy similarity
Binding sitei299 – 2991SubstrateBy similarity
Binding sitei332 – 3321Pyridoxal phosphateBy similarity
Binding sitei360 – 3601Pyridoxal phosphateBy similarity
Binding sitei388 – 3881Pyridoxal phosphateBy similarity
Active sitei391 – 3911By similarity
Binding sitei420 – 4201Pyridoxal phosphateBy similarity
Binding sitei421 – 4211Pyridoxal phosphateBy similarity
Binding sitei508 – 5081SubstrateBy similarity

GO - Molecular functioni

  1. 5-aminolevulinate synthase activity Source: UniProtKB
  2. pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  1. cellular iron ion homeostasis Source: Ensembl
  2. erythrocyte differentiation Source: Ensembl
  3. hemoglobin biosynthetic process Source: Ensembl
  4. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
  5. response to hypoxia Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Heme biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

ReactomeiREACT_212036. Heme biosynthesis.
UniPathwayiUPA00251; UER00375.

Names & Taxonomyi

Protein namesi
Recommended name:
5-aminolevulinate synthase, erythroid-specific, mitochondrial (EC:2.3.1.37)
Short name:
ALAS-E
Alternative name(s):
5-aminolevulinic acid synthase 2
Delta-ALA synthase 2
Delta-aminolevulinate synthase 2
Gene namesi
Name:ALAS2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome X

Subcellular locationi

Mitochondrion matrix By similarity

GO - Cellular componenti

  1. mitochondrial inner membrane Source: UniProtKB
  2. mitochondrial matrix Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – ?4949MitochondrionSequence AnalysisAdd
BLAST
Chaini?50 – 5875385-aminolevulinate synthase, erythroid-specific, mitochondrialPRO_0000280705Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei391 – 3911N6-(pyridoxal phosphate)lysineCurated

Proteomic databases

PRIDEiQ3ZC31.

Interactioni

Subunit structurei

Homodimer. Interacts with SUCLA2 (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000017538.

Structurei

3D structure databases

ProteinModelPortaliQ3ZC31.
SMRiQ3ZC31. Positions 1-49, 145-540.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0156.
GeneTreeiENSGT00530000063111.
HOGENOMiHOG000221020.
HOVERGENiHBG005954.
InParanoidiQ3ZC31.
KOiK00643.
OMAiRAEKYIY.
OrthoDBiEOG7TQV0F.
TreeFamiTF300724.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR010961. 4pyrrol_synth_NH2levulA_synth.
IPR015118. 5aminolev_synth_preseq.
IPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
PF09029. Preseq_ALAS. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01821. 5aminolev_synth. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3ZC31-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTAAMLLQR CPVLIRSPTG LLGKMIKTHQ FLFGIGRCPI LATQGPSFSQ
60 70 80 90 100
IHLKATKAGG DSPSWAKSHC PFMLLELQDG KSKIVQKAAP EVQEDVKTFK
110 120 130 140 150
TDLPTSLAST SLKKTFSSPQ EPEKNSEKVT HLIQNNMAGD HVFGYDQFFR
160 170 180 190 200
DKIMEKKQDH TYRVFKTVNR WADAYPFAEH FFEASVASKD VSVWCSNDYL
210 220 230 240 250
GMSRHPRVLQ ATQEILQRHG AGAGGTRNIS GTSKFHVELE QELAELHQKD
260 270 280 290 300
SALLFSSCFV ANDSTLFTLA KILPGCEIYS DAGNHASMIQ GIRNSGAAKF
310 320 330 340 350
VFRHNDPDHL KKLLKKSNPE TPKIVAFETV HSMDGAICPL EELCDVAHQY
360 370 380 390 400
GALTFVDEVH AVGLYGSRGA GIGERDGIMH KIDIISGTLG KAFGCVGGYI
410 420 430 440 450
ASTRDLVDMV RSYAAGFIFT TSLPPMVLSG ALESVRLLKG EEGQALRRAH
460 470 480 490 500
QRNVKHMRQL LMDRGLPVIP CPSHIIPIRV GDAMLNTRIC DLLLSKYGIY
510 520 530 540 550
VQAINYPTVP RGEELLRLAP SPHHSPQMME DFVEKLLEAW TEVGLPLQDI
560 570 580
SIAACNFCRR PVHFELMSEW ERSYFGNMGP QYVTTYA
Length:587
Mass (Da):65,137
Last modified:September 27, 2005 - v1
Checksum:i8D0D94383137F5DC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC102938 mRNA. Translation: AAI02939.1.
RefSeqiNP_001030275.1. NM_001035103.2.
UniGeneiBt.49467.

Genome annotation databases

EnsembliENSBTAT00000017538; ENSBTAP00000017538; ENSBTAG00000013178.
GeneIDi511791.
KEGGibta:511791.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC102938 mRNA. Translation: AAI02939.1.
RefSeqiNP_001030275.1. NM_001035103.2.
UniGeneiBt.49467.

3D structure databases

ProteinModelPortaliQ3ZC31.
SMRiQ3ZC31. Positions 1-49, 145-540.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000017538.

Proteomic databases

PRIDEiQ3ZC31.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000017538; ENSBTAP00000017538; ENSBTAG00000013178.
GeneIDi511791.
KEGGibta:511791.

Organism-specific databases

CTDi212.

Phylogenomic databases

eggNOGiCOG0156.
GeneTreeiENSGT00530000063111.
HOGENOMiHOG000221020.
HOVERGENiHBG005954.
InParanoidiQ3ZC31.
KOiK00643.
OMAiRAEKYIY.
OrthoDBiEOG7TQV0F.
TreeFamiTF300724.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00375.
ReactomeiREACT_212036. Heme biosynthesis.

Miscellaneous databases

NextBioi20870099.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR010961. 4pyrrol_synth_NH2levulA_synth.
IPR015118. 5aminolev_synth_preseq.
IPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
PF09029. Preseq_ALAS. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01821. 5aminolev_synth. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Fetal liver.

Entry informationi

Entry nameiHEM0_BOVIN
AccessioniPrimary (citable) accession number: Q3ZC31
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: September 27, 2005
Last modified: January 7, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

There are two delta-ALA synthases in vertebrates: an erythroid- specific form and one (housekeeping) which is expressed in all tissues.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.