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Protein

Proliferating cell nuclear antigen

Gene

PCNA

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi61 – 80Sequence analysisAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair, DNA replication

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-BTA-110312. Translesion synthesis by REV1.
R-BTA-110314. Recognition of DNA damage by PCNA-containing replication complex.
R-BTA-110320. Translesion Synthesis by POLH.
R-BTA-113510. E2F mediated regulation of DNA replication.
R-BTA-1538133. G0 and Early G1.
R-BTA-174411. Polymerase switching on the C-strand of the telomere.
R-BTA-174414. Processive synthesis on the C-strand of the telomere.
R-BTA-174417. Telomere C-strand (Lagging Strand) Synthesis.
R-BTA-174437. Removal of the Flap Intermediate from the C-strand.
R-BTA-4615885. SUMOylation of DNA replication proteins.
R-BTA-5358565. Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha).
R-BTA-5651801. PCNA-Dependent Long Patch Base Excision Repair.
R-BTA-5655862. Translesion synthesis by POLK.
R-BTA-5656121. Translesion synthesis by POLI.
R-BTA-5656169. Termination of translesion DNA synthesis.
R-BTA-5685942. HDR through Homologous Recombination (HRR).
R-BTA-5696397. Gap-filling DNA repair synthesis and ligation in GG-NER.
R-BTA-5696400. Dual Incision in GG-NER.
R-BTA-6782135. Dual incision in TC-NER.
R-BTA-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-BTA-6804114. TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest.
R-BTA-69091. Polymerase switching.
R-BTA-69166. Removal of the Flap Intermediate.
R-BTA-69183. Processive synthesis on the lagging strand.
R-BTA-69205. G1/S-Specific Transcription.

Names & Taxonomyi

Protein namesi
Recommended name:
Proliferating cell nuclear antigen
Short name:
PCNA
Gene namesi
Name:PCNA
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 13

Subcellular locationi

  • Nucleus By similarity

  • Note: Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents. Colocalizes with CREBBP, EP300 and POLD1 to sites of DNA damage (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002849171 – 261Proliferating cell nuclear antigenAdd BLAST261

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei14N6-acetyllysineBy similarity1
Modified residuei77N6-acetyllysineBy similarity1
Modified residuei80N6-acetyllysineBy similarity1
Cross-linki164Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Cross-linki164Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Modified residuei211Phosphotyrosine; by EGFRBy similarity1
Modified residuei248N6-acetyllysineBy similarity1

Post-translational modificationi

Phosphorylated. Phosphorylation at Tyr-211 by EGFR stabilizes chromatin-associated PCNA (By similarity).By similarity
Acetylated by CREBBP and p300/EP300; preferentially acetylated by CREBBP on Lys-80, Lys-13 and Lys-14 and on Lys-77 by p300/EP300 upon loading on chromatin in response to UV irradiation. Lysine acetylation disrupts association with chromatin, hence promoting PCNA ubiquitination and proteasomal degradation in response to UV damage in a CREBBP- and EP300-dependent manner. Acetylation disrupts interaction with NUDT15 and promotes degradation (By similarity).By similarity
Ubiquitinated. Following DNA damage, can be either monoubiquitinated to stimulate direct bypass of DNA lesions by specialized DNA polymerases or polyubiquitinated to promote recombination-dependent DNA synthesis across DNA lesions by template switching mechanisms. Following induction of replication stress, monoubiquitinated by the UBE2B-RAD18 complex on Lys-164, leading to recruit translesion (TLS) polymerases, which are able to synthesize across DNA lesions in a potentially error-prone manner. An error-free pathway also exists and requires non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH. This error-free pathway, also known as template switching, employs recombination mechanisms to synthesize across the lesion, using as a template the undamaged, newly synthesized strand of the sister chromatid. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis. Sumoylated during S phase (By similarity).By similarity
Methylated on glutamate residues by ARMT1.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ3ZBW4.
PRIDEiQ3ZBW4.

Expressioni

Gene expression databases

BgeeiENSBTAG00000006065.

