Reviewed, UniProtKB/Swiss-Prot Q3ZBD3 (PHS_BOVIN)

Names and origin

Protein names

Recommended name:
    Pterin-4-alpha-carbinolamine dehydratase
      Short name=PHS
    EC=4.2.1.96
Alternative name(s):
    4-alpha-hydroxy-tetrahydropterin dehydratase
    Phenylalanine hydroxylase-stimulating protein
    Pterin carbinolamine dehydratase
      Short name=PCD
    Dimerization cofactor of hepatocyte nuclear factor 1-alpha
      Short name=Dimerization cofactor of HNF1
      Short name=DCoH

Gene names

Name:

PCBD1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	104 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in tetrahydrobiopterin biosynthesis. Seems to both prevent the formation of 7-pterins and accelerate the formation of quinonoid-BH2 By similarity. Regulates the dimerization of homeodomain protein HNF-1-alpha and enhances its transcriptional activity By similarity.
Catalytic activity	(6R)-6-(L-erythro-1,2-dihydroxypropyl)-5,6,7,8-tetrahydro-4a-hydroxypterin = (6R)-6-(L-erythro-1,2-dihydroxypropyl)-7,8-dihydro-6H-pterin + H2O.
Subunit structure	Homotetramer or homodimer By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity. Note: Cytoplasmic and/or nuclear By similarity.
Sequence similarities	Belongs to the pterin-4-alpha-carbinolamine dehydratase family.

Ontologies

Keywords
Biological process	Tetrahydrobiopterin biosynthesis
Cellular component	Cytoplasm Nucleus
Molecular function	Lyase
PTM	Acetylation
Gene Ontology (GO)
Biological process	tetrahydrobiopterin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4-alpha-hydroxytetrahydrobiopterin dehydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 104	103	Pterin-4-alpha-carbinolamine dehydratase		PRO_0000315210
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3ZBD3-1 [UniParc]. Last modified January 15, 2008. Version 2. Checksum: 997DF8C2417FFD56	FASTA	104	11,986
	10 20 30 40 50 60 MAGKAHRLSA EERDQLLPNL RAVGWNELEG RDAIFKQFHF KDFNRAFGFM TRVALQAEKL 70 80 90 100 DHHPEWFNVY NKVHITLSTH ECAGLSERDV NLASFIEQVA VSMT

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Hypothalamus.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC103434 mRNA. Translation: AAI03435.1. Different initiation.
IPI	IPI00689238.
UniGene	Bt.48735
3D structure databases
SMR	Q3ZBD3. Positions 41-143.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000011866. Bos taurus. [Contig view]
Phylogenomic databases
HOVERGEN	Q3ZBD3.
Enzyme and pathway databases
BRENDA	4.2.1.96. 251.
Family and domain databases
InterPro	IPR001533. Trans/pterin_deHydtase. [Graphical view]
Gene3D	G3DSA:3.30.1360.20. Trans_pterinDh. 1 hit.
PANTHER	PTHR12599. Trans_pterinDh. 1 hit.
Pfam	PF01329. Pterin_4a. 1 hit. [Graphical view]
ProDom	PD007262. Trans_pterinDh. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

PHS_BOVIN

Accession

Primary (citable) accession number: Q3ZBD3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 15, 2008
Last modified:	June 16, 2009
This is version 31 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)