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Protein

Ribosomal RNA small subunit methyltransferase A

Gene

rsmA

Organism
Dehalococcoides mccartyi (strain ATCC BAA-2266 / KCTC 15142 / 195) (Dehalococcoides ethenogenes (strain 195))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits.UniRule annotation

Catalytic activityi

4 S-adenosyl-L-methionine + adenine(1518)/adenine(1519) in 16S rRNA = 4 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(1518)/N(6)-dimethyladenine(1519) in 16S rRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei37 – 371S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei39 – 391S-adenosyl-L-methionine; via amide nitrogenUniRule annotation
Binding sitei64 – 641S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei85 – 851S-adenosyl-L-methionineUniRule annotation
Binding sitei110 – 1101S-adenosyl-L-methionineUniRule annotation
Binding sitei131 – 1311S-adenosyl-L-methionineUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciDETH243164:GJNF-404-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase AUniRule annotation (EC:2.1.1.182UniRule annotation)
Alternative name(s):
16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferaseUniRule annotation
16S rRNA dimethyladenosine transferaseUniRule annotation
16S rRNA dimethylaseUniRule annotation
S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferaseUniRule annotation
Gene namesi
Name:rsmAUniRule annotation
Synonyms:ksgAUniRule annotation
Ordered Locus Names:DET0404
OrganismiDehalococcoides mccartyi (strain ATCC BAA-2266 / KCTC 15142 / 195) (Dehalococcoides ethenogenes (strain 195))
Taxonomic identifieri243164 [NCBI]
Taxonomic lineageiBacteriaChloroflexiDehalococcoidiaDehalococcoidalesDehalococcoidaceaeDehalococcoides
Proteomesi
  • UP000008289 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 291291Ribosomal RNA small subunit methyltransferase APRO_0000257281Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243164.DET0404.

Structurei

3D structure databases

ProteinModelPortaliQ3Z9F0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D1X. Bacteria.
COG0030. LUCA.
HOGENOMiHOG000227962.
KOiK02528.
OMAiPHPAKDH.
OrthoDBiPOG091H02E3.

Family and domain databases

Gene3Di1.10.8.100. 1 hit.
3.40.50.150. 1 hit.
HAMAPiMF_00607. 16SrRNA_methyltr_A. 1 hit.
InterProiIPR001737. KsgA/Erm.
IPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11727. PTHR11727. 1 hit.
PfamiPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTiSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00755. ksgA. 1 hit.
PROSITEiPS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3Z9F0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKDAPLAAT GTPSLMAQAK EMMEGYTLRA RKGLGQHFLI SQGVLNKILL
60 70 80 90 100
AADLKPTDTV IEVGPGLGVL TEELIKRAGQ VIAVELDDKL VTALTEKFKA
110 120 130 140 150
YPNFRIIHSD ILKTSPAEIL GQNIPYKLVA NLPYYITSAV LRQFLEAEAK
160 170 180 190 200
PELMVVMVQK EVAKNMVAQT GDMGLLTLSV RFYGNPSLVS VVPGGAFYPP
210 220 230 240 250
PEVDSAIVKV AIPQAAIMQG VSEADFFKLA RAGFGTRRKT LLNALAQGLG
260 270 280 290
VSKQSVLALL NRAGIEPARR AETLSMEEWK MLCLVYTENP C
Length:291
Mass (Da):31,557
Last modified:September 27, 2005 - v1
Checksum:iA868A732A71A110B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000027 Genomic DNA. Translation: AAW40289.1.
RefSeqiWP_010936182.1. NC_002936.3.

Genome annotation databases

EnsemblBacteriaiAAW40289; AAW40289; DET0404.
GeneIDi3230253.
KEGGidet:DET0404.
PATRICi21607877. VBIDehEth89364_0383.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000027 Genomic DNA. Translation: AAW40289.1.
RefSeqiWP_010936182.1. NC_002936.3.

3D structure databases

ProteinModelPortaliQ3Z9F0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243164.DET0404.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAW40289; AAW40289; DET0404.
GeneIDi3230253.
KEGGidet:DET0404.
PATRICi21607877. VBIDehEth89364_0383.

Phylogenomic databases

eggNOGiENOG4105D1X. Bacteria.
COG0030. LUCA.
HOGENOMiHOG000227962.
KOiK02528.
OMAiPHPAKDH.
OrthoDBiPOG091H02E3.

Enzyme and pathway databases

BioCyciDETH243164:GJNF-404-MONOMER.

Family and domain databases

Gene3Di1.10.8.100. 1 hit.
3.40.50.150. 1 hit.
HAMAPiMF_00607. 16SrRNA_methyltr_A. 1 hit.
InterProiIPR001737. KsgA/Erm.
IPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11727. PTHR11727. 1 hit.
PfamiPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTiSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00755. ksgA. 1 hit.
PROSITEiPS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRSMA_DEHM1
AccessioniPrimary (citable) accession number: Q3Z9F0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: September 27, 2005
Last modified: September 7, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.