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Protein

Glutamate 5-kinase

Gene

proB

Organism
Dehalococcoides mccartyi (strain ATCC BAA-2266 / KCTC 15142 / 195) (Dehalococcoides ethenogenes (strain 195))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (proB)
  2. Gamma-glutamyl phosphate reductase (proA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei12ATPUniRule annotation1
Binding sitei52SubstrateUniRule annotation1
Binding sitei139SubstrateUniRule annotation1
Binding sitei154Substrate; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi216 – 222ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processAmino-acid biosynthesis, Proline biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciDMCC243164:G1G45-1240-MONOMER
UniPathwayiUPA00098; UER00359

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:proBUniRule annotation
Ordered Locus Names:DET1282
OrganismiDehalococcoides mccartyi (strain ATCC BAA-2266 / KCTC 15142 / 195) (Dehalococcoides ethenogenes (strain 195))
Taxonomic identifieri243164 [NCBI]
Taxonomic lineageiBacteriaChloroflexiDehalococcoidiaDehalococcoidalesDehalococcoidaceaeDehalococcoides
Proteomesi
  • UP000008289 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002300431 – 373Glutamate 5-kinaseAdd BLAST373

Proteomic databases

PRIDEiQ3Z705

Interactioni

Protein-protein interaction databases

STRINGi243164.DET1282

Structurei

3D structure databases

ProteinModelPortaliQ3Z705
SMRiQ3Z705
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini281 – 359PUAUniRule annotationAdd BLAST79

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CGT Bacteria
COG0263 LUCA
HOGENOMiHOG000246369
KOiK00931
OMAiTHEIRFG
OrthoDBiPOG091H00OF

Family and domain databases

Gene3Di2.30.130.10, 1 hit
3.40.1160.10, 1 hit
HAMAPiMF_00456 ProB, 1 hit
InterProiView protein in InterPro
IPR036393 AceGlu_kinase-like_sf
IPR001048 Asp/Glu/Uridylate_kinase
IPR001057 Glu/AcGlu_kinase
IPR011529 Glu_5kinase
IPR005715 Glu_5kinase/COase_Synthase
IPR019797 Glutamate_5-kinase_CS
IPR002478 PUA
IPR015947 PUA-like_sf
IPR036974 PUA_sf
PfamiView protein in Pfam
PF00696 AA_kinase, 1 hit
PF01472 PUA, 1 hit
PIRSFiPIRSF000729 GK, 1 hit
PRINTSiPR00474 GLU5KINASE
SMARTiView protein in SMART
SM00359 PUA, 1 hit
SUPFAMiSSF53633 SSF53633, 1 hit
SSF88697 SSF88697, 1 hit
TIGRFAMsiTIGR01027 proB, 1 hit
PROSITEiView protein in PROSITE
PS00902 GLUTAMATE_5_KINASE, 1 hit
PS50890 PUA, 1 hit

Sequencei

Sequence statusi: Complete.

Q3Z705-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNGNCYKRIV IKLGTSLLTG GTGKLDHERM ADLCRQIADL TRLGTEVIIV
60 70 80 90 100
SSGAIAAGRA KMGLRHIPKD VPFKQVLAAI GQSQLMNYYD QLFSPHGLTV
110 120 130 140 150
AQGLLTKSDL SDRSGYLNAR NTLLALMELG VITIVNENDV VAVDEIQQAK
160 170 180 190 200
FGDNDNLSAM VANLIEADLL LILTNIRGLY TADPTLHPDA RLITEVKEIT
210 220 230 240 250
EELEKLAAGS SNKLGTGGMV TKLEAARLAT SSGVNVIIAD GHIPDIILKL
260 270 280 290 300
ASGETEGTRF MPSLHKPDSR QRWMMSGLCT RGNICIDDGA VKAIRENQKS
310 320 330 340 350
LLAAGVQQSE GKFGRGDIVK LSDSRGKRLG YGITNYSSDD VSKIKGLHTD
360 370
EINTVLAGNQ GPEIIHRNNL VVI
Length:373
Mass (Da):40,166
Last modified:September 27, 2005 - v1
Checksum:i4E494A9D82B01AF7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000027 Genomic DNA Translation: AAW39433.1
RefSeqiWP_010936971.1, NC_002936.3

Genome annotation databases

EnsemblBacteriaiAAW39433; AAW39433; DET1282
GeneIDi3229393
KEGGidet:DET1282
PATRICifig|243164.10.peg.1212

Similar proteinsi

Entry informationi

Entry nameiPROB_DEHM1
AccessioniPrimary (citable) accession number: Q3Z705
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: September 27, 2005
Last modified: April 25, 2018
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health