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Q3Z7P5

- SYI_DEHM1

UniProt

Q3Z7P5 - SYI_DEHM1

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Dehalococcoides mccartyi (strain ATCC BAA-2266 / KCTC 15142 / 195) (Dehalococcoides ethenogenes (strain 195))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei631 – 6311ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciDETH243164:GJNF-1039-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:DET1038
OrganismiDehalococcoides mccartyi (strain ATCC BAA-2266 / KCTC 15142 / 195) (Dehalococcoides ethenogenes (strain 195))
Taxonomic identifieri243164 [NCBI]
Taxonomic lineageiBacteriaChloroflexiDehalococcoidiaDehalococcoidalesDehalococcoidaceaeDehalococcoides
ProteomesiUP000008289: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10141014Isoleucine--tRNA ligasePRO_0000098540Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi243164.DET1038.

Structurei

3D structure databases

ProteinModelPortaliQ3Z7P5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi48 – 5811"HIGH" regionAdd
BLAST
Motifi628 – 6325"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiRVEHMVE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3Z7P5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFKAVNPRQN FPQMEEDILK LWQDKGVFKK SIENRRDGKR FTLYEGPPTA
60 70 80 90 100
NGRPGIHHVL SRVFKDVIPR YKVMKGYYAP RIGGWDTHGL PVELEVEKEL
110 120 130 140 150
GFTSKNDIEK YGIAEFNARC RSSVFKYVSE WNKLTERIAY WVDLDNAYIT
160 170 180 190 200
MDNNYIESGW WALKQMWDKG LVYQGHRVTP HCPRCGTSLS SHEVAQGYKD
210 220 230 240 250
NTEDPSVFVK FEIAKESLAK AGLAKKWAYP ADKPLYLLAW TTTPWTLPAN
260 270 280 290 300
TALAVSAADQ YAILDMTDYY MILAKPRLSA LKLAENPVAG ECLGSDLSGL
310 320 330 340 350
FYKPLFDPRE FGIPVRNMQD NSETGVSEEL LYPVITTSYV SMDDGTGIVH
360 370 380 390 400
TAPAYGELDY ESGVKYGLKF VHHVDLQGRI TGSYPFAGKF VKEADKDISR
410 420 430 440 450
NLKERGLMFR NERMHHTYPF CWRCDSPLIY YAKQSWYIRT TAVRDELIKG
460 470 480 490 500
NQQINWYPEH IKDGRFGDWL ENNIDWAFSR ERYWGTPVPI WRCEKCGRTE
510 520 530 540 550
CVGGIDELKA KPNFKGMQEK LDIHRPYVDE WTYDCDKCGG NMKRVTEVMD
560 570 580 590 600
CWYDSGAMPV AQYHYPFEPE SRSIAKDGRF PADYICEAVD QTRGWFYSLH
610 620 630 640 650
AISTLIFNRP CYQNVICLGH ILDERGEKMS KSRNNVIQPA TVLDKYGADA
660 670 680 690 700
VRWYFYTAAP PGNARRFSEK LVGEVTRQFL LMLWNVYSFF VTYANIDSFT
710 720 730 740 750
PSEKYLEGEV PELDRWILSE LNQLVLDVDK GLDNYDPTQA GRRIEDFVGY
760 770 780 790 800
LSNWYVRRSR RRFWKSENDA DKLSAYQALY TCLVTLSRLL APFTPFVAEE
810 820 830 840 850
LYQNLVLSAD PSALESVHLT DFPVADTALI DEQLDNEIRL VMKVSSMGRS
860 870 880 890 900
ARSKAALKVR QPLAEVRVVL ASAGERTGLM RLAEQVLEEL NVKALAVEEP
910 920 930 940 950
GTVIPEKNYA ASTEGAYTVA VYTGLSPELL AEGTAREIVH RLQTMRKSAG
960 970 980 990 1000
FEIADYINTH YQADEYLDSV IRMHSEYIKK ETLSNQLIKG NAPEGAYAES
1010
LDIDGHSLSL WVAR
Length:1,014
Mass (Da):116,015
Last modified:September 27, 2005 - v1
Checksum:iDBA88E1091CC8AE2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000027 Genomic DNA. Translation: AAW39707.1.
RefSeqiWP_010936733.1. NC_002936.3.
YP_181754.1. NC_002936.3.

Genome annotation databases

EnsemblBacteriaiAAW39707; AAW39707; DET1038.
GeneIDi3229669.
KEGGidet:DET1038.
PATRICi21609123. VBIDehEth89364_0979.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000027 Genomic DNA. Translation: AAW39707.1 .
RefSeqi WP_010936733.1. NC_002936.3.
YP_181754.1. NC_002936.3.

3D structure databases

ProteinModelPortali Q3Z7P5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243164.DET1038.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAW39707 ; AAW39707 ; DET1038 .
GeneIDi 3229669.
KEGGi det:DET1038.
PATRICi 21609123. VBIDehEth89364_0979.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi RVEHMVE.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci DETH243164:GJNF-1039-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-2266 / KCTC 15142 / 195.

Entry informationi

Entry nameiSYI_DEHM1
AccessioniPrimary (citable) accession number: Q3Z7P5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: September 27, 2005
Last modified: October 29, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3