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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Dehalococcoides mccartyi (strain ATCC BAA-2266 / KCTC 15142 / 195) (Dehalococcoides ethenogenes (strain 195))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei631ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciDMCC243164:G1G45-1003-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:DET1038
OrganismiDehalococcoides mccartyi (strain ATCC BAA-2266 / KCTC 15142 / 195) (Dehalococcoides ethenogenes (strain 195))
Taxonomic identifieri243164 [NCBI]
Taxonomic lineageiBacteriaChloroflexiDehalococcoidiaDehalococcoidalesDehalococcoidaceaeDehalococcoides
Proteomesi
  • UP000008289 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000985401 – 1014Isoleucine--tRNA ligaseAdd BLAST1014

Proteomic databases

PRIDEiQ3Z7P5

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi243164.DET1038

Structurei

3D structure databases

ProteinModelPortaliQ3Z7P5
SMRiQ3Z7P5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi48 – 58"HIGH" regionAdd BLAST11
Motifi628 – 632"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246403
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07961 Anticodon_Ia_Ile_ABEc, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02003 Ile_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR002300 aa-tRNA-synth_Ia
IPR033709 Anticodon_Ile_ABEc
IPR002301 Ile-tRNA-ligase
IPR023586 Ile-tRNA-ligase_type2
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit

Sequencei

Sequence statusi: Complete.

Q3Z7P5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKAVNPRQN FPQMEEDILK LWQDKGVFKK SIENRRDGKR FTLYEGPPTA
60 70 80 90 100
NGRPGIHHVL SRVFKDVIPR YKVMKGYYAP RIGGWDTHGL PVELEVEKEL
110 120 130 140 150
GFTSKNDIEK YGIAEFNARC RSSVFKYVSE WNKLTERIAY WVDLDNAYIT
160 170 180 190 200
MDNNYIESGW WALKQMWDKG LVYQGHRVTP HCPRCGTSLS SHEVAQGYKD
210 220 230 240 250
NTEDPSVFVK FEIAKESLAK AGLAKKWAYP ADKPLYLLAW TTTPWTLPAN
260 270 280 290 300
TALAVSAADQ YAILDMTDYY MILAKPRLSA LKLAENPVAG ECLGSDLSGL
310 320 330 340 350
FYKPLFDPRE FGIPVRNMQD NSETGVSEEL LYPVITTSYV SMDDGTGIVH
360 370 380 390 400
TAPAYGELDY ESGVKYGLKF VHHVDLQGRI TGSYPFAGKF VKEADKDISR
410 420 430 440 450
NLKERGLMFR NERMHHTYPF CWRCDSPLIY YAKQSWYIRT TAVRDELIKG
460 470 480 490 500
NQQINWYPEH IKDGRFGDWL ENNIDWAFSR ERYWGTPVPI WRCEKCGRTE
510 520 530 540 550
CVGGIDELKA KPNFKGMQEK LDIHRPYVDE WTYDCDKCGG NMKRVTEVMD
560 570 580 590 600
CWYDSGAMPV AQYHYPFEPE SRSIAKDGRF PADYICEAVD QTRGWFYSLH
610 620 630 640 650
AISTLIFNRP CYQNVICLGH ILDERGEKMS KSRNNVIQPA TVLDKYGADA
660 670 680 690 700
VRWYFYTAAP PGNARRFSEK LVGEVTRQFL LMLWNVYSFF VTYANIDSFT
710 720 730 740 750
PSEKYLEGEV PELDRWILSE LNQLVLDVDK GLDNYDPTQA GRRIEDFVGY
760 770 780 790 800
LSNWYVRRSR RRFWKSENDA DKLSAYQALY TCLVTLSRLL APFTPFVAEE
810 820 830 840 850
LYQNLVLSAD PSALESVHLT DFPVADTALI DEQLDNEIRL VMKVSSMGRS
860 870 880 890 900
ARSKAALKVR QPLAEVRVVL ASAGERTGLM RLAEQVLEEL NVKALAVEEP
910 920 930 940 950
GTVIPEKNYA ASTEGAYTVA VYTGLSPELL AEGTAREIVH RLQTMRKSAG
960 970 980 990 1000
FEIADYINTH YQADEYLDSV IRMHSEYIKK ETLSNQLIKG NAPEGAYAES
1010
LDIDGHSLSL WVAR
Length:1,014
Mass (Da):116,015
Last modified:September 27, 2005 - v1
Checksum:iDBA88E1091CC8AE2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000027 Genomic DNA Translation: AAW39707.1
RefSeqiWP_010936733.1, NC_002936.3

Genome annotation databases

EnsemblBacteriaiAAW39707; AAW39707; DET1038
GeneIDi3229669
KEGGidet:DET1038
PATRICifig|243164.10.peg.979

Similar proteinsi

Entry informationi

Entry nameiSYI_DEHM1
AccessioniPrimary (citable) accession number: Q3Z7P5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: September 27, 2005
Last modified: April 25, 2018
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health