Reviewed, UniProtKB/Swiss-Prot Q3Z989 (AROD_DEHE1)

Names and origin

Protein names

Recommended name:
    3-dehydroquinate dehydratase
      Short name=3-dehydroquinase
    EC=4.2.1.10
Alternative name(s):
    Type I DHQase

Gene names

Name:	aroD
Ordered Locus Names:	DET0466

Organism

Dehalococcoides ethenogenes (strain 195) [Complete proteome] [HAMAP]

Taxonomic identifier

243164 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Dehalococcoidetes › Dehalococcoides

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	3-dehydroquinate = 3-dehydroshikimate + H2O. HAMAP MF_00214
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7. HAMAP MF_00214
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the type-I 3-dehydroquinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	3-dehydroquinate dehydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 222	222	3-dehydroquinate dehydratase HAMAP MF_00214		PRO_0000325522
Sites
	Active site	112	1	Proton acceptor By similarity
	Active site	139	1	Schiff-base intermediate with substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3Z989-1 [UniParc]. Last modified September 27, 2005. Version 1. Checksum: B174BD2BA2BC2266	FASTA	222	24,366
	10 20 30 40 50 60 MKNPPVCCVI TRLPEAESLK KSEGAAFYEL RLDLLGESWR EAAAMLDKPF MATCRRSAEG 70 80 90 100 110 120 GSFSGSEEER IGLLEKAAAA GAFMLDIEYS TPHLGEVLKR LRTQSKCLVS HHNFADTPSA 130 140 150 160 170 180 GDLKTLVKDM LNYPADIYKV ITTATSINDN IKLLNLIKEI PDKKIVAFAM GNLGILSRIL 190 200 210 220 CPLAGSPFTY ASLNDSNQSA SGQMTLAQMI EIYRSVNYEN HT

References

[1]

"Genome sequence of the PCE-dechlorinating bacterium Dehalococcoides ethenogenes." Seshadri R., Adrian L., Fouts D.E., Eisen J.A., Phillippy A.M., Methe B.A., Ward N.L., Nelson W.C., DeBoy R.T., Khouri H.M., Kolonay J.F., Dodson R.J., Daugherty S.C., Brinkac L.M., Sullivan S.A., Madupu R., Nelson K.E., Kang K.H. , Impraim M., Tran K., Robinson J.M., Forberger H.A., Fraser C.M., Zinder S.H., Heidelberg J.F. Science 307:105-108(2005) [PubMed: 15637277] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000027 Genomic DNA. Translation: AAW40205.1.
RefSeq	YP_181210.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3Z989.
Genome annotation databases
GeneID	3230169.
GenomeReviews	Gene locus DET0466 in contig CP000027_GR.
KEGG	det:DET0466.
NMPDR	fig|243164.3.peg.728.
TIGR	DET0466.
Phylogenomic databases
HOGENOM	Q3Z989.
OMA	LAPFTYA.
Enzyme and pathway databases
BioCyc	DETH243164:DET_0466-MON.
Family and domain databases
HAMAP	MF_00214. [Tree]
InterPro	IPR018508. 3-dehydroquinate_DH_AS. IPR013785. Aldolase_TIM. IPR001381. DHquinase_I. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01487. DHquinase_I. 1 hit. [Graphical view]
ProDom	PD005337. DHquinase_I. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01093. aroD. 1 hit.
PROSITE	PS01028. DEHYDROQUINASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROD_DEHE1

Accession

Primary (citable) accession number: Q3Z989

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	September 27, 2005
Last modified:	November 3, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)