ID   KCRS_BOVIN              Reviewed;         419 AA.
AC   Q3ZBP1;
DT   27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT   27-SEP-2005, sequence version 1.
DT   16-JUN-2009, entry version 36.
DE   RecName: Full=Creatine kinase, sarcomeric mitochondrial;
DE            EC=2.7.3.2;
DE   AltName: Full=S-MtCK;
DE   AltName: Full=Basic-type mitochondrial creatine kinase;
DE   AltName: Full=Mib-CK;
DE   Flags: Precursor;
GN   Name=CKMT2;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia;
OC   Pecora; Bovidae; Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Heart ventricle;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Reversibly catalyzes the transfer of phosphate between
CC       ATP and various phosphogens (e.g. creatine phosphate). Creatine
CC       kinase isoenzymes play a central role in energy transduction in
CC       tissues with large, fluctuating energy demands, such as skeletal
CC       muscle, heart, brain and spermatozoa (By similarity).
CC   -!- CATALYTIC ACTIVITY: ATP + creatine = ADP + phosphocreatine.
CC   -!- SUBUNIT: Exists as an octamer composed of four CKMT2 homodimers
CC       (By similarity).
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane; Peripheral
CC       membrane protein; Intermembrane side (By similarity).
CC   -!- MISCELLANEOUS: Mitochondrial creatine kinase binds cardiolipin.
CC   -!- SIMILARITY: Belongs to the ATP:guanido phosphotransferase family.
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DR   EMBL; BC103190; AAI03191.1; -; mRNA.
DR   IPI; IPI00695493; -.
DR   RefSeq; NP_001029828.1; -.
DR   UniGene; Bt.13980; -.
DR   SMR; Q3ZBP1; 47-413.
DR   Ensembl; ENSBTAG00000001003; Bos taurus.
DR   GeneID; 538944; -.
DR   KEGG; bta:538944; -.
DR   HOVERGEN; Q3ZBP1; -.
DR   BRENDA; 2.7.3.2; 251.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004111; F:creatine kinase activity; IEA:EC.
DR   InterPro; IPR000749; ATP-guanido_PTrfase.
DR   InterPro; IPR014746; Gln_synth/guanido_kin_cat.
DR   Gene3D; G3DSA:1.10.135.10; ATP-gua_Ptrans; 1.
DR   Gene3D; G3DSA:3.30.590.10; ATP-gua_Ptrans; 1.
DR   PANTHER; PTHR11547; ATP-gua_Ptrans; 1.
DR   Pfam; PF00217; ATP-gua_Ptrans; 1.
DR   Pfam; PF02807; ATP-gua_PtransN; 1.
DR   PROSITE; PS00112; GUANIDO_KINASE; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Kinase; Membrane; Mitochondrion;
KW   Mitochondrion inner membrane; Nucleotide-binding; Transferase;
KW   Transit peptide.
FT   TRANSIT       1     39       Mitochondrion (By similarity).
FT   CHAIN        40    419       Creatine kinase, sarcomeric
FT                                mitochondrial.
FT                                /FTId=PRO_0000244733.
FT   NP_BIND     162    166       ATP (By similarity).
FT   NP_BIND     354    359       ATP (By similarity).
FT   REGION       40     64       Cardiolipin-binding (By similarity).
FT   BINDING     225    225       ATP (By similarity).
FT   BINDING     270    270       ATP (By similarity).
FT   BINDING     326    326       ATP (By similarity).
FT   BINDING     369    369       ATP (By similarity).
SQ   SEQUENCE   419 AA;  47231 MW;  6C8D3F7622474D6F CRC64;
     MASTFSKLLT GRNASLLFAT LGTGALTTGY LLNKQNVCAA AREQHKLFPP SADYPDLRKH
     NNCMAECLTP AIYAKLRNKV TPSGYTLDQC IQTGVDNPGH PFIKTVGMVA GDEESYEVFA
     DLFDPVIKLR HNGYDPRVMK HPTDLDASKI TQGQFDERYV LSSRVRTGRS IRGLSLPPAC
     SRAELREVEN VAITALEGLK GDLAGRYYKL SEMTEQDQQR LIDDHFLFDK PVSPLLTCAG
     MARDWPDARG IWHNYDKTFL IWINEEDHTR VISMEKGGNM KRVFERFCRG LKEVERLIQE
     RGWEFMWNER LGYILTCPSN LGTGLRAGVH VRIPKLSKDP RFSKILENLR LQKRGTGGVD
     TAAVADVYDI SNIDRIGRSE VELVQIVIDG VNYLVDCEKK LERGQDIKVP PPLPQFGKK
//