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Protein

1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase

Gene

ADI1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the formation of formate and 2-keto-4-methylthiobutyrate (KMTB) from 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene). Also down-regulates cell migration mediated by MMP14.UniRule annotation

Catalytic activityi

1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 = 4-(methylthio)-2-oxobutanoate + formate.UniRule annotation

Cofactori

Fe cationUniRule annotationNote: Binds 1 Fe cation per monomer. Can also use other divalent metal cations.UniRule annotation

Pathwayi: L-methionine biosynthesis via salvage pathway

This protein is involved in step 5 of the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Methylthioribose-1-phosphate isomerase (MRI1)
  2. Methylthioribulose-1-phosphate dehydratase (APIP)
  3. Enolase-phosphatase E1 (ENOPH1)
  4. Enolase-phosphatase E1 (ENOPH1)
  5. 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (ADI1)
  6. no protein annotated in this organism
This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate, the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi88Iron or nickelUniRule annotation1
Metal bindingi90Iron or nickelUniRule annotation1
Metal bindingi94Iron or nickelUniRule annotation1
Metal bindingi133Iron or nickelUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDioxygenase, Oxidoreductase
Biological processAmino-acid biosynthesis, Methionine biosynthesis
LigandIron, Metal-binding

Enzyme and pathway databases

ReactomeiR-BTA-1237112 Methionine salvage pathway
UniPathwayiUPA00904; UER00878

Names & Taxonomyi

Protein namesi
Recommended name:
1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenaseUniRule annotation (EC:1.13.11.54UniRule annotation)
Alternative name(s):
Acireductone dioxygenase (Fe(2+)-requiring)UniRule annotation
Short name:
ARDUniRule annotation
Short name:
Fe-ARDUniRule annotation
Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein 1UniRule annotation
Short name:
MTCBP-1UniRule annotation
Gene namesi
Name:ADI1UniRule annotation
Synonyms:MTCBP1UniRule annotation
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 8

Organism-specific databases

VGNCiVGNC:25675 ADI1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002231871 – 1791,2-dihydroxy-3-keto-5-methylthiopentene dioxygenaseAdd BLAST179

Proteomic databases

PaxDbiQ3ZBL1
PRIDEiQ3ZBL1

Expressioni

Gene expression databases

BgeeiENSBTAG00000005403

Interactioni

Subunit structurei

Monomer. Interacts with MMP14.UniRule annotation

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000007112

Family & Domainsi

Sequence similaritiesi

Belongs to the acireductone dioxygenase (ARD) family.UniRule annotation

Phylogenomic databases

eggNOGiKOG2107 Eukaryota
COG1791 LUCA
GeneTreeiENSGT00390000008195
HOGENOMiHOG000201071
HOVERGENiHBG081992
InParanoidiQ3ZBL1
KOiK08967
OMAiKERNYSW
OrthoDBiEOG091G0OM4
TreeFamiTF300231

Family and domain databases

Gene3Di2.60.120.10, 1 hit
HAMAPiMF_03154 Salvage_MtnD_euk, 1 hit
InterProiView protein in InterPro
IPR004313 ARD
IPR027496 ARD_euk
IPR014710 RmlC-like_jellyroll
IPR011051 RmlC_Cupin_sf
PANTHERiPTHR23418 PTHR23418, 1 hit
PfamiView protein in Pfam
PF03079 ARD, 1 hit
SUPFAMiSSF51182 SSF51182, 1 hit

Sequencei

Sequence statusi: Complete.

Q3ZBL1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVEAWYMDEA ADDPRLPHRA EPARPVGLEQ LRRLGVLYWK LDADKYENDP
60 70 80 90 100
ELEKIRKERN YSWVDIITIS KDKLPNYEEK IKMFFEEHLH LDEEIRYILD
110 120 130 140 150
GSGYFDVRDQ EDRWIRISME KGDMITLPAG IYHRFTLDEK NYVKAMRLFV
160 170
GDPVWTPYNR PSDHLEARTQ YLEFLAQSA
Length:179
Mass (Da):21,487
Last modified:December 14, 2011 - v2
Checksum:i5D9C500CC772CF60
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7M → I in AAI03236 (Ref. 2) Curated1
Sequence conflicti9E → D in AAI03236 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFC03041642 Genomic DNA No translation available.
AAFC03041648 Genomic DNA No translation available.
BC103235 mRNA Translation: AAI03236.1
RefSeqiNP_001030545.1, NM_001035468.2
UniGeneiBt.49398

Genome annotation databases

EnsembliENSBTAT00000007112; ENSBTAP00000007112; ENSBTAG00000005403
GeneIDi615764
KEGGibta:615764

Similar proteinsi

Entry informationi

Entry nameiMTND_BOVIN
AccessioniPrimary (citable) accession number: Q3ZBL1
Secondary accession number(s): F1N597
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: December 14, 2011
Last modified: March 28, 2018
This is version 95 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health