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Protein

DNA replication licensing factor MCM7

Gene

MCM7

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi381 – 3888ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Cell cycle, DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-BTA-176187. Activation of ATR in response to replication stress.
R-BTA-68867. Assembly of the pre-replicative complex.
R-BTA-68949. Orc1 removal from chromatin.
R-BTA-68962. Activation of the pre-replicative complex.
R-BTA-69052. Switching of origins to a post-replicative state.
R-BTA-69300. Removal of licensing factors from origins.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor MCM7 (EC:3.6.4.12)
Gene namesi
Name:MCM7
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 25

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 719718DNA replication licensing factor MCM7PRO_0000238630Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei121 – 1211PhosphoserineBy similarity
Modified residuei365 – 3651PhosphoserineBy similarity
Modified residuei500 – 5001PhosphoserineBy similarity

Post-translational modificationi

O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.By similarity

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ3ZBH9.
PRIDEiQ3ZBH9.

Interactioni

Subunit structurei

Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5. Interacts with the ATR-ATRIP complex and with RAD17. Interacts with TIPIN. Interacts with MCMBP (By similarity). Interacts with ANKRD17.By similarity

Protein-protein interaction databases

IntActiQ3ZBH9. 1 interaction.
STRINGi9913.ENSBTAP00000003728.

Structurei

3D structure databases

ProteinModelPortaliQ3ZBH9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini332 – 538207MCMAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni521 – 56444Interaction with RAD17By similarityAdd
BLAST
Regioni577 – 719143Interaction with ATRIPBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi513 – 5164Arginine finger

Sequence similaritiesi

Belongs to the MCM family.Curated
Contains 1 MCM domain.Curated

Phylogenomic databases

eggNOGiKOG0482. Eukaryota.
COG1241. LUCA.
GeneTreeiENSGT00670000098113.
HOGENOMiHOG000224125.
HOVERGENiHBG000741.
InParanoidiQ3ZBH9.
KOiK02210.
OMAiQTRACRF.
OrthoDBiEOG7N0C42.
TreeFamiTF300400.

Family and domain databases

Gene3Di2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR031327. MCM.
IPR008050. MCM7.
IPR018525. MCM_CS.
IPR001208. MCM_dom.
IPR027925. MCM_N.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11630:SF26. PTHR11630:SF26. 2 hits.
PfamiPF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
PR01663. MCMPROTEIN7.
SMARTiSM00382. AAA. 1 hit.
SM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 2 hits.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3ZBH9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALKDYVLEK DKVKKFLQEF YQDDESGKKQ FKYGNQLVQL AHREQVAMYV
60 70 80 90 100
DLDDIAEDDP ELVDSICENT KRYARLFADA VQELLPQYKE REVVNKDVLD
110 120 130 140 150
VYIEHRLMME QRSRDPGAAR SPQNQYPPEL MRRFELYFQG PSSNKPRVIR
160 170 180 190 200
EVRADSVGKL VTVRGIVTRV SEVKPRMVVA TYTCDQCGAE TYQPIQSPTF
210 220 230 240 250
MPLIMCPSQE CQTNRSGGRL YLQTRGSKFI KFQEMKMQEH SDQVPVGNIP
260 270 280 290 300
RSITVLVEGE NTRIAQPGDH VSVTGIFLPI LRTGFRQMVQ GLLSETYLEA
310 320 330 340 350
HRIVKMSKSE EDESGAGELT REELRQITEE DFYEKLAASI APEIYGHEDV
360 370 380 390 400
KKALLLLLVG GVDQSPRGMK IRGNINICLM GDPGVAKSQL LSYIDRLAPR
410 420 430 440 450
SQYTTGRGSS GVGLTAAVLR DSVSGELTLE GGALVLADQG VCCIDEFDKM
460 470 480 490 500
AEADRTAIHE VMEQQTISIA KAGILTTLNA RCSILAAANP AYGRYNPRRS
510 520 530 540 550
LEQNIQLPAA LLSRFDLLWL IQDRPDRDND LRLAQHITYV HQHSRQPPAQ
560 570 580 590 600
FEPLDMKLMR RYIAMCREKQ PAVPESLADY ITAAYVEMRR EAWASKDATY
610 620 630 640 650
TSARTLLAIL RLSTALARLR MVDTVEKEDV NEAIRLMEMS KDSLLGDKGQ
660 670 680 690 700
TARTQRPADV IFATVRELVS EGQSVRFSEA EQRCISRGFT PAQFQAALDE
710
YEELNVWQVN TARTRITFV
Length:719
Mass (Da):81,315
Last modified:September 27, 2005 - v1
Checksum:iB1EC2501A196B2AE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC103287 mRNA. Translation: AAI03288.1.
RefSeqiNP_001020516.2. NM_001025345.2.
UniGeneiBt.49518.

Genome annotation databases

EnsembliENSBTAT00000003728; ENSBTAP00000003728; ENSBTAG00000030965.
GeneIDi539924.
KEGGibta:539924.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC103287 mRNA. Translation: AAI03288.1.
RefSeqiNP_001020516.2. NM_001025345.2.
UniGeneiBt.49518.

3D structure databases

ProteinModelPortaliQ3ZBH9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ3ZBH9. 1 interaction.
STRINGi9913.ENSBTAP00000003728.

Proteomic databases

PaxDbiQ3ZBH9.
PRIDEiQ3ZBH9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000003728; ENSBTAP00000003728; ENSBTAG00000030965.
GeneIDi539924.
KEGGibta:539924.

Organism-specific databases

CTDi4176.

Phylogenomic databases

eggNOGiKOG0482. Eukaryota.
COG1241. LUCA.
GeneTreeiENSGT00670000098113.
HOGENOMiHOG000224125.
HOVERGENiHBG000741.
InParanoidiQ3ZBH9.
KOiK02210.
OMAiQTRACRF.
OrthoDBiEOG7N0C42.
TreeFamiTF300400.

Enzyme and pathway databases

ReactomeiR-BTA-176187. Activation of ATR in response to replication stress.
R-BTA-68867. Assembly of the pre-replicative complex.
R-BTA-68949. Orc1 removal from chromatin.
R-BTA-68962. Activation of the pre-replicative complex.
R-BTA-69052. Switching of origins to a post-replicative state.
R-BTA-69300. Removal of licensing factors from origins.

Family and domain databases

Gene3Di2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR031327. MCM.
IPR008050. MCM7.
IPR018525. MCM_CS.
IPR001208. MCM_dom.
IPR027925. MCM_N.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11630:SF26. PTHR11630:SF26. 2 hits.
PfamiPF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
PR01663. MCMPROTEIN7.
SMARTiSM00382. AAA. 1 hit.
SM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 2 hits.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Uterus.

Entry informationi

Entry nameiMCM7_BOVIN
AccessioniPrimary (citable) accession number: Q3ZBH9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: September 27, 2005
Last modified: June 8, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.