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Q3Z9C5 (SYV_DEHE1) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Valine--tRNA ligase
EC=6.1.1.9
Alternative name(s):
Valyl-tRNA synthetase
Short name=ValRS
Gene names
Name:valS
Ordered Locus Names:DET0430
OrganismDehalococcoides ethenogenes (strain 195) [Complete proteome] [HAMAP]
Taxonomic identifier243164 [NCBI]
Taxonomic lineageBacteria › Chloroflexi › Dehalococcoidetes › Dehalococcoides

Protein attributes

Sequence length880 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a "posttransfer" editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner By similarity. HAMAP MF_02004

Catalytic activity

ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val). HAMAP MF_02004

Subunit structure

Monomer By similarity. HAMAP MF_02004

Subcellular location

Cytoplasm By similarity HAMAP MF_02004.

Domain

ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site By similarity. HAMAP MF_02004

The C-terminal coiled-coil domain is crucial for aminoacylation activity By similarity. HAMAP MF_02004

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   DomainCoiled coil
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processvalyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

valine-tRNA ligase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 880880Valine--tRNA ligase HAMAP MF_02004
PRO_0000224471

Regions

Coiled coil815 – 85440 Potential
Motif51 – 6111"HIGH" region HAMAP MF_02004
Motif529 – 5335"KMSKS" region HAMAP MF_02004

Sites

Binding site5321ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q3Z9C5 [UniParc].

Last modified September 27, 2005. Version 1.
Checksum: 91B19D29BDDE3605

FASTA88099,965
        10         20         30         40         50         60 
MAQCSGLPEM AKAYEAAEVE KKWYQYWMEK GYFKPNPDSD KKPFVIIMPP PNVTGELHLG 

        70         80         90        100        110        120 
HALTATLEDI MIRWHRMLGE PALWLPGADH AGIAAQVVVE RMLAKQGKTR QELGRELFLE 

       130        140        150        160        170        180 
KMWEWVNPCR ERIRHQHMRL GASCDWDRET FTLDPGPVKA VREIFTNLYQ KGLIYRGERI 

       190        200        210        220        230        240 
INWCPRCATA VSDLEVDHKD LAGHIWHLRY PLEDGSGFVT VATTRPETML GDTAVAVHPD 

       250        260        270        280        290        300 
DARYTGMVGK NVLLPIMNRR IPVIADEAVD MAFGTGAVKV TPAHDPNDFE MGLRHSLPMI 

       310        320        330        340        350        360 
TIQNRDTTMN ENAGPCSGMT AKACREYVVS ELKSLGLLLK IEDYTHSVGH CQRCSAVIEP 

       370        380        390        400        410        420 
MVSKQWFVKM EPLAKPALEA VNSGRIQILP ERFTKVYQNW MENIRDWCIS RQLWWGHRIP 

       430        440        450        460        470        480 
VWYCPCGEMI VSKEDPTACP KCGSTKLEQD PDVLDTWFSS GLWPHSTLGW PDQTEDLKRF 

       490        500        510        520        530        540 
YPGSVLETAY DIIFFWVARM IVMGIEDMKE VPFRTVYLHG LIRDDKGEKM SKTKGNVIDP 

       550        560        570        580        590        600 
LKVIDQYGTD ALRFAVTFGT SPGNDSKLGQ TKLEAARNFV NKLWNASRFV IMNLGEEKEL 

       610        620        630        640        650        660 
LPEAGLPLED RWILSRMNRV TADVIRLMEE FQFGEAQRVL QDFVWGEFCD WYIELAKVRL 

       670        680        690        700        710        720 
RDEASVSPRP VLVKVLSTIL RLLHPYMPFI TEELWSYLRP YLPKSLGETD IIVAPFPQAD 

       730        740        750        760        770        780 
ETCFDEQAES IMGSLVEVVR SLRNLRAEHN VEISRYIQAN IYAGDMAEVL SNYLGAVETL 

       790        800        810        820        830        840 
SRSRPVNILP GHYSGASTAT EVVLVLNGIE VVVPMSTMVD LEAEAKRVEA EIAELETQIE 

       850        860        870        880 
RLSARLSDTQ FLAKAPQAVV DKERTKLEGY IEKVSRLKAV

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000027 Genomic DNA. Translation: AAW40282.1.
RefSeqYP_181174.1. NC_002936.3.

3D structure databases

ProteinModelPortalQ3Z9C5.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ3Z9C5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3230246.
GenomeReviewsGene locus DET0430 in contig CP000027_GR.
KEGGdet:DET0430.
NMPDRfig|243164.3.peg.566.
PATRIC21607929. VBIDehEth89364_0408.
TIGRDET0430.

Phylogenomic databases

eggNOGCOG0525.
HOGENOMHBG577712.
OMATDQWYVS.
PhylomeDBQ3Z9C5.
ProtClustDBPRK05729.

Enzyme and pathway databases

BioCycDETH243164:DET_0430-MONOMER.

Family and domain databases

HAMAPMF_02004. Val_tRNA_synth_type1.
[Tree]
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR014729. Rossmann-like_a/b/a_fold.
IPR010978. tRNA-bd_arm.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR011321. Val-tRNA_synth_Ia_C.
IPR019499. Val-tRNA_synth_Ia_tRNA-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR002303. Valyl-tRNA_synthetase.
[Graphical view]
Gene3DG3DSA:3.90.740.10. G3DSA:3.90.740.10. 1 hit.
G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
G3DSA:1.10.287.380. Val-tRNA_synth_Ia_C. 1 hit.
KOK01873.
PANTHERPTHR11946:SF5. tRNA-synt_val. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF10458. Val_tRNA-synt_C. 1 hit.
[Graphical view]
PRINTSPR00986. TRNASYNTHVAL.
SUPFAMSSF46589. tRNA_binding_arm. 1 hit.
SSF47323. tRNAsyn_1a_bind. 1 hit.
SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMsTIGR00422. ValS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYV_DEHE1
AccessionPrimary (citable) accession number: Q3Z9C5
Entry history
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: September 27, 2005
Last modified: January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

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