ID   HAOX2_BOVIN             Reviewed;         353 AA.
AC   Q3ZBW2;
DT   01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   27-SEP-2005, sequence version 1.
DT   16-JUN-2009, entry version 36.
DE   RecName: Full=Hydroxyacid oxidase 2;
DE            Short=HAOX2;
DE            EC=1.1.3.15;
DE   AltName: Full=(S)-2-hydroxy-acid oxidase, peroxisomal;
GN   Name=HAO2;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia;
OC   Pecora; Bovidae; Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Fetal liver;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: (S)-2-hydroxy acid + O(2) = 2-oxo acid +
CC       H(2)O(2).
CC   -!- COFACTOR: FMN (By similarity).
CC   -!- SUBUNIT: Homotetramer or homooctamer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Peroxisome (By similarity).
CC   -!- SIMILARITY: Belongs to the FMN-dependent alpha-hydroxy acid
CC       dehydrogenase family.
CC   -!- SIMILARITY: Contains 1 FMN hydroxy acid dehydrogenase domain.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; BC103070; AAI03071.1; -; mRNA.
DR   IPI; IPI00709789; -.
DR   RefSeq; NP_001030243.1; -.
DR   UniGene; Bt.46051; -.
DR   SMR; Q3ZBW2; 2-350.
DR   Ensembl; ENSBTAG00000000123; Bos taurus.
DR   GeneID; 509481; -.
DR   KEGG; bta:509481; -.
DR   HOVERGEN; Q3ZBW2; -.
DR   OMA; Q3ZBW2; ITSTYAS.
DR   BRENDA; 1.1.3.15; 251.
DR   GO; GO:0005777; C:peroxisome; IEA:UniProtKB-SubCell.
DR   GO; GO:0003973; F:(S)-2-hydroxy-acid oxidase activity; IEA:EC.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0010181; F:FMN binding; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR012133; a-Hydoxy_acid_DH_FMN.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR000262; FMN-dep_DH.
DR   InterPro; IPR017934; FMN-dep_OHA_DH.
DR   InterPro; IPR008259; FMN_hydac_DH_AS.
DR   Gene3D; G3DSA:3.20.20.70; Aldolase_TIM; 1.
DR   Pfam; PF01070; FMN_dh; 1.
DR   PIRSF; PIRSF000138; Al-hdrx_acd_dh; 1.
DR   PROSITE; PS00557; FMN_HYDROXY_ACID_DH_1; 1.
DR   PROSITE; PS51349; FMN_HYDROXY_ACID_DH_2; 1.
PE   2: Evidence at transcript level;
KW   Flavoprotein; FMN; Oxidoreductase; Peroxisome.
FT   CHAIN         1    353       Hydroxyacid oxidase 2.
FT                                /FTId=PRO_0000285088.
FT   DOMAIN        1    353       FMN hydroxy acid dehydrogenase.
FT   NP_BIND     279    303       FMN (By similarity).
FT   MOTIF       351    353       Microbody targeting signal (Potential).
FT   ACT_SITE    248    248       Proton acceptor (By similarity).
FT   BINDING     106    106       FMN (By similarity).
FT   BINDING     128    128       FMN (By similarity).
FT   BINDING     130    130       Substrate (By similarity).
FT   BINDING     156    156       FMN (By similarity).
FT   BINDING     165    165       Substrate (By similarity).
FT   BINDING     224    224       FMN (By similarity).
FT   BINDING     251    251       Substrate (Potential).
SQ   SEQUENCE   353 AA;  39253 MW;  E20D09034252F692 CRC64;
     MPLVCLTDFR EHAREHLSKS TWDFIEGGAD DCCTRDENMA AFKKIRLRPR YLKDVSKVDM
     RTTIQGAEIS APICIAPTGF HRLAWPDGEM STARAAQAAS ICYITSTYAS CSLEDIVAAA
     PRGLRWFQLY VHPNRQINKQ MIQKVESLGF KALVITVDVP KVGNRRNDIT NQVDLMKKLL
     LKDLGSPEMG NVMPYFQMSP IDPSICWEDL SWFQSMTRLP IILKGILTKE DAELAVKHNV
     HGIIVSNHGG RQLDEVPASI DALTEVVAAV KGKVEVYLDG GIRTGNDVLK ALALGAKCVF
     VGRPILWGLA YKGEHGVKEV LDILKNEFHT SMTLTGCRSV AEINQDLIQF SRL
//