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Protein

Proteasome subunit alpha type-7

Gene

PSMA7

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). Inhibits the transactivation function of HIF-1A under both normoxic and hypoxia-mimicking conditions. The interaction with EMAP2 increases the proteasome-mediated HIF-1A degradation under the hypoxic conditions. Plays a role in hepatitis C virus internal ribosome entry site-mediated translation. Mediates nuclear translocation of the androgen receptor (AR) and thereby enhances androgen-mediated transactivation. Promotes MAVS degradation and thereby negatively regulates MAVS-mediated innate immune response.By similarity

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Threonine protease

Enzyme and pathway databases

ReactomeiR-BTA-1169091. Activation of NF-kappaB in B cells.
R-BTA-1234176. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
R-BTA-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-BTA-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-BTA-174154. APC/C:Cdc20 mediated degradation of Securin.
R-BTA-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-BTA-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-BTA-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-BTA-195253. Degradation of beta-catenin by the destruction complex.
R-BTA-202424. Downstream TCR signaling.
R-BTA-2467813. Separation of Sister Chromatids.
R-BTA-2871837. FCERI mediated NF-kB activation.
R-BTA-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-BTA-350562. Regulation of ornithine decarboxylase (ODC).
R-BTA-382556. ABC-family proteins mediated transport.
R-BTA-446652. Interleukin-1 family signaling.
R-BTA-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-BTA-4608870. Asymmetric localization of PCP proteins.
R-BTA-4641257. Degradation of AXIN.
R-BTA-4641258. Degradation of DVL.
R-BTA-5358346. Hedgehog ligand biogenesis.
R-BTA-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-BTA-5607764. CLEC7A (Dectin-1) signaling.
R-BTA-5610780. Degradation of GLI1 by the proteasome.
R-BTA-5610785. GLI3 is processed to GLI3R by the proteasome.
R-BTA-5632684. Hedgehog 'on' state.
R-BTA-5658442. Regulation of RAS by GAPs.
R-BTA-5668541. TNFR2 non-canonical NF-kB pathway.
R-BTA-5676590. NIK-->noncanonical NF-kB signaling.
R-BTA-5687128. MAPK6/MAPK4 signaling.
R-BTA-5689603. UCH proteinases.
R-BTA-5689880. Ub-specific processing proteases.
R-BTA-68827. CDT1 association with the CDC6:ORC:origin complex.
R-BTA-68949. Orc1 removal from chromatin.
R-BTA-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-BTA-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-BTA-69481. G2/M Checkpoints.
R-BTA-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-BTA-8852276. The role of GTSE1 in G2/M progression after G2 checkpoint.
R-BTA-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-BTA-8939236. RUNX1 regulates transcription of genes involved in differentiation of HSCs.
R-BTA-8939902. Regulation of RUNX2 expression and activity.
R-BTA-8941858. Regulation of RUNX3 expression and activity.
R-BTA-8948751. Regulation of PTEN stability and activity.
R-BTA-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit alpha type-7 (EC:3.4.25.1By similarity)
Gene namesi
Name:PSMA7
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 13

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002740351 – 248Proteasome subunit alpha type-7Add BLAST248

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi130O-linked (GlcNAc) serineBy similarity1
Modified residuei153PhosphotyrosineBy similarity1
Modified residuei227N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ3ZBG0.
PeptideAtlasiQ3ZBG0.
PRIDEiQ3ZBG0.

Expressioni

Gene expression databases

BgeeiENSBTAG00000005127.

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is a barrel-shaped complex made of 28 subunits that are arranged in four stacked rings. The two outer rings are each formed by seven alpha subunits, and the two inner rings are formed by seven beta subunits. The proteolytic activity is exerted by three beta-subunits PSMB5, PSMB6 and PSMB7 (PubMed:12015144). PSMA7 interacts directly with the PSMG1-PSMG2 heterodimer which promotes 20S proteasome assembly (By similarity). Interacts with HIF1A (By similarity). Interacts with RAB7A (By similarity). Interacts with PRKN (By similarity). Interacts with ABL1 and ABL2 (By similarity). Interacts with EMAP2 (By similarity). Interacts with MAVS (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000006760.

Structurei

Secondary structure

1248
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi17 – 27Combined sources11
Beta strandi32 – 35Combined sources4
Beta strandi38 – 45Combined sources8
Beta strandi52 – 54Combined sources3
Helixi56 – 59Combined sources4
Beta strandi61 – 68Combined sources8
Beta strandi70 – 75Combined sources6
Helixi77 – 97Combined sources21
Beta strandi98 – 100Combined sources3
Helixi104 – 117Combined sources14
Beta strandi129 – 136Combined sources8
Beta strandi138 – 140Combined sources3
Beta strandi142 – 147Combined sources6
Beta strandi153 – 162Combined sources10
Helixi165 – 172Combined sources8
Turni173 – 175Combined sources3
Helixi184 – 196Combined sources13
Beta strandi208 – 215Combined sources8
Helixi222 – 228Combined sources7
Turni229 – 240Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IRUX-ray2.75D/R1-248[»]
ProteinModelPortaliQ3ZBG0.
SMRiQ3ZBG0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ3ZBG0.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1A family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0183. Eukaryota.
ENOG410XP21. LUCA.
GeneTreeiENSGT00550000074753.
HOGENOMiHOG000091085.
HOVERGENiHBG003005.
InParanoidiQ3ZBG0.
KOiK02731.
OMAiPYTQKPA.
OrthoDBiEOG091G0GX6.
TreeFamiTF106212.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiView protein in InterPro
IPR029055. Ntn_hydrolases_N.
IPR023332. Proteasome_alpha-type.
IPR035190. Proteasome_alpha7.
IPR000426. Proteasome_asu_N.
IPR001353. Proteasome_sua/b.
PANTHERiPTHR11599:SF40. PTHR11599:SF40. 1 hit.
PfamiView protein in Pfam
PF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
SMARTiView protein in SMART
SM00948. Proteasome_A_N. 1 hit.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiView protein in PROSITE
PS00388. PROTEASOME_ALPHA_1. 1 hit.
PS51475. PROTEASOME_ALPHA_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3ZBG0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYDRAITVF SPDGHLFQVE YAQEAVKKGS TAVGVRGKDI VVLGVEKKSV
60 70 80 90 100
AKLQDERTVR KICALDDNVC MAFAGLTADA RIVINRARVE CQSHRLTVED
110 120 130 140 150
PVTVEYITRY IASLKQRYTQ SNGRRPFGIS ALIVGFDFDG TPRLYQTDPS
160 170 180 190 200
GTYHAWKANA IGRGAKSVRE FLEKNYTDEA IETDDLTIKL VIKALLEVVQ
210 220 230 240
SGGKNIELAV MRRDQPLKIL NPEEIEKYVA EIEKEKEENE KKKQKKAS
Length:248
Mass (Da):27,869
Last modified:September 27, 2005 - v1
Checksum:iE38C9307D8E30D2C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC103328 mRNA. Translation: AAI03329.1.
RefSeqiNP_001029405.1. NM_001034233.2.
UniGeneiBt.49267.

Genome annotation databases

EnsembliENSBTAT00000006760; ENSBTAP00000006760; ENSBTAG00000005127.
GeneIDi505050.
KEGGibta:505050.

Similar proteinsi

Entry informationi

Entry nameiPSA7_BOVIN
AccessioniPrimary (citable) accession number: Q3ZBG0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: September 27, 2005
Last modified: November 22, 2017
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families