Q3Z9A4 (MDH_DEHE1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate dehydrogenase
EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	DET0451

Organism

Dehalococcoides ethenogenes (strain 195) [Complete proteome] [HAMAP]

Taxonomic identifier

243164 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Chloroflexi › Dehalococcoidetes › Dehalococcoidales › Dehalococcoidaceae › Dehalococcoides › Dehalococcoides mccartyi

Protein attributes

Sequence length	307 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_00487
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_00487
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular_function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 307	307	Malate dehydrogenase HAMAP-Rule MF_00487		PRO_0000241945
Regions
	Nucleotide binding	8 – 13	6	NAD By similarity
	Nucleotide binding	117 – 119	3	NAD By similarity
Sites
	Active site	174	1	Proton acceptor By similarity
	Binding site	32	1	NAD By similarity
	Binding site	81	1	Substrate By similarity
	Binding site	87	1	Substrate By similarity
	Binding site	94	1	NAD By similarity
	Binding site	119	1	Substrate By similarity
	Binding site	150	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3Z9A4 [UniParc]. Last modified September 27, 2005. Version 1. Checksum: 1E0E4DC4E660BCC7	FASTA	307	31,781
	10 20 30 40 50 60 MPKISVIGAG NVGATLAQRL IEKDFADVVM LDVVEGIPQG KALDISQSAN VLGFSHTITG 70 80 90 100 110 120 SNDYAETAGS EIVVITAGIA RKPGMTREEL LAINQKIMTD VVSNCLKYSP EATLVVVSNP 130 140 150 160 170 180 VDTMTYLAWK LSGLPRKRVV GLSGVLDGGR LATFVARELG VKPSAVTPCV MGEHGGSMVV 190 200 210 220 230 240 MPRFTLVSGK PLSELVSAEK ADELAKRAVN GGAEIVAFLK TGSAFYAPSA SIAAMAEAIF 250 260 270 280 290 300 TGSGKVMNCA AVLDGEYGLK NIVLGVPVKL GKGGLQEIIT LPLDGTENAR LLASAEVVKG QIAALSL

References

[1]

"Genome sequence of the PCE-dechlorinating bacterium Dehalococcoides ethenogenes." Seshadri R., Adrian L., Fouts D.E., Eisen J.A., Phillippy A.M., Methe B.A., Ward N.L., Nelson W.C., DeBoy R.T., Khouri H.M., Kolonay J.F., Dodson R.J., Daugherty S.C., Brinkac L.M., Sullivan S.A., Madupu R., Nelson K.E., Kang K.H. , Impraim M., Tran K., Robinson J.M., Forberger H.A., Fraser C.M., Zinder S.H., Heidelberg J.F. Science 307:105-108(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 195.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000027 Genomic DNA. Translation: AAW40235.1.
RefSeq	YP_181195.1. NC_002936.3.
3D structure databases
ProteinModelPortal	Q3Z9A4.
SMR	Q3Z9A4. Positions 3-305.
ModBase	Search...
Protein-protein interaction databases
STRING	243164.DET0451.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAW40235; AAW40235; DET0451.
GeneID	3230199.
KEGG	det:DET0451.
PATRIC	21607971. VBIDehEth89364_0429.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0039.
HOGENOM	HOG000213794.
KO	K00024.
OMA	NYKDIEG.
ProtClustDB	PRK06223.
Enzyme and pathway databases
BioCyc	DETH243164:GJNF-451-MONOMER.
Family and domain databases
Gene3D	3.40.50.720. 1 hit. 3.90.110.10. 1 hit.
HAMAP	MF_00487. Malate_dehydrog_3.
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR011275. Malate_DH_type3. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11540. PTHR11540. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01763. MalateDH_bact. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MDH_DEHE1

Accession

Primary (citable) accession number: Q3Z9A4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	September 27, 2005
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program