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Q3Z8J1 (Q3Z8J1_DEHE1) Unreviewed, UniProtKB/TrEMBL

Last modified December 14, 2011. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518
Gene names
Name:topA EMBL AAW39972.1
Ordered Locus Names:DET0717
OrganismDehalococcoides ethenogenes (strain 195) [Complete proteome] [HAMAP]
Taxonomic identifier243164 [NCBI]
Taxonomic lineageBacteria › Chloroflexi › Dehalococcoidetes › Dehalococcoides

Protein attributes

Sequence length703 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733

Sequence similarities

Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206

Contains 1 Toprim domain. SAAS SAAS023406

Sequences

Sequence LengthMass (Da)Tools
Q3Z8J1 [UniParc].

Last modified September 27, 2005. Version 1.
Checksum: CA4BD5769D5D6A06

FASTA70379,712
        10         20         30         40         50         60 
MKEKLVIVES PAKARTISKM LGKDFNIMAT MGHIRDLPKS TLGVDVENSF TPKYVSLKAK 

        70         80         90        100        110        120 
AKVIKELKDA VKNSKAIYLA TDPDREGEAI AWHISEVTKA NLAKPKRVVF HEITKEAIDK 

       130        140        150        160        170        180 
AFKNTRELDM DLVNAQQARR VLDRLVGYKL SPLLWRKVQR GLSAGRVQSV SLKIIVDRER 

       190        200        210        220        230        240 
EIEKFVSAEY WNIEALLNKK VKSAAFKASL AGYVTKGKLE IHNESEAEQI KKELEVSDYQ 

       250        260        270        280        290        300 
VLKIKKKSNL RQSPAPFITS TLQQEAWRKL HFSAKQTMVI AQQLYEGLNI EGEGEVGLIT 

       310        320        330        340        350        360 
YMRTDSTNVA RTAVAETRDY ISEKYGPAYL PKQARVFSSK VKGAQEAHEA IRPTRIIRTP 

       370        380        390        400        410        420 
DLIKKSLSTE QFKLYQLIWQ RMMASQMSPA VFDNTTIDIE AKHQPSKTRY LLRTTSSVNT 

       430        440        450        460        470        480 
FPGFTIVYIE GKDEDEEAAQ SSLPPMEEGE ELKLKSLEAS QHFTQPPPRY TEATLIKALE 

       490        500        510        520        530        540 
QYGIGRPSTY APTISTIQER EYITKLKGSL KPSELGMLVN DLLVEYFPDI IGIDFTAAME 

       550        560        570        580        590        600 
TELDKIATEK LDWVPIIRRF YEPFIKEIEA ASEKIEKIKP PEEELDELCP ECNAKLVVKR 

       610        620        630        640        650        660 
GRFGKFIACS RYTKEEGGCR YTRSYQIKTG AKCPECNSDI VEKYSKKGKI FYGCAGYPNC 

       670        680        690        700 
KFVSFYKPLS KPCPECGALM IKSGKNWAKC TKCGKRKKLE DES

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000027 Genomic DNA. Translation: AAW39972.1.
RefSeqYP_181458.1. NC_002936.3.

3D structure databases

ProteinModelPortalQ3Z8J1.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ3Z8J1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3229934.
GenomeReviewsGene locus DET0717 in contig CP000027_GR.
KEGGdet:DET0717.
NMPDRfig|243164.3.peg.824.
PATRIC21608497. VBIDehEth89364_0691.
TIGRDET0717.

Phylogenomic databases

eggNOGCOG0550.
HOGENOMHBG512935.
OMAEEYWSID.
PhylomeDBQ3Z8J1.
ProtClustDBPRK05582.

Family and domain databases

InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR006171. Toprim_domain.
[Graphical view]
Gene3DG3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits.
G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KOK03168.
PANTHERPTHR11390. Topo_IA. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. Topo_IA_core. 1 hit.
TIGRFAMsTIGR01051. TopA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameQ3Z8J1_DEHE1
AccessionPrimary (citable) accession number: Q3Z8J1
Entry history
Integrated into UniProtKB/TrEMBL: September 27, 2005
Last sequence update: September 27, 2005
Last modified: December 14, 2011
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequences·References·Cross-refs·Entry info