ID   SYTC_BOVIN              Reviewed;         723 AA.
AC   Q3ZBV8;
DT   11-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   27-SEP-2005, sequence version 1.
DT   16-JUN-2009, entry version 34.
DE   RecName: Full=Threonyl-tRNA synthetase, cytoplasmic;
DE            EC=6.1.1.3;
DE   AltName: Full=Threonine--tRNA ligase;
DE            Short=ThrRS;
GN   Name=TARS;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia;
OC   Pecora; Bovidae; Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Crossbred X Angus; TISSUE=Ileum;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: ATP + L-threonine + tRNA(Thr) = AMP +
CC       diphosphate + L-threonyl-tRNA(Thr).
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase
CC       family.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; BC103082; AAI03083.1; -; mRNA.
DR   IPI; IPI00711455; -.
DR   RefSeq; NP_001029542.1; -.
DR   UniGene; Bt.49332; -.
DR   PRIDE; Q3ZBV8; -.
DR   Ensembl; ENSBTAG00000014261; Bos taurus.
DR   GeneID; 510075; -.
DR   KEGG; bta:510075; -.
DR   HOVERGEN; Q3ZBV8; -.
DR   OMA; Q3ZBV8; KLWQTSG.
DR   BRENDA; 6.1.1.3; 251.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004829; F:threonine-tRNA ligase activity; IEA:EC.
DR   GO; GO:0006435; P:threonyl-tRNA aminoacylation; IEA:InterPro.
DR   InterPro; IPR002314; aa-tRNA-synt_IIb_cons-reg.
DR   InterPro; IPR006195; aa-tRNA-synth_II_cons-reg.
DR   InterPro; IPR004154; Anticodon_bd.
DR   InterPro; IPR012675; b-grasp_ferredoxin-like.
DR   InterPro; IPR004095; TGS.
DR   InterPro; IPR002320; Thr-tRNA-synth_IIa.
DR   InterPro; IPR018158; Thr-tRNA-synth_IIa_cons-reg.
DR   InterPro; IPR012947; tRNA_SAD.
DR   Gene3D; G3DSA:3.40.50.800; Anticodon_bd; 1.
DR   Gene3D; G3DSA:3.10.20.30; Ferredoxin_fold; 1.
DR   Pfam; PF03129; HGTP_anticodon; 1.
DR   Pfam; PF02824; TGS; 1.
DR   Pfam; PF00587; tRNA-synt_2b; 1.
DR   Pfam; PF07973; tRNA_SAD; 1.
DR   PRINTS; PR01047; TRNASYNTHTHR.
DR   TIGRFAMs; TIGR00418; thrS; 1.
DR   PROSITE; PS50862; AA_TRNA_LIGASE_II; 1.
PE   2: Evidence at transcript level;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Isopeptide bond;
KW   Ligase; Nucleotide-binding; Phosphoprotein; Protein biosynthesis;
KW   Ubl conjugation.
FT   CHAIN         1    723       Threonyl-tRNA synthetase, cytoplasmic.
FT                                /FTId=PRO_0000245024.
FT   MOD_RES     298    298       Phosphotyrosine (By similarity).
FT   CROSSLNK    222    222       Glycyl lysine isopeptide (Lys-Gly)
FT                                (interchain with G-Cter in ubiquitin) (By
FT                                similarity).
SQ   SEQUENCE   723 AA;  83492 MW;  58CC4E5B7BDC4C85 CRC64;
     MSEEQASSPS AKMGDEEKPV GAGEEKQKEG SKKKNKEGSG DGGRAELNPW PEYINTRLEM
     YNKLKAEHDS ILAEKAEKDS KPIKVTLPDG KQVDAESWKT TPYQIACGIS QGLADNTVIA
     KVNKAVWDLD RPLEEDCTLE LLKFEDEEAQ AVYWHSSAHI MGEAMERVYG GCLCYGPPIE
     NGFYYDMYLE EGGVSSNDFS SLETLCKKII KEKQAFERLE VKKETLLEMF KYNKFKCRIL
     NEKVNTPTTT VYRCGPLIDL CRGPHVRHTG KIKTLKIHKN SSTYWEGKSD METLQRIYGI
     SFPDPKMLKE WEKFQEEAKN RDHRKIGRDQ ELYFFHELSP GSCFFLPKGA YIYNTLIEFI
     RSEYRKRGFQ EVVTPNIYNS RLWVTSGHWE HYSENMFSFE VEKELFALKP MNCPGHCLMF
     DHRPRSWREL PLRVADFGVL HRNELSGALT GLTRVRRFQQ DDAHIFCAME QIEDEIKGCL
     DFLRTVYSIF GFSFKLNLST RPEKFLGDIE VWNQAEKQLE NSLNDFGEKW ELNPGDGAFY
     GPKIDIQIKD AIGRYHQCAT IQLDFQLPIR FNLTFVSHDG DDKKKPVIIH RAILGSVERM
     IAILTENYGG KWPFWLSPRQ VMVVPVGPTC DEYAQKVRQQ FHNAKFMVDI DLDPGCTLNK
     KIRNAQLAQY NFILVVGEKE KTSGTVNIRT RDNKVHGERT ISETIERLQQ LKHSRSKQAE
     EEF
//