Interactioni

Subunit structurei

Homotrimer. Interacts with p300/EP300; the interaction occurs on chromatin in UV-irradiated damaged cells. Interacts with CREBBP (via transactivation domain and C-terminus); the interaction occurs on chromatin in UV-irradiated damaged cells. Directly interacts with POLD1, POLD3 and POLD4 subunits of the DNA polymerase delta complex, POLD3 being the major interacting partner; the interaction with POLD3 is inhibited by CDKN1A/p21(CIP1). Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with EXO1, POLH, POLK, DNMT1, ERCC5, FEN1, CDC6 and POLDIP2. Interacts with APEX2; this interaction is triggered by reactive oxygen species and increased by misincorporation of uracil in nuclear DNA. Forms a ternary complex with DNTTIP2 and core histone (By similarity). Interacts with KCTD10 and PPP1R15A (By similarity). Directly interacts with BAZ1B. Interacts with HLTF and SHPRH. Interacts with NUDT15; this interaction is disrupted in response to UV irradiation and acetylation. Interacts with CDKN1A/p21(CIP1) and CDT1; interacts via their PIP-box which also recruits the DCX(DTL) complex. The interaction with CDKN1A inhibits POLD3 binding. Interacts with DDX11. Interacts with EGFR; positively regulates PCNA. Interacts with PARPBP. Interacts (when ubiquitinated) with SPRTN; leading to enhance RAD18-mediated PCNA ubiquitination. Interacts (when polyubiquitinated) with ZRANB3. Interacts with SMARCAD1. Interacts with CDKN1C. Interacts with KIAA0101/PAF15 (via PIP-box). Interacts with RTEL1 (via PIP-box); the interaction is direct and essential for the suppression of telomere fragility. Interacts with FAM111A (via PIP-box); the interaction is direct and required for PCNA loading on chromatin binding. Interacts with LIG1. Interacts with SETMAR. Interacts with ANKRD17. Interacts with FBXO18/FBH1 (via PIP-box); the interaction recruits the DCX(DTL) complex and promotes ubiquitination and degradation of FBXO18/FBH1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi172122. 12 interactors.
STRINGi9913.ENSBTAP00000007967.

Structurei

3D structure databases

ProteinModelPortaliQ3ZBW4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PCNA family.Curated

Phylogenomic databases

eggNOGiKOG1636. Eukaryota.
COG0592. LUCA.
GeneTreeiENSGT00390000004965.
HOGENOMiHOG000211098.
HOVERGENiHBG000947.
InParanoidiQ3ZBW4.
KOiK04802.
OMAiSDGFDKY.
OrthoDBiEOG091G0GQ7.
TreeFamiTF313441.

Family and domain databases

HAMAPiMF_00317. DNApol_clamp_arch. 1 hit.
InterProiIPR000730. Pr_cel_nuc_antig.
IPR022649. Pr_cel_nuc_antig_C.
IPR022659. Pr_cel_nuc_antig_CS.
IPR022648. Pr_cel_nuc_antig_N.
[Graphical view]
PANTHERiPTHR11352. PTHR11352. 1 hit.
PfamiPF02747. PCNA_C. 1 hit.
PF00705. PCNA_N. 1 hit.
[Graphical view]
PRINTSiPR00339. PCNACYCLIN.
TIGRFAMsiTIGR00590. pcna. 1 hit.
PROSITEiPS01251. PCNA_1. 1 hit.
PS00293. PCNA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3ZBW4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFEARLVQGS ILKKVLEALK DLINEACWDI SSSGVNLQSM DSSHVSLVQL
60 70 80 90 100
TLRSEGFDTY RCDRNLAMGV NLTSMSKILK CAGNEDIITL RAEDNADTLA
110 120 130 140 150
LVFEAPNQEK VSDYEMKLMD LDVEQLGIPE QEYSCVVKMP SGEFARICRD
160 170 180 190 200
LSHIGDAVVI SCAKDGVKFS ASGELGNGNI KLSQTSNVDK EEEAVAIEMN
210 220 230 240 250
EPVQLTFALR YLNFFTKATP LSPTVTLSMS ADVPLVVEYK IADMGHLKYY
260
LAPKIEDEEG S
Length:261
Mass (Da):28,749
Last modified:September 27, 2005 - v1
Checksum:iD9D3E20EC7A8F9FD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC103068 mRNA. Translation: AAI03069.1.
RefSeqiNP_001029666.1. NM_001034494.1.
UniGeneiBt.52764.

