Reviewed, UniProtKB/Swiss-Prot Q3ZBV8 (SYTC_BOVIN)

Names and origin

Protein names

Recommended name:
    Threonyl-tRNA synthetase, cytoplasmic
    EC=6.1.1.3
Alternative name(s):
    Threonine--tRNA ligase
      Short name=ThrRS

Gene names

Name:

TARS

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	723 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr).
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
PTM	Acetylation Isopeptide bond Phosphoprotein Ubl conjugation
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW threonine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 723	723	Threonyl-tRNA synthetase, cytoplasmic		PRO_0000245024
Amino acid modifications
	Modified residue	243	1	N6-acetyllysine By similarity
	Modified residue	298	1	Phosphotyrosine By similarity
	Cross-link	222		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3ZBV8-1 [UniParc]. Last modified September 27, 2005. Version 1. Checksum: 58CC4E5B7BDC4C85	FASTA	723	83,492
	10 20 30 40 50 60 MSEEQASSPS AKMGDEEKPV GAGEEKQKEG SKKKNKEGSG DGGRAELNPW PEYINTRLEM 70 80 90 100 110 120 YNKLKAEHDS ILAEKAEKDS KPIKVTLPDG KQVDAESWKT TPYQIACGIS QGLADNTVIA 130 140 150 160 170 180 KVNKAVWDLD RPLEEDCTLE LLKFEDEEAQ AVYWHSSAHI MGEAMERVYG GCLCYGPPIE 190 200 210 220 230 240 NGFYYDMYLE EGGVSSNDFS SLETLCKKII KEKQAFERLE VKKETLLEMF KYNKFKCRIL 250 260 270 280 290 300 NEKVNTPTTT VYRCGPLIDL CRGPHVRHTG KIKTLKIHKN SSTYWEGKSD METLQRIYGI 310 320 330 340 350 360 SFPDPKMLKE WEKFQEEAKN RDHRKIGRDQ ELYFFHELSP GSCFFLPKGA YIYNTLIEFI 370 380 390 400 410 420 RSEYRKRGFQ EVVTPNIYNS RLWVTSGHWE HYSENMFSFE VEKELFALKP MNCPGHCLMF 430 440 450 460 470 480 DHRPRSWREL PLRVADFGVL HRNELSGALT GLTRVRRFQQ DDAHIFCAME QIEDEIKGCL 490 500 510 520 530 540 DFLRTVYSIF GFSFKLNLST RPEKFLGDIE VWNQAEKQLE NSLNDFGEKW ELNPGDGAFY 550 560 570 580 590 600 GPKIDIQIKD AIGRYHQCAT IQLDFQLPIR FNLTFVSHDG DDKKKPVIIH RAILGSVERM 610 620 630 640 650 660 IAILTENYGG KWPFWLSPRQ VMVVPVGPTC DEYAQKVRQQ FHNAKFMVDI DLDPGCTLNK 670 680 690 700 710 720 KIRNAQLAQY NFILVVGEKE KTSGTVNIRT RDNKVHGERT ISETIERLQQ LKHSRSKQAE EEF

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Ileum.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC103082 mRNA. Translation: AAI03083.1.
IPI	IPI00711455.
RefSeq	NP_001029542.1.
UniGene	Bt.49332
3D structure databases
SMR	Q3ZBV8. Positions 81-153, 83-714, 321-721.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3ZBV8.
Proteomic databases
PRIDE	Q3ZBV8.
Genome annotation databases
Ensembl	ENSBTAT00000018947; ENSBTAP00000018947; ENSBTAG00000014261; Bos taurus. [Genome view]
GeneID	510075.
KEGG	bta:510075.
Organism-specific databases
CTD	510075.
Phylogenomic databases
eggNOG	maNOG11622.
HOVERGEN	Q3ZBV8.
InParanoid	Q3ZBV8.
OMA	HGARIYN.
OrthoDB	EOG92JR9S.
PhylomeDB	Q3ZBV8.
Enzyme and pathway databases
BRENDA	6.1.1.3. 251.
Family and domain databases
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II_cons-dom. IPR004154. Anticodon_bd. IPR012675. b-grasp_ferredoxin-like. IPR004095. TGS. IPR012676. TGS-like. IPR002320. Thr-tRNA-synth_IIa. IPR018158. Thr-tRNA-synth_IIa_cons-reg. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF02824. TGS. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00418. thrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYTC_BOVIN

Accession

Primary (citable) accession number: Q3ZBV8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2006
Last sequence update:	September 27, 2005
Last modified:	February 9, 2010
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)