Reviewed, UniProtKB/Swiss-Prot Q3ZBP1 (KCRS_BOVIN)

Names and origin

Protein names

Recommended name:
    Creatine kinase S-type, mitochondrial
    EC=2.7.3.2
Alternative name(s):
    Sarcomeric mitochondrial creatine kinase
      Short name=S-MtCK
    Basic-type mitochondrial creatine kinase
      Short name=Mib-CK

Gene names

Name:

CKMT2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa By similarity.
Catalytic activity	ATP + creatine = ADP + phosphocreatine.
Subunit structure	Exists as an octamer composed of four CKMT2 homodimers By similarity.
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side By similarity.
Miscellaneous	Mitochondrial creatine kinase binds cardiolipin.
Sequence similarities	Belongs to the ATP:guanido phosphotransferase family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW creatine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 39	39	Mitochondrion By similarity
	Chain	40 – 419	380	Creatine kinase S-type, mitochondrial		PRO_0000244733
Regions
	Nucleotide binding	162 – 166	5	ATP By similarity
	Nucleotide binding	354 – 359	6	ATP By similarity
	Region	40 – 64	25	Cardiolipin-binding By similarity
Sites
	Binding site	225	1	ATP By similarity
	Binding site	270	1	ATP By similarity
	Binding site	326	1	ATP By similarity
	Binding site	369	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3ZBP1-1 [UniParc]. Last modified September 27, 2005. Version 1. Checksum: 6C8D3F7622474D6F	FASTA	419	47,231
	10 20 30 40 50 60 MASTFSKLLT GRNASLLFAT LGTGALTTGY LLNKQNVCAA AREQHKLFPP SADYPDLRKH 70 80 90 100 110 120 NNCMAECLTP AIYAKLRNKV TPSGYTLDQC IQTGVDNPGH PFIKTVGMVA GDEESYEVFA 130 140 150 160 170 180 DLFDPVIKLR HNGYDPRVMK HPTDLDASKI TQGQFDERYV LSSRVRTGRS IRGLSLPPAC 190 200 210 220 230 240 SRAELREVEN VAITALEGLK GDLAGRYYKL SEMTEQDQQR LIDDHFLFDK PVSPLLTCAG 250 260 270 280 290 300 MARDWPDARG IWHNYDKTFL IWINEEDHTR VISMEKGGNM KRVFERFCRG LKEVERLIQE 310 320 330 340 350 360 RGWEFMWNER LGYILTCPSN LGTGLRAGVH VRIPKLSKDP RFSKILENLR LQKRGTGGVD 370 380 390 400 410 TAAVADVYDI SNIDRIGRSE VELVQIVIDG VNYLVDCEKK LERGQDIKVP PPLPQFGKK

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Heart ventricle.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC103190 mRNA. Translation: AAI03191.1.
IPI	IPI00695493.
RefSeq	NP_001029828.1.
UniGene	Bt.13980
3D structure databases
SMR	Q3ZBP1. Positions 47-413.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000001330; ENSBTAP00000001330; ENSBTAG00000001003; Bos taurus. [Genome view]
GeneID	538944.
KEGG	bta:538944.
Organism-specific databases
CTD	538944.
Phylogenomic databases
eggNOG	maNOG11792.
HOVERGEN	Q3ZBP1.
InParanoid	Q3ZBP1.
Enzyme and pathway databases
BRENDA	2.7.3.2. 251.
Family and domain databases
InterPro	IPR000749. ATP-guanido_PTrfase. IPR014746. Gln_synth/guanido_kin_cat_dom. [Graphical view]
Gene3D	G3DSA:1.10.135.10. ATP-gua_Ptrans. 1 hit. G3DSA:3.30.590.10. ATP-gua_Ptrans. 1 hit.
PANTHER	PTHR11547. ATP-gua_Ptrans. 1 hit.
Pfam	PF00217. ATP-gua_Ptrans. 1 hit. PF02807. ATP-gua_PtransN. 1 hit. [Graphical view]
PROSITE	PS00112. GUANIDO_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KCRS_BOVIN

Accession

Primary (citable) accession number: Q3ZBP1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	September 27, 2005
Last modified:	February 9, 2010
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)