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Protein

DNA replication licensing factor MCM7

Gene

MCM7

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi381 – 388ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processCell cycle, DNA replication
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-BTA-176187 Activation of ATR in response to replication stress
R-BTA-68867 Assembly of the pre-replicative complex
R-BTA-68949 Orc1 removal from chromatin
R-BTA-68962 Activation of the pre-replicative complex
R-BTA-69052 Switching of origins to a post-replicative state

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor MCM7 (EC:3.6.4.12)
Gene namesi
Name:MCM7
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 25

Organism-specific databases

VGNCiVGNC:31313 MCM7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002386302 – 719DNA replication licensing factor MCM7Add BLAST718

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Cross-linki15Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki28Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei121PhosphoserineBy similarity1
Modified residuei314PhosphoserineBy similarity1
Modified residuei365PhosphoserineBy similarity1
Modified residuei500PhosphoserineBy similarity1
Modified residuei678PhosphoserineBy similarity1

Post-translational modificationi

O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.By similarity

Keywords - PTMi

Acetylation, Glycoprotein, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ3ZBH9
PeptideAtlasiQ3ZBH9
PRIDEiQ3ZBH9

Expressioni

Gene expression databases

BgeeiENSBTAG00000030965

Interactioni

Subunit structurei

Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5. Interacts with the ATR-ATRIP complex and with RAD17. Interacts with TIPIN. Interacts with MCMBP (By similarity). Interacts with ANKRD17. Component of the replisome complex composed of at least DONSON, MCM2, MCM7, PCNA and TICRR (By similarity).By similarity

Protein-protein interaction databases

IntActiQ3ZBH9, 1 interactor
STRINGi9913.ENSBTAP00000003728

Structurei

3D structure databases

ProteinModelPortaliQ3ZBH9
SMRiQ3ZBH9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini332 – 538MCMAdd BLAST207

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni521 – 564Interaction with RAD17By similarityAdd BLAST44
Regioni577 – 719Interaction with ATRIPBy similarityAdd BLAST143

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi513 – 516Arginine finger4

Sequence similaritiesi

Belongs to the MCM family.Curated

Phylogenomic databases

eggNOGiKOG0482 Eukaryota
COG1241 LUCA
GeneTreeiENSGT00900000141136
HOGENOMiHOG000224125
HOVERGENiHBG000741
InParanoidiQ3ZBH9
KOiK02210
OMAiVKIQEMA
OrthoDBiEOG091G02LB
TreeFamiTF300400

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR031327 MCM
IPR008050 MCM7
IPR018525 MCM_CS
IPR001208 MCM_dom
IPR027925 MCM_N
IPR033762 MCM_OB
IPR012340 NA-bd_OB-fold
IPR027417 P-loop_NTPase
PANTHERiPTHR11630 PTHR11630, 1 hit
PTHR11630:SF26 PTHR11630:SF26, 1 hit
PfamiView protein in Pfam
PF00493 MCM, 1 hit
PF14551 MCM_N, 1 hit
PF17207 MCM_OB, 1 hit
PRINTSiPR01657 MCMFAMILY
PR01663 MCMPROTEIN7
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SM00350 MCM, 1 hit
SUPFAMiSSF50249 SSF50249, 2 hits
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00847 MCM_1, 1 hit
PS50051 MCM_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3ZBH9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALKDYVLEK DKVKKFLQEF YQDDESGKKQ FKYGNQLVQL AHREQVAMYV
60 70 80 90 100
DLDDIAEDDP ELVDSICENT KRYARLFADA VQELLPQYKE REVVNKDVLD
110 120 130 140 150
VYIEHRLMME QRSRDPGAAR SPQNQYPPEL MRRFELYFQG PSSNKPRVIR
160 170 180 190 200
EVRADSVGKL VTVRGIVTRV SEVKPRMVVA TYTCDQCGAE TYQPIQSPTF
210 220 230 240 250
MPLIMCPSQE CQTNRSGGRL YLQTRGSKFI KFQEMKMQEH SDQVPVGNIP
260 270 280 290 300
RSITVLVEGE NTRIAQPGDH VSVTGIFLPI LRTGFRQMVQ GLLSETYLEA
310 320 330 340 350
HRIVKMSKSE EDESGAGELT REELRQITEE DFYEKLAASI APEIYGHEDV
360 370 380 390 400
KKALLLLLVG GVDQSPRGMK IRGNINICLM GDPGVAKSQL LSYIDRLAPR
410 420 430 440 450
SQYTTGRGSS GVGLTAAVLR DSVSGELTLE GGALVLADQG VCCIDEFDKM
460 470 480 490 500
AEADRTAIHE VMEQQTISIA KAGILTTLNA RCSILAAANP AYGRYNPRRS
510 520 530 540 550
LEQNIQLPAA LLSRFDLLWL IQDRPDRDND LRLAQHITYV HQHSRQPPAQ
560 570 580 590 600
FEPLDMKLMR RYIAMCREKQ PAVPESLADY ITAAYVEMRR EAWASKDATY
610 620 630 640 650
TSARTLLAIL RLSTALARLR MVDTVEKEDV NEAIRLMEMS KDSLLGDKGQ
660 670 680 690 700
TARTQRPADV IFATVRELVS EGQSVRFSEA EQRCISRGFT PAQFQAALDE
710
YEELNVWQVN TARTRITFV
Length:719
Mass (Da):81,315
Last modified:September 27, 2005 - v1
Checksum:iB1EC2501A196B2AE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC103287 mRNA Translation: AAI03288.1
RefSeqiNP_001020516.2, NM_001025345.2
UniGeneiBt.49518

Genome annotation databases

EnsembliENSBTAT00000003728; ENSBTAP00000003728; ENSBTAG00000030965
GeneIDi539924
KEGGibta:539924

Similar proteinsi

Entry informationi

Entry nameiMCM7_BOVIN
AccessioniPrimary (citable) accession number: Q3ZBH9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: September 27, 2005
Last modified: May 23, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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