Reviewed, UniProtKB/Swiss-Prot Q3ZAF9 (KGUA_DEHE1)

Names and origin

Protein names

Recommended name:
    Guanylate kinase
    EC=2.7.4.8
Alternative name(s):
    GMP kinase

Gene names

Name:	gmk
Ordered Locus Names:	DET0035

Organism

Dehalococcoides ethenogenes (strain 195) [Complete proteome] [HAMAP]

Taxonomic identifier

243164 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Dehalococcoidetes › Dehalococcoides

Protein attributes

Sequence length	206 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Essential for recycling GMP and indirectly, cGMP By similarity. HAMAP MF_00328
Catalytic activity	ATP + GMP = ADP + GDP. HAMAP MF_00328
Subcellular location	Cytoplasm By similarity HAMAP MF_00328.
Sequence similarities	Belongs to the guanylate kinase family. Contains 1 guanylate kinase-like domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP guanylate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 206	206	Guanylate kinase HAMAP MF_00328		PRO_0000266314
Regions
	Domain	13 – 193	181	Guanylate kinase-like
	Nucleotide binding	20 – 27	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3ZAF9-1 [UniParc]. Last modified September 27, 2005. Version 1. Checksum: C574FCF176585596	FASTA	206	23,246
	10 20 30 40 50 60 MMTNWNFNKA NKPLLLVVSG PSGVGKDAVL ARMKERKLPL AYIVTTTTRT KREKETEGVD 70 80 90 100 110 120 YNFIRPAEFQ QLIGQNELLE WANVYGNFYG VPKAPIRQAL AHGFDVIVKV DVQGAASIKK 130 140 150 160 170 180 IVPNAVFIFL MPPDMDELTR RLEHRLTESP ESLKRRLATA PLEIEKLPDF DYVVVNPEGE 190 200 IDNAVSEIMS IISAEHCRIN PRSIEL

References

[1]

"Genome sequence of the PCE-dechlorinating bacterium Dehalococcoides ethenogenes." Seshadri R., Adrian L., Fouts D.E., Eisen J.A., Phillippy A.M., Methe B.A., Ward N.L., Nelson W.C., DeBoy R.T., Khouri H.M., Kolonay J.F., Dodson R.J., Daugherty S.C., Brinkac L.M., Sullivan S.A., Madupu R., Nelson K.E., Kang K.H. , Impraim M., Tran K., Robinson J.M., Forberger H.A., Fraser C.M., Zinder S.H., Heidelberg J.F. Science 307:105-108(2005) [PubMed: 15637277] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000027 Genomic DNA. Translation: AAW39120.1.
RefSeq	YP_180790.1.
3D structure databases
HSSP	HSSP built from PDB template 1ZNW based on UniProtKB P0A5I4.
SMR	Q3ZAF9. Positions 15-194.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3ZAF9.
Genome annotation databases
GeneID	3229077.
GenomeReviews	Gene locus DET0035 in contig CP000027_GR.
KEGG	det:DET0035.
NMPDR	fig|243164.3.peg.47.
TIGR	DET0035.
Phylogenomic databases
eggNOG	COG0194.
HOGENOM	HBG671982.
OMA	EWAEFAG.
Enzyme and pathway databases
BioCyc	DETH243164:DET_0035-MONOMER.
Family and domain databases
HAMAP	MF_00328. Guanylate_kinase. [Tree]
InterPro	IPR008144. Guanylate_kin. IPR008145. Guanylate_kin/L-typ_Ca_channel. IPR020590. Guanylate_kinase_CS. IPR017665. Guanylate_kinase_sub. [Graphical view]
Pfam	PF00625. Guanylate_kin. 1 hit. [Graphical view]
SMART	SM00072. GuKc. 1 hit. [Graphical view]
TIGRFAMs	TIGR03263. guanyl_kin. 1 hit.
PROSITE	PS00856. GUANYLATE_KINASE_1. 1 hit. PS50052. GUANYLATE_KINASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KGUA_DEHE1

Accession

Primary (citable) accession number: Q3ZAF9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	September 27, 2005
Last modified:	February 9, 2010
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)