Reviewed, UniProtKB/Swiss-Prot Q3Z990 (AROE_DEHE1)

Names and origin

Protein names

Recommended name:
    Shikimate dehydrogenase
    EC=1.1.1.25

Gene names

Name:	aroE
Ordered Locus Names:	DET0465

Organism

Dehalococcoides ethenogenes (strain 195) [Complete proteome] [HAMAP]

Taxonomic identifier

243164 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Dehalococcoidetes › Dehalococcoides

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Shikimate + NADP+ = 3-dehydroshikimate + NADPH. HAMAP MF_00222
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and PEP: step 4/7. HAMAP MF_00222
Sequence similarities	Belongs to the shikimate dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro shikimate 5-dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 286	286	Shikimate dehydrogenase HAMAP MF_00222		PRO_1000058660
Regions
	Nucleotide binding	130 – 134	5	NADP By similarity
Sites
	Active site	70	1	Proton acceptor Potential

Sequences

	Sequence		Length	Mass (Da)
	Q3Z990-1 [UniParc]. Last modified September 27, 2005. Version 1. Checksum: 6ECE253437E7345F	FASTA	286	31,194
	10 20 30 40 50 60 MKTIPDALFG LIGFPVSHSV SPAMQNAAFK HCKLDYLYLT IAAKPEELQN VIASMGPLNI 70 80 90 100 110 120 RGLNVTIPHK IEVIKYIDSL DPAAEKIGAV NTIVNENGRL KGYNTDFGGF VRLLEHNRIA 130 140 150 160 170 180 PAKQRFTLLG AGGSAHAIAL AVCNLGGHLT VLARQEEKAK DLAGKMCLRL SGKTQGLELN 190 200 210 220 230 240 DTNLEEALAD TDVIVNCTPV GMGNLAGQSL VPPRLLRPDL TVIDAIYNPC KTRLLEDAEK 250 260 270 280 RGARIINGLE MLVWQGAMSF EIWTNQKAPF RLMMKEAELA LDENKK

References

[1]

"Genome sequence of the PCE-dechlorinating bacterium Dehalococcoides ethenogenes." Seshadri R., Adrian L., Fouts D.E., Eisen J.A., Phillippy A.M., Methe B.A., Ward N.L., Nelson W.C., DeBoy R.T., Khouri H.M., Kolonay J.F., Dodson R.J., Daugherty S.C., Brinkac L.M., Sullivan S.A., Madupu R., Nelson K.E., Kang K.H. , Impraim M., Tran K., Robinson J.M., Forberger H.A., Fraser C.M., Zinder S.H., Heidelberg J.F. Science 307:105-108(2005) [PubMed: 15637277] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000027 Genomic DNA. Translation: AAW40206.1.
RefSeq	YP_181209.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3230170.
GenomeReviews	Gene locus DET0465 in contig CP000027_GR.
KEGG	det:DET0465.
NMPDR	fig|243164.3.peg.727.
TIGR	DET0465.
Phylogenomic databases
HOGENOM	Q3Z990.
OMA	Q3Z990. ATAIQVQ.
Enzyme and pathway databases
BioCyc	DETH243164:DET_0465-MON.
Family and domain databases
HAMAP	MF_00222. [Tree]
InterPro	IPR016040. NAD(P)-bd_dom. IPR011342. Quinate/shikimate_5-DH. IPR013708. Shikimate_DH-bd_N. IPR006151. Shikm_DH/Glu-tRNA_Rdtase. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01488. Shikimate_DH. 1 hit. PF08501. Shikimate_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00507. aroE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AROE_DEHE1

Accession

Primary (citable) accession number: Q3Z990

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	September 27, 2005
Last modified:	June 16, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)