Genome annotation databases

EnsembliENSBTAT00000007967; ENSBTAP00000007967; ENSBTAG00000006065.
GeneIDi515499.
KEGGibta:515499.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC103068 mRNA. Translation: AAI03069.1.
RefSeqiNP_001029666.1. NM_001034494.1.
UniGeneiBt.52764.

3D structure databases

ProteinModelPortaliQ3ZBW4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi172122. 12 interactors.
STRINGi9913.ENSBTAP00000007967.

Proteomic databases

PaxDbiQ3ZBW4.
PRIDEiQ3ZBW4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000007967; ENSBTAP00000007967; ENSBTAG00000006065.
GeneIDi515499.
KEGGibta:515499.

Organism-specific databases

CTDi5111.

Phylogenomic databases

eggNOGiKOG1636. Eukaryota.
COG0592. LUCA.
GeneTreeiENSGT00390000004965.
HOGENOMiHOG000211098.
HOVERGENiHBG000947.
InParanoidiQ3ZBW4.
KOiK04802.
OMAiSDGFDKY.
OrthoDBiEOG091G0GQ7.
TreeFamiTF313441.

Enzyme and pathway databases

ReactomeiR-BTA-110312. Translesion synthesis by REV1.
R-BTA-110314. Recognition of DNA damage by PCNA-containing replication complex.
R-BTA-110320. Translesion Synthesis by POLH.
R-BTA-113510. E2F mediated regulation of DNA replication.
R-BTA-1538133. G0 and Early G1.
R-BTA-174411. Polymerase switching on the C-strand of the telomere.
R-BTA-174414. Processive synthesis on the C-strand of the telomere.
R-BTA-174417. Telomere C-strand (Lagging Strand) Synthesis.
R-BTA-174437. Removal of the Flap Intermediate from the C-strand.
R-BTA-4615885. SUMOylation of DNA replication proteins.
R-BTA-5358565. Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha).
R-BTA-5651801. PCNA-Dependent Long Patch Base Excision Repair.
R-BTA-5655862. Translesion synthesis by POLK.
R-BTA-5656121. Translesion synthesis by POLI.
R-BTA-5656169. Termination of translesion DNA synthesis.
R-BTA-5685942. HDR through Homologous Recombination (HRR).
R-BTA-5696397. Gap-filling DNA repair synthesis and ligation in GG-NER.
R-BTA-5696400. Dual Incision in GG-NER.
R-BTA-6782135. Dual incision in TC-NER.
R-BTA-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-BTA-6804114. TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest.
R-BTA-69091. Polymerase switching.
R-BTA-69166. Removal of the Flap Intermediate.
R-BTA-69183. Processive synthesis on the lagging strand.
R-BTA-69205. G1/S-Specific Transcription.

Gene expression databases

BgeeiENSBTAG00000006065.

Family and domain databases

HAMAPiMF_00317. DNApol_clamp_arch. 1 hit.
InterProiIPR000730. Pr_cel_nuc_antig.
IPR022649. Pr_cel_nuc_antig_C.
IPR022659. Pr_cel_nuc_antig_CS.
IPR022648. Pr_cel_nuc_antig_N.
[Graphical view]
PANTHERiPTHR11352. PTHR11352. 1 hit.
PfamiPF02747. PCNA_C. 1 hit.
PF00705. PCNA_N. 1 hit.
[Graphical view]
PRINTSiPR00339. PCNACYCLIN.
TIGRFAMsiTIGR00590. pcna. 1 hit.
PROSITEiPS01251. PCNA_1. 1 hit.
PS00293. PCNA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPCNA_BOVIN
AccessioniPrimary (citable) accession number: Q3ZBW4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: September 27, 2005
Last modified: November 30, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.