Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase

Gene

ADI1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the formation of formate and 2-keto-4-methylthiobutyrate (KMTB) from 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene). Also down-regulates cell migration mediated by MMP14.UniRule annotation

Catalytic activityi

1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 = 4-(methylthio)-2-oxobutanoate + formate.UniRule annotation

Cofactori

Fe cationUniRule annotationNote: Binds 1 Fe cation per monomer. Can also use other divalent metal cations.UniRule annotation

Pathwayi: L-methionine biosynthesis via salvage pathway

This protein is involved in step 5 of the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Methylthioribose-1-phosphate isomerase (MRI1)
  2. Methylthioribulose-1-phosphate dehydratase (APIP)
  3. Enolase-phosphatase E1 (ENOPH1)
  4. Enolase-phosphatase E1 (ENOPH1)
  5. 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (ADI1)
  6. no protein annotated in this organism
This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate, the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi88 – 881Iron or nickelUniRule annotation
Metal bindingi90 – 901Iron or nickelUniRule annotation
Metal bindingi94 – 941Iron or nickelUniRule annotation
Metal bindingi133 – 1331Iron or nickelUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

ReactomeiR-BTA-1237112. Methionine salvage pathway.
UniPathwayiUPA00904; UER00878.

Names & Taxonomyi

Protein namesi
Recommended name:
1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenaseUniRule annotation (EC:1.13.11.54UniRule annotation)
Alternative name(s):
Acireductone dioxygenase (Fe(2+)-requiring)UniRule annotation
Short name:
ARDUniRule annotation
Short name:
Fe-ARDUniRule annotation
Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein 1UniRule annotation
Short name:
MTCBP-1UniRule annotation
Gene namesi
Name:ADI1UniRule annotation
Synonyms:MTCBP1UniRule annotation
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 8

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Nucleus UniRule annotation
  • Cell membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

  • Note: Localizes to the plasma membrane when complexed to MMP14.UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 1791791,2-dihydroxy-3-keto-5-methylthiopentene dioxygenasePRO_0000223187Add
BLAST

Proteomic databases

PaxDbiQ3ZBL1.
PRIDEiQ3ZBL1.

Interactioni

Subunit structurei

Monomer. Interacts with MMP14.UniRule annotation

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000007112.

Family & Domainsi

Sequence similaritiesi

Belongs to the acireductone dioxygenase (ARD) family.UniRule annotation

Phylogenomic databases

eggNOGiKOG2107. Eukaryota.
COG1791. LUCA.
GeneTreeiENSGT00390000008195.
HOGENOMiHOG000201071.
HOVERGENiHBG081992.
InParanoidiQ3ZBL1.
KOiK08967.
OMAiWIRIAVS.
OrthoDBiEOG73FQP0.
TreeFamiTF300231.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
HAMAPiMF_03154. Salvage_MtnD_euk.
InterProiIPR004313. ARD.
IPR027496. ARD_euk.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR23418. PTHR23418. 1 hit.
PfamiPF03079. ARD. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3ZBL1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVEAWYMDEA ADDPRLPHRA EPARPVGLEQ LRRLGVLYWK LDADKYENDP
60 70 80 90 100
ELEKIRKERN YSWVDIITIS KDKLPNYEEK IKMFFEEHLH LDEEIRYILD
110 120 130 140 150
GSGYFDVRDQ EDRWIRISME KGDMITLPAG IYHRFTLDEK NYVKAMRLFV
160 170
GDPVWTPYNR PSDHLEARTQ YLEFLAQSA
Length:179
Mass (Da):21,487
Last modified:December 14, 2011 - v2
Checksum:i5D9C500CC772CF60
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti7 – 71M → I in AAI03236 (Ref. 2) Curated
Sequence conflicti9 – 91E → D in AAI03236 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFC03041642 Genomic DNA. No translation available.
AAFC03041648 Genomic DNA. No translation available.
BC103235 mRNA. Translation: AAI03236.1.
RefSeqiNP_001030545.1. NM_001035468.2.
UniGeneiBt.49398.

Genome annotation databases

EnsembliENSBTAT00000007112; ENSBTAP00000007112; ENSBTAG00000005403.
GeneIDi615764.
KEGGibta:615764.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFC03041642 Genomic DNA. No translation available.
AAFC03041648 Genomic DNA. No translation available.
BC103235 mRNA. Translation: AAI03236.1.
RefSeqiNP_001030545.1. NM_001035468.2.
UniGeneiBt.49398.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000007112.

Proteomic databases

PaxDbiQ3ZBL1.
PRIDEiQ3ZBL1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000007112; ENSBTAP00000007112; ENSBTAG00000005403.
GeneIDi615764.
KEGGibta:615764.

Organism-specific databases

CTDi55256.

Phylogenomic databases

eggNOGiKOG2107. Eukaryota.
COG1791. LUCA.
GeneTreeiENSGT00390000008195.
HOGENOMiHOG000201071.
HOVERGENiHBG081992.
InParanoidiQ3ZBL1.
KOiK08967.
OMAiWIRIAVS.
OrthoDBiEOG73FQP0.
TreeFamiTF300231.

Enzyme and pathway databases

UniPathwayiUPA00904; UER00878.
ReactomeiR-BTA-1237112. Methionine salvage pathway.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
HAMAPiMF_03154. Salvage_MtnD_euk.
InterProiIPR004313. ARD.
IPR027496. ARD_euk.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR23418. PTHR23418. 1 hit.
PfamiPF03079. ARD. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of taurine cattle: a window to ruminant biology and evolution."
    The bovine genome sequencing and analysis consortium
    Science 324:522-528(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Hereford.
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Pancreas.

Entry informationi

Entry nameiMTND_BOVIN
AccessioniPrimary (citable) accession number: Q3ZBL1
Secondary accession number(s): F1N597
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: December 14, 2011
Last modified: July 6, 2016
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.