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Protein

Glucose-6-phosphate isomerase

Gene

GPI

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons (By similarity).By similarity

Catalytic activityi

D-glucose 6-phosphate = D-fructose 6-phosphate.

Pathwayi: glycolysis

This protein is involved in step 2 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (GPI)
  3. ATP-dependent 6-phosphofructokinase, liver type (PFKL), ATP-dependent 6-phosphofructokinase, muscle type (PFKM), ATP-dependent 6-phosphofructokinase (PFKP)
  4. Fructose-bisphosphate aldolase (ALDOB), Fructose-bisphosphate aldolase (ALDOC), Fructose-bisphosphate aldolase (ALDOA), Fructose-bisphosphate aldolase B (ALDOB)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei358Proton donorBy similarity1
Active sitei389By similarity1
Active sitei519By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCytokine, Isomerase
Biological processGluconeogenesis, Glycolysis

Enzyme and pathway databases

ReactomeiR-BTA-5628897 TP53 Regulates Metabolic Genes
R-BTA-6798695 Neutrophil degranulation
R-BTA-70171 Glycolysis
R-BTA-70263 Gluconeogenesis
SABIO-RKiQ3ZBD7
UniPathwayiUPA00109; UER00181

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate isomerase (EC:5.3.1.9)
Short name:
GPI
Alternative name(s):
Autocrine motility factor
Short name:
AMF
Neuroleukin
Short name:
NLK
Phosphoglucose isomerase
Short name:
PGI
Phosphohexose isomerase
Short name:
PHI
Gene namesi
Name:GPI
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 18

Organism-specific databases

VGNCiVGNC:29534 GPI

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002476412 – 557Glucose-6-phosphate isomeraseAdd BLAST556

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei12N6-acetyllysineBy similarity1
Modified residuei107PhosphoserineBy similarity1
Modified residuei142N6-acetyllysineBy similarity1
Modified residuei185Phosphoserine; by CK2By similarity1
Modified residuei250PhosphothreonineBy similarity1
Modified residuei455PhosphoserineBy similarity1

Post-translational modificationi

ISGylated.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ3ZBD7
PeptideAtlasiQ3ZBD7
PRIDEiQ3ZBD7

Expressioni

Gene expression databases

BgeeiENSBTAG00000006396

Interactioni

Subunit structurei

Homodimer in the catalytically active form, monomer in the secreted form.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000008386

Structurei

3D structure databases

SMRiQ3ZBD7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GPI family.Curated

Phylogenomic databases

eggNOGiKOG2446 Eukaryota
COG0166 LUCA
GeneTreeiENSGT00390000000707
HOGENOMiHOG000261370
HOVERGENiHBG002877
InParanoidiQ3ZBD7
KOiK01810
OMAiTNSQHAF
OrthoDBiEOG091G03ZI
TreeFamiTF300436

Family and domain databases

CDDicd05015 SIS_PGI_1, 1 hit
cd05016 SIS_PGI_2, 1 hit
Gene3Di1.10.1390.10, 1 hit
HAMAPiMF_00473 G6P_isomerase, 1 hit
InterProiView protein in InterPro
IPR001672 G6P_Isomerase
IPR023096 G6P_Isomerase_C
IPR018189 Phosphoglucose_isomerase_CS
IPR035476 SIS_PGI_1
IPR035482 SIS_PGI_2
PANTHERiPTHR11469 PTHR11469, 1 hit
PfamiView protein in Pfam
PF00342 PGI, 1 hit
PRINTSiPR00662 G6PISOMERASE
PROSITEiView protein in PROSITE
PS00765 P_GLUCOSE_ISOMERASE_1, 1 hit
PS00174 P_GLUCOSE_ISOMERASE_2, 1 hit
PS51463 P_GLUCOSE_ISOMERASE_3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3ZBD7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAALTQNPQF KKLKTWYEQH GSDLNLRRLF EGDRDRFNRF SLNLNTNHGH
60 70 80 90 100
ILVDYSKNLV TETVMQMLVD VAKSRGVEAA RERMFTGEKI NFTEDRAVLH
110 120 130 140 150
VALRNRSNAP ILVDGKDVMP EVNRVLEKMK SFCQRVRSGE WKGYSGKAIT
160 170 180 190 200
DVINIGIGGS DLGPLMVTEA LKPYSSEGPR VWFVSNIDGT HIAKTLATLN
210 220 230 240 250
PESSLFIIAS KTFTTQETIT NAETAKEWFL LSAKDPSAVA KHFVALSTNT
260 270 280 290 300
AKVKEFGIDP QNMFEFWDWV GGRYSLWSAI GLSIALHVGF DNFEQLLSGA
310 320 330 340 350
HWMDQHFRTT PLEKNAPVLL ALLGIWYINC FGCETHAMLP YDQYLHRFAA
360 370 380 390 400
YFQQGDMESN GKYITKSGTR VNYQTGPIVW GEPGTNGQHA FYQLIHQGTK
410 420 430 440 450
MIPCDFLIPV QSQHPIRNGL HHKILLANFL AQTEALMRGK STEEARKELQ
460 470 480 490 500
AAGRSPEDFE KLLPHKVFEG NRPTNSIVFT KLTPFILGAL IAMYEHKIFV
510 520 530 540 550
QGIIWDINSF DQWGVELGKQ LAKKIEPELD GSSPVTSHDS STNGLINFIK

QEREARS
Length:557
Mass (Da):62,855
Last modified:February 22, 2012 - v4
Checksum:iD932716D85FB2185
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti170A → S in AAI03417 (PubMed:19393038).Curated1
Sequence conflicti312L → WRRTP in AAB97860 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DAAA02046903 Genomic DNA No translation available.
BC103416 mRNA Translation: AAI03417.1
AF043228 mRNA Translation: AAB97860.1
RefSeqiNP_001035561.1, NM_001040471.1
UniGeneiBt.49587

Genome annotation databases

EnsembliENSBTAT00000008386; ENSBTAP00000008386; ENSBTAG00000006396
GeneIDi280808
KEGGibta:280808

Similar proteinsi

Entry informationi

Entry nameiG6PI_BOVIN
AccessioniPrimary (citable) accession number: Q3ZBD7
Secondary accession number(s): F1MD19, O46595
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: February 22, 2012
Last modified: April 25, 2018
This is version 93 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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