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Protein

Argininosuccinate synthase

Gene

argG

Organism
Dehalococcoides mccartyi (strain ATCC BAA-2266 / KCTC 15142 / 195) (Dehalococcoides ethenogenes (strain 195))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (argF)
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei86CitrullineUniRule annotation1
Binding sitei116ATP; via amide nitrogenUniRule annotation1
Binding sitei118AspartateUniRule annotation1
Binding sitei122AspartateUniRule annotation1
Binding sitei122CitrullineUniRule annotation1
Binding sitei123AspartateUniRule annotation1
Binding sitei126CitrullineUniRule annotation1
Binding sitei174CitrullineUniRule annotation1
Binding sitei183CitrullineUniRule annotation1
Binding sitei259CitrullineUniRule annotation1
Binding sitei271CitrullineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi8 – 16ATPUniRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processAmino-acid biosynthesis, Arginine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciDMCC243164:G1G45-1218-MONOMER
UniPathwayiUPA00068; UER00113

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:DET1260
OrganismiDehalococcoides mccartyi (strain ATCC BAA-2266 / KCTC 15142 / 195) (Dehalococcoides ethenogenes (strain 195))
Taxonomic identifieri243164 [NCBI]
Taxonomic lineageiBacteriaChloroflexiDehalococcoidiaDehalococcoidalesDehalococcoidaceaeDehalococcoides
Proteomesi
  • UP000008289 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002639181 – 406Argininosuccinate synthaseAdd BLAST406

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi243164.DET1260

Structurei

3D structure databases

ProteinModelPortaliQ3Z727
SMRiQ3Z727
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CDH Bacteria
COG0137 LUCA
HOGENOMiHOG000230093
KOiK01940
OMAiGVGRIDM
OrthoDBiPOG091H01U0

Family and domain databases

CDDicd01999 Argininosuccinate_Synthase, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.1260.10, 2 hits
HAMAPiMF_00005 Arg_succ_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001518 Arginosuc_synth
IPR018223 Arginosuc_synth_CS
IPR023434 Arginosuc_synth_type_1_subfam
IPR024074 AS_cat/multimer_dom_body
IPR014729 Rossmann-like_a/b/a_fold
PANTHERiPTHR11587 PTHR11587, 1 hit
PfamiView protein in Pfam
PF00764 Arginosuc_synth, 1 hit
TIGRFAMsiTIGR00032 argG, 1 hit
PROSITEiView protein in PROSITE
PS00564 ARGININOSUCCIN_SYN_1, 1 hit
PS00565 ARGININOSUCCIN_SYN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q3Z727-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEKVVLAYS GGLDTSAAVK WLQEKYGMDV IAVTIDVGNE KDFTLIKEKA
60 70 80 90 100
LKVGAKKAYV RDVRKEFAED YIWKAIKANS MYEGVYPLAT ALARPLIAKV
110 120 130 140 150
MVDIAMEEGA TAIAHGCTGK GNDQVRFDVG INTLAPHLKI IAPARQWGMT
160 170 180 190 200
REQTMEYAQK WGIPVPISVK NPFSIDENLW GRSIECGLLE DPWNEPIPEV
210 220 230 240 250
FAWTRPVEAT PDAPEYLEVE FEQGVPVAVN GEKLSPLALI QKVHDIAGLH
260 270 280 290 300
GVGRIDHVEN RLVGIKSREI YEAPAAVVLI AAHQALEAMT LSKSQLRFKQ
310 320 330 340 350
MVEATYSDII YNGLWFSALR QDLDAFIESS QRFVSGTVRL KLSKGSFRVV
360 370 380 390 400
GRKSPYSLYH KGMATYDKGD QFDPSSAVGF ITLWGLQAKL QAQLQPILEE

EKGNKS
Length:406
Mass (Da):45,044
Last modified:September 27, 2005 - v1
Checksum:i8FB43CE1B0AB9226
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000027 Genomic DNA Translation: AAW39470.1
RefSeqiWP_010936949.1, NC_002936.3

Genome annotation databases

EnsemblBacteriaiAAW39470; AAW39470; DET1260
GeneIDi3229430
KEGGidet:DET1260
PATRICifig|243164.10.peg.1189

Similar proteinsi

Entry informationi

Entry nameiASSY_DEHM1
AccessioniPrimary (citable) accession number: Q3Z727
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: September 27, 2005
Last modified: April 25, 2018
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health